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Full-Text Articles in Life Sciences

Rhythmic Growth And Vascular Development In Brachypodium Distachyon, Dominick A. Matos Jan 2012

Rhythmic Growth And Vascular Development In Brachypodium Distachyon, Dominick A. Matos

Masters Theses 1911 - February 2014

Plants reduce inorganic carbon to synthesize biomass that is comprised of mostly polysaccharides and lignin. Growth is intricately regulated by external cues such as light, temperature, and water availability and internal cues including those generated by the circadian clock. While many aspects of polymer biosynthesis are known, their regulation and distribution within the stem are poorly understood. Plant biomass is perhaps the most abundant organic substance on Earth and can be used as feedstock for energy production. Various grass species are under development as energy crops yet several of their attributes make them challenging research subjects. Brachypodium distachyon has emerged …


Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells, Michael E. Urbanowski Jan 2012

Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells, Michael E. Urbanowski

Masters Theses 1911 - February 2014

The regulation of pH is a critical homeostatic function of plant cells. In addition to acting as the primary cationic species responsible for energizing the plasma membrane, protons likely act as an important regulator and messenger. Despite this importance, few studies have thoroughly described cytosolic pH patterns as the plant cell progresses through the cell cycle. To investigate pH in plant cells, I chose Nicotiana tabacum (tobacco) Bright Yellow-2 (BY-2) cells as a model system. My research has two aims. First, I will measure and report the interphase cytosolic pH of BY-2 cells. Next, I will assay the cytosolic pH …