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Plant Biology

University of Nebraska - Lincoln

Transcriptome

2018

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

An Epigenetic Breeding System In Soybean For Increased Yield And Stability, Sunil K. Kenchanmane Raju, Mon-Ray Shao, Robersy Sanchez, Ying-Zhi Xu, Ajay Sandhu, George L. Graef, Sally A. Mackenzie Feb 2018

An Epigenetic Breeding System In Soybean For Increased Yield And Stability, Sunil K. Kenchanmane Raju, Mon-Ray Shao, Robersy Sanchez, Ying-Zhi Xu, Ajay Sandhu, George L. Graef, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Epigenetic variation has been associated with a wide range of adaptive phenotypes in plants, but there exist few direct means for exploiting this variation. RNAi suppression of the plant-specific gene, MutS HOMOLOG1 (MSH1), in multiple plant species produces a range of developmental changes accompanied by modulation of defence, phytohormone and abiotic stress response pathways along with methylome repatterning. This msh1-conditioned developmental reprogramming is retained independent of transgene segregation, giving rise to transgene-null ‘memory’ effects. An isogenic memory line crossed to wild type produces progeny families displaying increased variation in adaptive traits that respond to selection. This study …


Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault Jan 2018

Transcriptional Reprogramming Of Legume Genomes: Perspective And Challenges Associated With Single-Cell And Single Cell-Type Approaches During Nodule Development, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Transcriptomic approaches revealed thousands of genes differentially or specifically expressed during nodulation, a biological process resulting from the symbiosis between leguminous plant roots and rhizobia, atmospheric nitrogen-fixing symbiotic bacteria. Ultimately, nodulation will lead to the development of a new root organ, the nodule. Through functional genomic studies, plant transcriptomes have been used by scientists to reveal plant genes potentially controlling nodulation. However, it is important to acknowledge that the physiology, transcriptomic programs, and biochemical properties of the plant cells involved in nodulation are continuously regulated. They also differ between the different cell-types composing the nodules. To generate a more accurate …