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Plant Biology

University of Wisconsin Milwaukee

Theses and Dissertations

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Full-Text Articles in Life Sciences

Descriptive Analyses Of Pollen Surface Morphologies In The Model Systems Brassica Rapa And Arabidopsis Thaliana And Three Arabidopsis Pollen Wall Mutants By Scanning Electron Microscopy, Andrew B. Kirkpatrick May 2015

Descriptive Analyses Of Pollen Surface Morphologies In The Model Systems Brassica Rapa And Arabidopsis Thaliana And Three Arabidopsis Pollen Wall Mutants By Scanning Electron Microscopy, Andrew B. Kirkpatrick

Theses and Dissertations

The mechanisms behind the construction of the pollen wall are equally elaborate and mysterious. Previous studies primarily used sectioned tissue to elucidate the events involved in proper pollen development. This study proposed and evaluated a protocol for exposing developing microspores to be examined by Scanning Electron Microscopy (SEM). Utilizing this protocol, comparative analyses of the superficial features present at the early, middle, and late tetrad as well as at released microspore stages of the model plants Brassica rapa and Arabidopsis thaliana were conducted. The utility of the technique was then evaluated through the examination of three Arabidopsis pollen wall mutants …


Arabidopsis Serk1 And 2 Regulate Anther Development As Co-Receptors Of Ems1, Yao Wang Dec 2013

Arabidopsis Serk1 And 2 Regulate Anther Development As Co-Receptors Of Ems1, Yao Wang

Theses and Dissertations

In flowering plants, male reproductive cell differentiation, one of the most critical events in the early stage of sexual reproduction, occurs during anther development. In the model plant species Arabidopsis, anther development in each lobe results in the differentiation of five highly organized cell layers with unique identities, including the central male reproductive cells and four somatic cell layers. These features make the Arabidopsis anther an ideal system in which to investigate the mechanisms of cell fate determination and differentiation. Previous studies have demonstrated that a leucine-rich repeat receptor-like kinase (LRR-RLK), EXCESS MICROSPOROCYTES1 (EMS1/EXS1), plays an important role during Arabidopsis …