Life Sciences Commons^™

An Analysis Of Gulf Of Maine Cod Metapopulation Structure And Localized Stability, With Application To Area-Based Fisheries Management, Derek Olson

Electronic Theses and Dissertations

The complex metapopulation structure of groundfish stocks in the Gulf of Maine, particularly Atlantic cod (Gadus morhua), is largely ignored under the current system of broad-scale stock assessment and management. This mismatch of biological and administrative scales has contributed to severe overall stock decline and localized serial depletion of Gulf of Maine cod. Such chronic management failure has led to growing calls for a more area-based, finer-scale approach to managing groundfish in the Gulf of Maine. A primary difficulty hindering the development of finer-scale groundfish management has been the challenge of estimating fishing mortality upon local stock components. …

Roles Of Siphon Flows In Suspension Feeding, Kevin Du Clos

Electronic Theses and Dissertations

Benthic marine suspension feeders provide an important link between benthic and pelagic ecosystems. The strength of this link is determined by suspension-feeding rates. Many studies have measured suspension-feeding rates using indirect clearance-rate methods, which are based on the depletion of suspended particles. Direct methods that measure the flow of water itself are less common, but they can be more broadly applied because clearance-rate measurements are affected by properties of the cleared particles. We present pumping rates for three species of suspension feeders, the clams Mya arenaria and Mercenaria mercenaria and the tunicate Ciona intestinalis, calculated using a direct method …

Investigating High Speed Localization Microscopy Through Experimental Methods, Data Processing Methods, And Applications Of Localization Microscopy To Biological Questions, Andrew J. Nelson

Electronic Theses and Dissertations

Fluorescence Photoactivation Localization Microscopy(FPALM) and other super resolution localization microscopy techniques can resolve structures with nanoscale resolution. Unlike techniques of electron microscopy, they are also compatible with live cell and live animal studies, making FPALM and related techniques ideal for answering questions about the dynamic nature of molecular biology in living systems. Many processes in biology occur on rapid sub second time scales requiring the imaging technique to be capable of resolving these processes not just with a high enough spatial resolution, but with an appropriate temporal resolution. To that end, this Dissertation in part investigates high speed FPALM as …

Spreading Speeds Along Shifting Resource Gradients In Reaction-Diffusion Models And Lattice Differential Equations., Jin Shang

Electronic Theses and Dissertations

A reaction-diffusion model and a lattice differential equation are introduced to describe the persistence and spread of a species along a shifting habitat gradient. The species is assumed to grow everywhere in space and its growth rate is assumed to be monotone and positive along the habitat region. We show that the persistence and spreading dynamics of a species are dependent on the speed of the shifting edge of the favorable habitat, c, as well as c^*(∞) and c^*(−∞), which are formulated in terms of the dispersal kernel and species growth rates in both directions. When …

Social Ecological Food Systems: Sustainability Lessons From Maine Dairy Networks, Julia B. Mcguire

Electronic Theses and Dissertations

Milk production has played an integral role in the culture, landscape, and economy of Maine’s agriculture. Maine dairy farmers have faced numerous sustainability challenges to economic, environmental, and social aspects of their industry. Like many other complex social ecological systems, the Maine dairy industry faces a gap between scientific knowledge and actionable management or policy. A cultural dichotomy exists between conventional and organic farming. Shifting the focus from this binary, metrics such as social capital may play a key role in solving sustainability issues. Difficulties arise in the governance of complex social ecological systems when the scales of assessment, management, …

Size As A Trait For Understanding The Role Of Zooplankton In The Biological Carbon Pump, Karen Stamieszkin

Electronic Theses and Dissertations

Living organisms impact carbon transport between the atmosphere and the ocean through the biological carbon pump. Some plankton communities augment carbon export from the ocean’s surface, and are thought to have a major role in global climate. These export communities are often characterized by larger organisms that sink to depths where the carbon they contain is sequestered from the atmosphere. Zooplankton can enhance export by aggregating prey into larger sinking fecal pellets; however fecal pellet flux is a highly variable component of the biological carbon pump. Relating plankton trophic dynamics to changes in particulate carbon flux is an important step …

