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In Vitro Propagation, Regeneration, Attempted Tetraploid Induction, And Agrobacterium-Mediated Transformation Of Euphorbia Pulchurrima ‘Winter Rose’™, Kimberly Ann Pickens
In Vitro Propagation, Regeneration, Attempted Tetraploid Induction, And Agrobacterium-Mediated Transformation Of Euphorbia Pulchurrima ‘Winter Rose’™, Kimberly Ann Pickens
Doctoral Dissertations
Poinsettia, Euphorbia pulchurrima, is the number one potted flowering plant in the United States. ‘Winter Rose’™ is a very popular cultivar with more than one million plants sold each year. To further improve this cultivar, particularly for larger flower heads and free branching, this research aimed at establishing some in vitro systems for application of biotechnology to poinsettia genetic improvement.
A protocol was established for in vitro axillary bud proliferation using greenhouse grown terminal buds. Buds were placed on Murashige-Skoog (MS) basal medium supplemented with benzlyaminopurine (BA). Explants produced the greatest number of axillary buds on medium containing between 2.2-8.8 …