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Myocardial Alpha-Thrombin Receptor Activation Induces Hypertrophy And Increases Atrial Natriuretic Factor Gene Expression, C C. Glembotski, C E. Irons, K A. Krown, S F. Murray, A B. Sprenkle, C A. Sei
Myocardial Alpha-Thrombin Receptor Activation Induces Hypertrophy And Increases Atrial Natriuretic Factor Gene Expression, C C. Glembotski, C E. Irons, K A. Krown, S F. Murray, A B. Sprenkle, C A. Sei
Amy Sprenkle
The protease, a-thrombin (aTh), affects myocardial cell contractility, a feature common among agents that induce hypertrophy. However, it is not known whether cardiac myocytes possess aTh receptors (aTh-R), or if long term treatment with aTh can enhance growth and gene expression. In the present study primary neonatal rat ventricular myocytes expressed a 3.6-kilobase mRNA species that hybridized with a rat aTh-R-specific probe. After 48 h, aTh induced hypertrophy, sarcomeric organization, and enhanced atrial natriuretic factor (ANF) expression, all of which were blocked by the aTh-selective protease inhibitor, D-Phe-Pro-Argchloromethyl ketone. The aTh-R agonist peptide, SFLLRNPND, was a potent activator of ANF …
The Alpha-Adrenergic Stimulation Of Atrial Natriuretic Factor Expression In Cardiac Myocytes Requires Calcium Influx, Protein Kinase C, And Calmodulin-Regulated Pathways, C A. Sei, C E. Irons, A B. Sprenkle, P M. Mcdonough, J H. Brown, C C. Glembotski
The Alpha-Adrenergic Stimulation Of Atrial Natriuretic Factor Expression In Cardiac Myocytes Requires Calcium Influx, Protein Kinase C, And Calmodulin-Regulated Pathways, C A. Sei, C E. Irons, A B. Sprenkle, P M. Mcdonough, J H. Brown, C C. Glembotski
Amy Sprenkle
It has been shown recently that a-adrenergic agonists can stimulate atrial natriuretic factor (ANF) expression in ventricular cardiac myocytes; however, little is known about the intracellular signals mediating this activation. The present study focused on the potential roles of calcium-regulated kinases and calcium influx in the a-adrenergic stimulation of ANF gene expression in ventricular myocardial cell cultures. Myocardial cells maintained for 48 h in serumfree medium supplemented with phenylephrine (PE) possessed up to 15-fold higher levels of ANF peptide and ANF mRNA than control cells. The removal of PE, or the addition of nifedipine, resulted in a rapid decline in …