Life Sciences Commons^™

Vitamin K2 (Menaquinone) Supplementation And Its Benefits In Cardiovascular Disease, Osteoporosis, And Cancer, Grant S. Buchanan, Md, Thomas Melvin, Brandon Merritt, Charles Bishop, Md, Franklin D. Shuler, Md, Phd

Franklin D. Shuler

Vitamin K is known to play an essential role in the coagulation cascade; however, a growing body of research has found that a subtype of this vitamin, vitamin K2 (menaquinone) may have a beneficial effect in osteoporosis, cardiovascular disease, and cancer. This purpose of this article is to provide a comprehensive review of recent literature regarding menaquinone and its role in human health. This review discusses the physiology of menaquinone, its clinical benefits in cardiovascular disease, osteoporosis, and cancer, and how it may interact with certain medications. The authors conclude that menaquinone supplementation has been shown to improve carboxylation of …

Spirometry Measurements During An Episode Of Exercise-Related Transient Abdominal Pain, Darren Morton

Darren Morton

Purpose: To determine whether changes in lung function are associated with exercise-related transient abdominal pain (ETAP). Methods: Twenty-eight subjects susceptible to ETAP performed a flow-volume loop before (pre) and after (post) treadmill exercise. Fourteen of the subjects developed symptoms of ETAP during the exercise and completed the flow-volume loop while the pain was present. The remaining 14 subjects reported no symptoms of ETAP. Results: Forced inspiratory vital capacity was essentially unchanged from pre to post in both groups (ETAP group –0.8% ± 5.1%, comparison group –0.9% ± 6.5%). Peak inspiratory-flow rate increased in both the ETAP group (12.4% ± 16.2%) …

Heart Rate Responses And Fluid Balance Of Competitive Cross-Country Hang Gliding Pilots, Darren Morton

Darren Morton

Purpose: To evaluate the physiological challenges of competitive cross-country hang gliding.

Methods: Seventeen experienced male pilots (age = 41 ± 9 y; mean ± SD) were fitted with a monitor that recorded heart rate and altitude at 0.5 Hz throughout a competitive flight. Fluid losses were evaluated by comparing pilot pre- and postflight mass.

Results: The pilots’ displacement was 88.4 ± 43.7 km in 145.5 ± 49.4 min. Mean flight altitude was 1902 ± 427 m (range = 1363–2601 m) with a maximum altitude of 2925 ± 682 m (1870–3831 m). The mean in-flight heart rate of the pilots was …

Strength Training For Plantar Fasciitis And The Intrinsic Foot Musculature: A Systematic Review., Dean Huffer, Wayne Hing, Richard Newton, Mike Clair

Wayne Hing

The aim was to critically evaluate the literature investigating strength training interventions in the treatment of plantar fasciitis and improving intrinsic foot musculature strength. A search of PubMed, CINHAL, Web of Science, SPORTSDiscus, EBSCO Academic Search Complete and PEDRO using the search terms plantar fasciitis, strength, strengthening, resistance training, intrinsic flexor foot, resistance training. Seven articles met the eligibility criteria. Methodological quality was assessed using the modified Downs and Black checklist. All articles showed moderate to high quality, however external validity was low. A comparison of the interventions highlights significant differences in strength training approaches to treating plantar fasciitis and …

The Physiological Profile Of Male Competitive And Recreational Surfers, James Furness, Wayne Hing, Jeremy M Sheppard, Sean Newcomer, Ben Schram, Mike Climstein

Ben Schram

Surfing consists of both high and low intensity paddling of varying durations, utilizing both the aerobic and anaerobic systems. Surf specific physiological studies lack adequate group sample sizes and VO2peak values are yet to determine differences between competitive and recreational surfers. The purpose of this study was therefore to provide a comprehensive physiological profile of both recreational and competitive surfers. This multi-site study involved 62 male surfers, recreational (n = 47) and competitive (n = 15). Anthropometric measurements were conducted followed by DEXA, anaerobic testing and finally aerobic testing. VO2peak was significantly greater in competitive compared to recreational surfers (M …

Clinical Methods To Quantify Trunk Mobility In An Elite Male Surfing Population, James Furness, Michael Climstein, Jeremy Sheppard, Allan Abbott, Wayne Hing

James Furness

Background

Thoracic mobility in the sagittal and horizontal planes are key requirements in the sport of surfing; however to date the normal values of these movements have not yet been quantified in a surfing population.

