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Full-Text Articles in Life Sciences
Microrna156: A Short Rna With A Major Role In Abiotic Stress Tolerance In Alfalfa, Biruk Ayenew Feyissa
Microrna156: A Short Rna With A Major Role In Abiotic Stress Tolerance In Alfalfa, Biruk Ayenew Feyissa
Electronic Thesis and Dissertation Repository
The highly conserved plant microRNA156, miR156, affects various aspects of plant development and stress response by silencing SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. Our understanding of the role of miR156 and its mode of action in alfalfa’s (Medicago sativa L.) response to drought and flooding is still elusive, and thus this study was aimed at filling this gap in knowledge. Physiological parameters, metabolite and transcriptional analyses showed an interplay between miR156/SPL13 and WD40-1/DFR to mitigate drought stress. Low to moderate levels of miR156 overexpression suppressed SPL13 and increased WD40-1 to fine-tune the DIHYDROFLAVONOL-4-REDUCTASE (DFR) level for …
Quantitative Proteome Analysis Of Alfalfa In Drought Stress Under The Influence Of Mir156, Alpa Puri
Quantitative Proteome Analysis Of Alfalfa In Drought Stress Under The Influence Of Mir156, Alpa Puri
Electronic Thesis and Dissertation Repository
Medicago sativa is one of the most produced perennial forage crops in Canada. Drought stress is a major form of abiotic stress affecting the productivity and annual yield of alfalfa. A small noncoding RNA, miR156 has a role in abiotic stress tolerance, while regulating downstream SPL genes. In this study, whole leaf proteome and amino acid concentrations of miR156OE (A8) genotype of alfalfa under drought stress were quantified. In results, 3,000 proteins groups were identified using MaxQuant when searched against Medicago truncatula protein sequence database. Gene ontology analysis showed enriched proteins were involved in biological and molecular processes such as …
Functional Analysis Of The Mir156 Regulatory Network In Arabidopsis Siliques, Zhishuo Wang
Functional Analysis Of The Mir156 Regulatory Network In Arabidopsis Siliques, Zhishuo Wang
Electronic Thesis and Dissertation Repository
Siliques are photosynthetically active seed capsules and their development is strongly influenced by embryo development. MicroRNA156 (miR156)-SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) network is involved in regulating plant growth and development, but the downstream genes of this network are still not fully elucidated. Here, I show that the miR156/SPL2 pathway controls the development of floral organs, regulates pollen production, and thus affects male fertility in Arabidopsis thaliana. I present evidence that SPL2 binds to the 5’UTR of the ASYMMETRIC LEAVES 2 (AS2) gene in vivo, indicating that AS2 acts downstream of SPL2. When compared to wild-type plants, AS2 loss-of-function mutants …