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Putative Pore-Lining Residues And Intracellular Magnesium Influence Connexin50 Unitary Gap Junction Channel Conductance, Swathy Sudhakar
Putative Pore-Lining Residues And Intracellular Magnesium Influence Connexin50 Unitary Gap Junction Channel Conductance, Swathy Sudhakar
Electronic Thesis and Dissertation Repository
Gap junction channels (GJs) are oligomers of connexins forming channels linking neighbouring cells. GJs formed by different connexins show distinct unitary channel conductance (γj), transjunctional-voltage dependent gating (Vj-gating) properties, and magnesium modulation. We investigate here how pore-lining residues may influence these GJ properties. We mutated putative pore-lining residues in Cx50 GJ (G8E/G46E/V53E individually or in combination) to investigate γj, Vj-gating, and magnesium modulation. GJs formed by combination mutants (G8E-G46E, G46E-V53E, and G8E-G46E-V53E) were functional and showed a significantly increased γj with little change in Vj-gating, while G8E-V53E mutants failed …