Life Sciences Commons^™

Role Of Eps15 Homology Domain-Containing Protein 4 (Ehd4) In The Kidney, Shamma Rahman

Theses & Dissertations

In the kidney, endocytic recycling regulates the abundance of channels and transporters in the membrane of the tubular epithelium, and thereby controls the kidney’s ability to regulate water homeostasis. In recent years, a family of proteins called Eps15 homology domain-containing (EHD) proteins has emerged as important regulators of the endocytic recycling pathway. Mammals express four paralogs of EHD proteins, EHD1-4, that are expressed in different tissues. Although EHD4 is expressed in the kidney, the specific physiological role of EHD4 in the kidney remains unknown. Therefore, this dissertation was focused to elucidate the physiological role of EHD4 in the kidney. In …

Mechanisms For The Potassium Sparing Effects Of Furosemide In Mice On High Potassium Diets, Bangchen Wang

Theses & Dissertations

Because of its cardio-protective benefits, diets that are low in Na⁺ and high in K⁺ are often warranted in conjunction with use of diuretics such as furosemide for treating hypertension. It is critical to understand how such diets influence the diuretic actions on renal K⁺ handling in order to choose the best drug for patients.

Furosemide is a commonly used K-wasting diuretic. It increases urinary K⁺ excretion by increasing distal Na⁺ delivery that stimulates K⁺ secretion. However, in wild-type mice (WT) adapted to an alkaline low Na⁺ high K⁺ diet (LNaHK), both …