Objectives

To develop a reliable method to quantify thoracic mobility in the sagittal plane; to assess the reliability of an existing thoracic rotation method, and quantify thoracic mobility in an elite male surfing population.

Design

Clinical Measurement, reliability and comparative study.

Methods

A total of 30 subjects were used to determine the reliability component. 15 elite surfers were used as part of a comparative analysis …

Isothermal Titration Calorimetry Uncovers Substrate Promiscuity Of Bicupin Oxalate Oxidase From Ceriporiopsis Subvermispora, Hassan Rana, Patricia Moussatche, Lis Souza Rocha, Ellen W. Moomaw

Ellen Moomaw

Isothermal titration calorimetry (ITC) may be used to determine the kinetic parameters of enzymecatalyzed reactions when neither products nor reactants are spectrophotometrically visible and when the reaction products are unknown. We report here the use of the multiple injection method of ITC to characterize the catalytic properties of oxalate oxidase (OxOx) from Ceriporiopsis subvermispora (CsOxOx), a manganese dependent enzyme that catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction coupled with the formation of hydrogen peroxide. CsOxOx is the first bicupin enzyme identified that catalyzes this reaction. The multiple injection ITC method of measuring OxOx activity involves …

Isothermal Titration Calorimetry Uncovers Substrate Promiscuity Of Bicupin Oxalate Oxidase From Ceriporiopsis Subvermispora, Hassan Rana, Patricia Moussatche, Lis Souza Rocha, Ellen W. Moomaw

Ellen Moomaw

Isothermal titration calorimetry (ITC) may be used to determine the kinetic parameters of enzymecatalyzed reactions when neither products nor reactants are spectrophotometrically visible and when the reaction products are unknown. We report here the use of the multiple injection method of ITC to characterize the catalytic properties of oxalate oxidase (OxOx) from Ceriporiopsis subvermispora (CsOxOx), a manganese dependent enzyme that catalyzes the oxygen-dependent oxidation of oxalate to carbon dioxide in a reaction coupled with the formation of hydrogen peroxide. CsOxOx is the first bicupin enzyme identified that catalyzes this reaction. The multiple injection ITC method of measuring OxOx activity involves …

Exosomes Derived From Alcohol-Treated Hepatocytes Horizontally Transfer Liver Specific Mirna-122 And Sensitize Monocytes To Lps, Fatemeh Momen-Heravi, Shashi Bala, Karen Kodys, Gyongyi Szabo

Gyongyi Szabo

Hepatocyte damage and inflammation in monocytes/macrophages are central to the pathogenesis of alcoholic hepatitis (AH). MicroRNAs (miRNAs) regulate all of these processes. MiRNA-122 is abundantly expressed in hepatocytes while monocytes/macrophages have low levels. The role of exosomes in AH and possible cross talk between hepatocyte-derived exosomes and immune cells is not explored yet. Here, we show that the number of exosomes significantly increases in the sera of healthy individuals after alcohol binge drinking and in mice after binge or chronic alcohol consumption. Exosomes isolated from sera after alcohol consumption or from in vitro ethanol-treated hepatocytes contained miRNA-122. Exosomes derived from …

Na/K-Atpase Amplification Of Oxidant Stress; A Universal But Unrecognized Clinical Target?, Joseph I. Shapiro Md

Joseph I Shapiro MD

Schematic showing Na/K‐ATPase as reactive oxygen species (ROS) amplifier system. CTS‐cardiotonic steroids, Orange dimer is Na/K‐ATPase. pNaKtide refers to peptide formed from epitope of Na/K‐ATPase a1 subunit known to bind Src tyrosine kinase fused with TAT leader sequence.