Shortnose Sturgeon (Acipenser Brevirostrum) Spawning Potential In The Penobscot River, Maine: Considering Dam Removals And Emerging Threats, Catherine Johnston

Electronic Theses and Dissertations

Dam removals from the Penobscot River in Maine restored access to freshwater habitat critical for the life cycle of endangered shortnose sturgeon. Prior to the dam removals, shortnose sturgeon spawning activity had not been documented. Instead, evidence suggested that individuals emigrated from the Penobscot River to spawn in the Kennebec complex, 140 km away. A central question of this thesis was whether spawning activity would commence in the first two years following dam removal. Consistent with pre-dam removal movement patterns determined using acoustic telemetry, the majority (78%) of tagged individuals emigrated from the Penobscot River at some point over the …

Exploring The Threats Of Dams And Ocean Conditions: In-River Movements And Ocean Growth Of Atlantic Salmon (Salmo Salar) From Maine's Rivers, Lisa K. Izzo

Electronic Theses and Dissertations

Substantial declines of anadromous Atlantic Salmon Salmo salar have occurred throughout the range of the species, with many populations at the southern extent of the distribution being extirpated or endangered. While Maine is the last state in the country where adult Atlantic Salmon return to rivers each year to spawn, numbers have decreased dramatically in recent decades, with typically less than 2,000 spawners returning to all Maine's rivers combined. The complex life history of this species, which involves a juvenile freshwater phase followed by a marine phase that can last one to five years before returning to freshwater to spawn …

Assessment Of A Hatchery Based Rainbow Smelt Supplementation Effort, Andrew O'Malley

Electronic Theses and Dissertations

Rainbow Smelt (Osmerus mordax) are an important fish distributed throughout northeastern North America with both anadromous and landlocked populations. Abundance, size at age, and maximum size vary widely among populations and life histories. In order to compare anadromous and landlocked populations, we collected spawning adults in 2014 from four anadromous and three landlocked populations. Scales and otoliths from the anadromous fish were examined and compared for estimates of bias and precision in ageing. Analysis of both scales and otoliths provided age estimates that were acceptable, but estimates from scales were more precise and had less bias. Otoliths were …

An Investigation Of Hydrogeologic, Stratigraphic, And Structural Controls On Acer Grandidentatum Communities In A Karst Landscape, Owl Mountain Province, Fort Hood Military Installation, Texas, Melinda S. Faulkner

Electronic Theses and Dissertations

The Owl Mountain Province is located within the Fort Hood Military Installation, an approximately 880 km2 installation established in the 1940s in Bell and Coryell counties, Texas, which has undergone extensive land use changes associated with military training, maintaining much of the vegetation in early succession. This study investigates thelithologic, stratigraphic, and structural controls on the hydrologic, hydrogeologic, and geomorphologic evolution of the Owl Mountain Province as expressed by mesic vegetation communities, including Pleistocene relicts Acer grandidentatum, within karst terrains. These systems exhibit complexly overprinted speleogenetic evolutions within a dynamic groundwater regime resulting from regional climate shifts throughout the …

Description Of Cranial Elements And Ontogenetic Change Within Tropidolaemus Wagleri (Serpentes: Crotalinae)., Nicolette L. Hill

Electronic Theses and Dissertations

Tropidolaemus wagleri is a species of Asian pitviper with a geographic range including Thailand, Vietnam, Malaysia, Singapore, Bruniei, parts of Indonesia, and the hilippines. Tropidolaemus is a member of the Crotalinae subfamily, within Viperidae. The genus Tropidolaemus includes five species, and was once included within the genus Trimeresurus. While some osteologic characteristics have been noted a comprehensive description of cranial elements has not been produced for T. wagleri. An in-depth description of the cranial skeleton of Tropidolaemus wagleri lays the foundation for future projects to compare and contrast other taxa within Crotalinae and Viperidae. The chosen reference specimen …

Analyzing The Phenologic Dynamics Of Kudzu (Pueraria Montana) Infestations Using Remote Sensing And The Normalized Difference Vegetation Index., Faye Peters

