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Full-Text Articles in Life Sciences
Comparison Of Mesophyll Protoplast Isolation And Transformation Between Panicum Virgatum And Panicum Hallii, Andrea Grace Collins
Comparison Of Mesophyll Protoplast Isolation And Transformation Between Panicum Virgatum And Panicum Hallii, Andrea Grace Collins
Masters Theses
Protoplasts are appropriate targets for genome editing, DNA functional screens and transgenesis. This project focuses on the comparison of inexpensive mesophyll protoplast isolation via the use of food-grade enzymes and transformation between diploid Panicum hallii Vasey (PAH) and polyploid cellulosic feedstock Panicum virgatum L (switchgrass), a relative of PAH. PAH has great potential as a C4 model species for crop and bioenergy research. Here an inexpensive switchgrass and PAH mesophyll protoplast isolation and transformation system was developed; the first protoplast system for PAH. Using low-cost commercial food-grade enzymes, a cost reduction of ~1000-fold was achieved compared to traditional protoplast isolating …
Testing The Temporal Stability Of The Climate Response Of Tree Species At Norris Dam State Park, Tennessee, U.S.A., Allison Elizabeth Ingram
Testing The Temporal Stability Of The Climate Response Of Tree Species At Norris Dam State Park, Tennessee, U.S.A., Allison Elizabeth Ingram
Masters Theses
Temporal stability of the climate-tree growth relationship means that over time, tree species were responding to a specific climate variable and continue to respond to that variable into the present. The stability of this response is important to test prior to attempting to reconstruct past climate. In this study, I sampled oaks (white oak = Quercus alba L. and chestnut oak = Quercus montana Willd.) and pines (Virginia pine = Pinus virginiana Mill. and shortleaf pine = Pinus echinata Mill.) growing in Norris Dam State Park in eastern Tennessee and tested the temporal stability of these species and their potential …
Rooting Characteristics And Antioxidant Pigment Responses Of Three Hybrid Bermudagrass [Cynodon Dactylon (L.) Pers. × Cynodon Transvaalensis Burtt- Davy] Cultivars Exposed To Drought, Cory Alan Yurisic
Masters Theses
With recent changes in global climate, water use and its conservation have become important topics of discussion. Turfgrasses for lawns, parks, and other recreational areas are often perceived negatively due to the need for irrigation. The identification of turfgrasses possessing an improved ability to withstand water deficit is an important goal in science to curb irrigation requirements in the landscape. Newly released cultivars of hybrid bermudagrass (C. dactylon L. x. C. transvaalensis Burtt-Davy) have demonstrated improved tolerance to stresses such as cold, traffic, and water deficit; however, drought resistance mechanisms of these new cultivars have been largely uninvestigated. Drought …