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Full-Text Articles in Life Sciences

Application Of Short Tandem Target Mimic (Sttm) Technique For Functional Analysis Of Micro-Rna396 In Transgenic Poplar Trees, Surattana Boonsai Jan 2019

Application Of Short Tandem Target Mimic (Sttm) Technique For Functional Analysis Of Micro-Rna396 In Transgenic Poplar Trees, Surattana Boonsai

Dissertations, Master's Theses and Master's Reports

Short Tandem Target Mimic (STTM) is a commonly technique used for functional studies of a number of genes in several plant model systems. However, very little is known about application of STTM technique in tree species. In this study, STTM was applied to knock down microRNA396 (miR396) in transgenic poplar trees for the first time. STTM396 expression resulted in dramatic decrease in miR396 expression levels leading to taller plants with larger leaves and larger leaf cell size. Additionally, an expression analysis of growth regulating factor genes (GRFs) that are members of miR396 target gene family showed up-regulation of GRF07 gene …


Ecophysiological Responses Of Tropical Woody Species To Ambient And Elevated Temperatures, Kelsey R. Carter Jan 2019

Ecophysiological Responses Of Tropical Woody Species To Ambient And Elevated Temperatures, Kelsey R. Carter

Dissertations, Master's Theses and Master's Reports

Tropical forests cycle one third of Earth’s carbon, yet we are still unsure how tropical vegetation will respond to climate warming. Tropical biomes experience a smaller temperature margin compared to other systems, possibly making them less capable of thermal adjustments. In addition, thermal responses of vegetation have been identified as one of the areas of greatest uncertainty for global carbon models. This dissertation works to quantify tropical forest photosynthetic responses to temperature as well as assessing physiological thermal acclimation of four tropical species. In Chapter, 2 we conducted a meta-analysis to investigate global tropical photosynthetic responses to temperature. We presented …


Biogeochemical Response To Vegetation And Hydrologic Change In An Alaskan Boreal Fen Ecosystem, Danielle L. Rupp Jan 2019

Biogeochemical Response To Vegetation And Hydrologic Change In An Alaskan Boreal Fen Ecosystem, Danielle L. Rupp

Dissertations, Master's Theses and Master's Reports

Boreal peatlands store approximately one third of the earth’s terrestrial carbon, locked away in currently waterlogged and frozen conditions. Peatlands of boreal and arctic ecosystems are affected increasingly by shifting hydrology caused by climate change. The consequences of these relatively rapid ecosystem changes on carbon cycling between the landscape and the atmosphere could provide an amplifying feedback to climate warming. Alternatively, the advancement of terrestrial vegetation into once waterlogged soils could uptake carbon as a sink. Previous work suggests that fens will become an increasingly dominant landscape feature in the boreal. However, studies investigating fens, their response to hydrologic and …