Electronic Theses and Dissertations

Non-native invasive species are one of the major threats to worldwide ecosystems. Kudzu (Pueraria montana) is a fast-growing vine native to Asia that has invaded regions in the United States making management of this species an important issue. Estimated normalized difference vegetation index (NDVI) values for the years 2000 to 2015 were calculated using data collected by Landsat and MODIS platforms for three infestation sites in Kentucky. The STARFM image-fusing algorithm was used to combine Landsat- and MODIS-derived NDVI into time series with a 30 m spatial resolution and 16 day temporal resolution. The fused time series was …

Integrated Analysis Of Mirna/Mrna Expression And Gene Methylation Using Sparse Canonical Correlation Analysis., Dake Yang

Electronic Theses and Dissertations

MicroRNAs (miRNAs) are a large number of small endogenous non-coding RNA molecules (18-25 nucleotides in length) which regulate expression of genes post-transcriptionally. While a variety of algorithms exist for determining the targets of miRNAs, they are generally based on sequence information and frequently produce lists consisting of thousands of genes. Canonical correlation analysis (CCA) is a multivariate statistical method that can be used to find linear relationships between two data sets, and here we apply CCA to find the linear combination of differentially expressed miRNAs and their corresponding target genes having maximal negative correlation. Due to the high dimensionality, sparse …

Towards Structural Determination Of Human Α1-Glycine Receptor Allostery, Rathna Jyothi Veeramachaneni

Electronic Theses and Dissertations

Recent advances in technology have led to the determination of numerous notable structures of membrane proteins. While they provide valuable information about the structure of membrane proteins these studies often provide static images with potentially limited dynamics, and structural determination often requires truncation of flexible regions, and often utilizes bacterial homologs given the need for stable, heterologous overexpression. In order to better understand allostery at a molecular level, state-dependent crosslinking studies coupled with multidimensional mass spectrometry (MS) were conducted on glycine receptor (GlyR) stabilized in different allosteric states. Predominant allosteric states were stabilized using wild type or mutated receptor in …

Current Status Of Falcon Populations In Saudi Arabia, Albara M. Binothman

Electronic Theses and Dissertations

Falcons (Falco spp.) are widely used for falconry in the countries of the Middle East. During the 2015 breeding season, we surveyed historic and active nest sites of Barbary (Falco pelegrinoides pelegrinoides) and Lanner (F. biarmicus) Falcons in Saudi Arabia. Field and questionnaire surveys were conducted and personal contact with falconers was made to document the current distribution and price changes for Lanner Falcons, Barbary Falcons, Saker Falcons (F. cherrug), Peregrine Falcons (F. peregrinus), Gyrfalcons (F. rusticolus), and Hybrid Falcons in Saudi Arabia. We categorized our survey into three geographic groups; southwest (A), northwest (B), and central (C) regions of …

Source Apportionment Of Atmospheric Particulate Matter In Developing Countries Using Trace Elements And Stable Metal Isotope Ratios, Nitika Dewan

Electronic Theses and Dissertations

The work presented herein details the source apportionment of atmospheric particulate matter in developing countries (Kyrgyzstan in Central Asia, metropolitan cities in Northern India, and Shenzhen in China) using trace elements and stable metal isotope ratios. The first study focused on the development of a novel method for the concomitant separation of rare-earth elements in environmental samples of any geological origin. The separation procedure is based on three extraction chromatographic materials, referred to as Sr.Spec, TRU.Spec, and Ln.Spec. This triple column arrangement enables the simultaneous isolation of pure Sr and Nd fractions in less than one day and with great …

C-Reactive Protein Interactions With Cellular Membranes And Supported Lipid Bilayers, Aml Abd Alhamed Alnaas

Electronic Theses and Dissertations

C-reactive protein (CRP) is a serum protein that binds to damaged membranes and initiates the complement immune response. Different forms of CRP are thought to alter how the body responds to inflammation and the degradation of foreign material. Despite knowing that a modified form of CRP(mCRP) binds to downstream protein binding partners better than the native pentameric form, the role of CRP conformation on lipid binding is yet unknown. In this work, three main assays were performed to characterize how conformation affects CRP-membrane interactions. The first assay utilized supported lipid bilayers that mimic the plasma membrane of apoptotic cells. The …

