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Glycosylation On Stromal Interaction Molecule-1 Ca2+ Sensing Region Enhances Store Operated Ca2+ Entry, Yoo Jung Choi

Electronic Thesis and Dissertation Repository

A major intracellular calcium (Ca²⁺) uptake pathway in excitable and non-excitable eukaryotic cells is store-operated Ca²⁺ entry (SOCE). Stromal interaction molecule-1 (STIM1) is the key regulator of SOCE and responds to changes in endoplasmic reticulum (ER)-stored Ca²⁺ through luminal sensing machinery composed of EF-hand and SAM domains (EFSAM). EFSAM can undergo N-glycosylation at Asn131 and Asn171 sites; however, the exact molecular and functional effects of N-glycosylation are unclear. By establishing a site-specific chemical approach to covalently linking glucose to EFSAM and subsequently examining EFSAM biophysical properties, I found that this modification enhances STIM1 activation …