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Hypoxia-Induced Alterations In Skeletal Muscle Cell Respiration And Resveratrol As A Potential Pharmacological Intervention, Kathleen R. Belgrave

Electronic Thesis and Dissertation Repository

Using the C₂C₁₂ mouse muscle myoblast cell line, I sought to investigate the hypothesis that differentiation under hypoxia impairs muscle mitochondrial respiratory function irreversibly. Resveratrol (RSV) will increase expression of markers of mitochondrial biogenesis and reverse the hypoxia-induced depression of O₂ consumption rates. Hypoxia decreased oxygen consumption rates after five days of differentiation and after two days of normoxic recovery. This coincided with a hypoxia-induced decrease in protein levels of the NDUFB8 subunit of complex I of the electron transport chain and decreases in mRNA levels of mitochondrial biogenesis transcription factors, both of which could contribute …