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Plant Biology

Honors Theses

Western Michigan University

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Full-Text Articles in Life Sciences

Stability Of Eukaryotic Elongation Factor One Alpha Protein In The Cell, Jennifer Watry Apr 2007

Stability Of Eukaryotic Elongation Factor One Alpha Protein In The Cell, Jennifer Watry

Honors Theses

Eukaryotic Elongation Factor One Alpha (eEF1A) is an abundant protein found in every cell of every organism and is involved in functions concerning protein synthesis and degradation, the cytoskeleton, and signaling. How these functions are regulated is not yet understood for this protein. Regulation of the protein may be controlled by transcription of the genes encoding the protein or the stability and modification of the protein. The goal of this project was to determine the stability of eEf1A proteins in Arabidopsis thaliana. Protein stability was determined by treating tissues with cycloheximide, an inhibitor of protein synthesis. Gel electrophoresis and …


The Effects Of Rose Bengal Application On The Production Of Salicylic Acid In Nahg Tobacco Plants, Nicole Christine Ott Apr 1998

The Effects Of Rose Bengal Application On The Production Of Salicylic Acid In Nahg Tobacco Plants, Nicole Christine Ott

Honors Theses

Plants have the ability to develop resistance to a wide range of pathogens following exposure to a single bacterium, fungus, or virus. This resistance occurs in all tissues, not only those that were previously infected. This mechanisms is termed systemic acquired resistance (SAR). The phenolic compound salicylic acid (SA) has been linked to SAR in tobacco, arabidopsis, and cucumber plants. Oxidative stress also causes SA production, which leads to SAR. An engineered tobacco plant (nahG) that does not have the ability to accumulate SA has been used as a research tool in investigations concerning SA activity. Rose Bengal …