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Developing Expressed Sequence Tag Libraries And The Discovery Of Simple Sequence Repeat Markers For Two Species Of Raspberry (Rubus L.), Jill M. Bushakra, Kim S. Lewers, Margaret E. Staton, Tetyana Zhebentyayeva, Christopher A. Saski
Developing Expressed Sequence Tag Libraries And The Discovery Of Simple Sequence Repeat Markers For Two Species Of Raspberry (Rubus L.), Jill M. Bushakra, Kim S. Lewers, Margaret E. Staton, Tetyana Zhebentyayeva, Christopher A. Saski
Entomology & Plant Pathology Publications and Other Works
Background
Due to a relatively high level of codominant inheritance and transferability within and among taxonomic groups, simple sequence repeat (SSR) markers are important elements in comparative mapping and delineation of genomic regions associated with traits of economic importance. Expressed sequence tags (ESTs) are a source of SSRs that can be used to develop markers to facilitate plant breeding and for more basic research across genera and higher plant orders.
Methods
Leaf and meristem tissue from ‘Heritage’ red raspberry (Rubus idaeus) and ‘Bristol’ black raspberry (R. occidentalis) were utilized for RNA extraction. After conversion to cDNA …