Clinical Methods To Quantify Trunk Mobility In An Elite Male Surfing Population, James Furness, Michael Climstein, Jeremy Sheppard, Allan Abbott, Wayne Hing

Allan D. Abbott

Background

Thoracic mobility in the sagittal and horizontal planes are key requirements in the sport of surfing; however to date the normal values of these movements have not yet been quantified in a surfing population.

Objectives

To develop a reliable method to quantify thoracic mobility in the sagittal plane; to assess the reliability of an existing thoracic rotation method, and quantify thoracic mobility in an elite male surfing population.

Design

Clinical Measurement, reliability and comparative study.

Methods

A total of 30 subjects were used to determine the reliability component. 15 elite surfers were used as part of a comparative analysis …

Clinical Methods To Quantify Trunk Mobility In An Elite Male Surfing Population, James Furness, Michael Climstein, Jeremy Sheppard, Allan Abbott, Wayne Hing

Wayne Hing

Background Thoracic mobility in the sagittal and horizontal planes are key requirements in the sport of surfing; however to date the normal values of these movements have not yet been quantified in a surfing population. Objectives To develop a reliable method to quantify thoracic mobility in the sagittal plane; to assess the reliability of an existing thoracic rotation method, and quantify thoracic mobility in an elite male surfing population. Design Clinical Measurement, reliability and comparative study. Methods A total of 30 subjects were used to determine the reliability component. 15 elite surfers were used as part of a comparative analysis …

Using Mixed Methods To Measure The Perception Of Community Capacity In An Academic–Community Partnership For A Walking Intervention, Charkarra Anderson-Lewis, Diana Castellanos, Arnecca Byrd, Karen Zynda, Alicia Sample, Vickie Reed, Mary Beard, Latessa Minor, Kathleen Yadrick

Diana Cuy Castellanos

H.U.B. City Steps is a 5-year community-based participatory research walking intervention designed to help lower blood pressure in a majority African American population in southern Mississippi via community collaboration and capacity building, increased walking, culturally tailored health education sessions, and motivational interviewing. Building community capacity for physical activity is a key component of this intervention. Qualitative and quantitative methods have been used to assess how project stakeholders perceive the community capacity-building efforts of the project. This article illustrates the baseline results of this mixed methods approach from the perspective of three groups of stakeholders: project researchers and staff, community advisory …

Microrna Cargo Of Extracellular Vesicles From Alcohol-Exposed Monocytes Signals Naive Monocytes To Differentiate Into M2 Macrophages, Banishree Saha, Fatemeh Momen-Heravi, Karen Kodys, Gyongyi Szabo

Gyongyi Szabo

Membrane-coated extracellular vesicles (EVs) released by cells can serve as vehicles for delivery of biological materials and signals. Recently, we demonstrated that alcohol-treated hepatocytes cross-talk with immune cells via exosomes containing microRNA (miRNAs). Here, we hypothesized that alcohol-exposed monocytes can communicate with naive monocytes via EVs. We observed increased numbers of EVs, mostly exosomes, secreted by primary human monocytes and THP-1 monocytic cells in the presence of alcohol in a concentration- and time-dependent manner. EVs derived from alcohol-treated monocytes stimulated naive monocytes to polarize into M2 macrophages as indicated by increased surface expression of CD68 (macrophage marker), M2 markers (CD206 …

Interleukin 10 Restores Gastric Emptying, Electrical Activity, And Interstitial Cells Of Cajal Networks In Diabetic Mice, Anthony J. Bauer

Anthony Bauer

The Relationship Of Body Composition To Countermovement Vertical Jump Characteristics Across Loaded Conditions, C. J. Macdonald, H. S. Lamont, J. C. Smith, J. C. Cholewa, A. M. Suppe, Jeremy A. Gentles, L. D. Hurlbert

Jeremy A. Gentles

No abstract provided.