Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Bioanalytical spectroscopy (1)
- Biomarker (1)
- Boronic acid (1)
- CA125 (1)
- Carboxylic acids (1)
-
- Catechol (1)
- Chemical ecology (1)
- Dumetella carolinensis (1)
- Epitope (1)
- FTIR (1)
- Gas chromatographymass spectrometry (1)
- Gray catbird (1)
- Hammett correlation (1)
- Infrared spectroscopy (1)
- MUC16 (1)
- Melanin (1)
- Ovarian cancer (1)
- Repeat domain (1)
- Solid-phase microextraction (1)
- Static headspace sampling (1)
- Synthetic peptide (1)
- Uropygial gland (1)
- Volatile organic compounds (1)
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Synthesis And Structural Characterization Of The Peptide Epitope Of The Ovarian Cancer Biomarker Ca125 (Muc16), Rebecca J. Whelan, Zach T. Berman, Lee J. Moore, Kathleen E. Knudson
Synthesis And Structural Characterization Of The Peptide Epitope Of The Ovarian Cancer Biomarker Ca125 (Muc16), Rebecca J. Whelan, Zach T. Berman, Lee J. Moore, Kathleen E. Knudson
Faculty & Staff Scholarship
A highly conserved region of 21 amino acids flanked by cysteine residues, contained within a larger repeated domain, has been proposed to be the antibody-binding site in the ovarian cancer biomarker CA125 (MUC16). In this study solid-phase peptide synthesis with Fmoc protection chemistry was used to assemble a 21-mer peptide corresponding to the most frequently occurring antibody binding sequence in CA125. Potentially significant sequence variants were also synthesized. Peptide secondary structure was investigated using Fourier transform infrared spectroscopy, revealing the consensus sequence peptide to be largely unstructured at physiological pH whether the cysteine residues were reduced or were oxidized to …
Aryl Boronic Acid Inhibition Of Synthetic Melanin Polymerization, Jason M. Belitsky
Aryl Boronic Acid Inhibition Of Synthetic Melanin Polymerization, Jason M. Belitsky
Faculty & Staff Scholarship
Inhibitors of melanin formation are sought after for a range of applications. Boronophenylalanine is known to inhibit melanogenesis via boronic acid-catechol interactions. A spectroscopic assay was developed to study the polymerization of L-dopa to synthetic melanin in the presence of para-substituted aryl boronic acids. The best inhibition was observed for aryl boronic acids with electron-withdrawing substituents. The IC50 values exhibit a correlation with the Hammett sigma(p) parameter (rho = 0.97, r(2) = 0.92).
Short-Chain Carboxylic Acids From Gray Catbird (Dumetella Carolinensis) Uropygial Secretions Vary With Testosterone Levels And Photoperiod, Rebecca J. Whelan, Tera C. Levin, Mary C. Garvin
Short-Chain Carboxylic Acids From Gray Catbird (Dumetella Carolinensis) Uropygial Secretions Vary With Testosterone Levels And Photoperiod, Rebecca J. Whelan, Tera C. Levin, Mary C. Garvin
Faculty & Staff Scholarship
The uropygial gland of birds produces secretions that are important in maintaining the health and structural integrity of feathers. Non-volatile components of uropygial secretions are believed to serve a number of functions including waterproofing and conditioning the feathers. Volatile components have been characterized in fewer species, but are particularly interesting because of their potential importance in olfactory interactions within and across species. We used solid-phase microextraction headspace sampling with gas chromatography-mass spectrometry to detect and identify volatiles in uropygial secretions of gray catbirds (Dumetella carolinensis), a North American migratory bird. We consistently detected the following carboxylic acids: acetic, propanoic, 2-methylpropanoic, …