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Full-Text Articles in Life Sciences

The Identification Of Genetic Synteny Between Lolium Perenne Chromosome 7 And Rice Chromosome 6 Genomic Regions That Have Major Effects On Heading-Date, I. P. Armstead, L. B. Turner, L. Skøt, I. S. Donnison, M. O. Humphreys, I. P. King Apr 2023

The Identification Of Genetic Synteny Between Lolium Perenne Chromosome 7 And Rice Chromosome 6 Genomic Regions That Have Major Effects On Heading-Date, I. P. Armstead, L. B. Turner, L. Skøt, I. S. Donnison, M. O. Humphreys, I. P. King

IGC Proceedings (1997-2023)

Comparative genetic mapping between plant species has established that there has been a conservation of genomic organisation which reflects evolutionary relationships. The genetic mapping of L. perenne has identified such syntenic relationships with both the Triticeae and rice. The recent publication of the complete sequence of the rice genome has allowed these relationships to be analysed more closely and has raised the possibility of using the rice genome as a template for chromosome landing-based gene identification in related non-model species. The aim of the present work was to map particular markers and genes associated with heading-date in rice in L. …


An In Silico Dna Sequence Comparison Of The Perennial Ryegrass And Rice Genomes, Marty J. Faville Apr 2023

An In Silico Dna Sequence Comparison Of The Perennial Ryegrass And Rice Genomes, Marty J. Faville

IGC Proceedings (1997-2023)

Comparative mapping studies in the family Poaceae, which includes rice (a model species for this family) and perennial ryegrass (PRG) have indicated macro-colinearity of genes is generally conserved across different genomes. Genome mapping of simple sequence repeat markers derived from expressed sequence tags (EST-SSRs) for PRG (Faville et al., 2004) provides a vehicle for DNA sequence-based matching of mapped PRG genes to orthologous positions in the rice genome, which can be used to establish comparative relationships between these species’ genomes. We have initiated such an analysis using an EST-SSR-based PRG genome map. Our objective was to assess this in …


Decoding The Protein Interaction Network - An Approach Integrating Biology And Math, Ryan Huang, Lingkun Gu, Qingxi J. Shen Aug 2008

Decoding The Protein Interaction Network - An Approach Integrating Biology And Math, Ryan Huang, Lingkun Gu, Qingxi J. Shen

Undergraduate Research Opportunities Program (UROP)

The WRKY super family is known to play a major role during the plant stress response and development. My project focuses on the protein-protein interaction of an Oryzasativa (rice) transcription factor, OsWRKY71 which functions as the repressor of gibberellins signaling pathway. Previous literature revealed that OsWRKY71 can interact with itself or OsWRKY51 to form dimmers by using bimolecular fluorescence complementation (BiFC). To confirm this result, we use yeast two-hybrid system. As our data showed, OsWRKY71 seems to suppress the reporter gene expression of the conventional yeast two-hybrid system, so we use a modified yeast two-hybrid, Mating-based Split Ubiquitin System (MbSUS). …