Life Sciences Commons^™

Transcriptome Profiling Of Buffalograss Challenged With The Leaf Spot Pathogen Curvularia Inaequalis, Bimal S. Amaradasa, Keenan Amundsen

Department of Plant Pathology: Faculty Publications

Buffalograss (Bouteloua dactyloides) is a low maintenance U. S. native turfgrass species with exceptional drought, heat, and cold tolerance. Leaf spot caused by Curvularia inaequalis negatively impacts buffalograss visual quality. Two leaf spot susceptible and two resistant buffalograss lines were challenged with C. inaequalis. Samples were collected from treated and untreated leaves when susceptible lines showed symptoms. Transcriptome sequencing was done and differentially expressed genes were identified. Approximately 27 million raw sequencing reads were produced per sample. More than 86% of the sequencing reads mapped to an existing buffalograss reference transcriptome. De novo assembly of unmapped reads …

Transcriptome Profiling Of Kentucky Bluegrass (Poa Pratensis L.) Accessions In Response To Salt Stress, Shaun B. Bushman, Keenan L. Amundsen, Scott E. Warnke, Joseph G. Robins, Paul G. Johnson

Department of Agronomy and Horticulture: Faculty Publications

Background: Kentucky bluegrass (Poa pratensis L.) is a prominent turfgrass in the cool-season regions, but it is sensitive to salt stress. Previously, a relatively salt tolerant Kentucky bluegrass accession was identified that maintained green color under consistent salt applications. In this study, a transcriptome study between the tolerant (PI 372742) accession and a salt susceptible (PI 368233) accession was conducted, under control and salt treatments, and in shoot and root tissues.

Results: Sample replicates grouped tightly by tissue and treatment, and fewer differentially expressed transcripts were detected in the tolerant PI 372742 samples compared to the susceptible …