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A Study Of The Effect Of Physiological Stressors On Hypothalamic Regulation Of Reproduction Using An In Vitro System, Lisa L. Amelse
A Study Of The Effect Of Physiological Stressors On Hypothalamic Regulation Of Reproduction Using An In Vitro System, Lisa L. Amelse
Doctoral Dissertations
Stressors have a negative impact on reproductive efficiency in humans and other animals, which has an economic cost due to infertility treatments for humans and reduced income for food producers. We wished to determine the molecular pathways by which metabolic disturbances and low-level inflammation impact the hypothalamic pituitary gonadal (HPG) axis using an in vitro model. To this end, we used the GT1-7 cell line, an immortalized line expressing the Kiss1R receptor that responds to kisspeptin stimulation with the release of GnRH to assess the impact of stressors on the GnRH-releasing cells. Additionally, we used the KTaV-3 and KTaR-1[A1] …