Ecology Of Largemouth Bass In An Aging Reservoir: Implications For Creating A Trophy Largemouth Bass Fishery, Jason Breeggemann

Electronic Theses and Dissertations

There are an estimated 3-9 million small reservoirs and nearly 1,000 large reservoirs in the United States. Most of these reservoirs were built several decades ago and are experiencing symptoms of reservoir aging, including loss of habitat, sedimentation, and decreased fishery production. Furthermore, over the last several decades, there has been an increasing interest among anglers for high quality, trophy fisheries. However, little is known about exactly how the reservoir aging process affects the ecology and especially growth potential of Largemouth Bass, particularly under potential climate change scenarios. Grand Lake in TX is a 45ha ageing reservoir that was built …

Functional Effects Of Calcium Regulation Of Thin Filaments At Single Particle Resolution, Christopher Solis-Ocampo

Electronic Theses and Dissertations

Heart disease is the leading cause of death in the United States. Understanding heart function at the molecular level is critical for developing of more effective treatments. In the cardiac muscle, the thin filament is composed by troponin (Tn), tropomyosin (Tm), and F-actin. It provides Ca2+-dependent regulation of contraction by modulating myosin attachment and force generation in a cooperative scheme. However, this mechanism remains unclear. To understand thin filament activation, we studied the binding and functional properties of Tn and Tm to F-actin at single particle resolution by employing fluorescence image colocalization, in vitro motility assays, and Förster resonance energy …

Financial Investment Management For Forest Sustainability, Zarina Ismailova

Electronic Theses and Dissertations

This is paper is discussion about main problems of forest management, whether financial investment has a substantial impact on the long term perspective of forest landscape restoration and, more specifically, what strategy and what financial options are available to make the forest projects more sustainable. A few relevant questions to ask are: who are the main actors in the implementation of FLR projects; which steps have to be taken; and, which financial options would more suitable and would be feasible to implement. There are three main parts of this research to be investigated:

1. forest issues along with their respective solutions, …

A Physiological Assessment Of Wetland Habitats For Spring-Migrating Ducks In The Agricultural Landscapes Of The Southern Prairie Pothole Region, Adam K. Janke

Electronic Theses and Dissertations

The conversion of grassland and wetland ecosystems in the Prairie Pothole Region (PPR) has been a pervasive challenge for conservationists dating back to the early 1900s. The legacy of ever-increasing agricultural intensity in the southern portions of the PPR, including eastern South Dakota, has left many wetland ecosystems in a matrix of intensive agricultural production. With little surrounding nesting cover, these wetlands are thought to have limited potential for waterfowl reproduction but may still play an important role facilitating migration of waterfowl en route to northern breeding areas during spring. My research sought to understand the contributions of wetlands in …

Changes In Wetland Conditions And Wetland Plant Communities In The Prairie Pothole Region After 50 Years, Ryann Cressey

Electronic Theses and Dissertations

Wetlands in Stutsman County, North Dakota were revisited after 50 years to assess changes in wetland conditions and plant communities within wetland zones in the Prairie Pothole Region. In 1961-1966, Robert E. Stewart and Harold A. Kantrud conducted a study to investigate the relationship of wetland plant communities to variations of water permanence and chemistry on three study areas: Crystal Springs, Cottonwood, and Mt. Moriah. Within in this region, a severe drought occurred in 1988- 1992 followed by the longest deluge starting in 1993 with wetlands still impacted today. In 2013 and 2014, I revisited 80 of the original wetlands …

Raptors In Temperate Grasslands: Ecology Of Ferruginous Hawk, Golden Eagle, And Northern Harrier In The Northern Great Plains, Shubham Datta

Electronic Theses and Dissertations

Ferruginous hawks (Buteo regalis) are a grassland and shrubland obligate nesting raptor and prefer lightly grazed pasture or idle areas for nesting. Their population reportedly declines in number if more than 30% of an area is cultivated and they rarely nest in areas dominated by croplands. Golden eagles (Aquila chrysaetos) are long-lived raptors with high nest-site fidelity and relatively low reproductive success. Population trends of golden eagles in western United States are unclear although long-term monitoring of populations shows declines in occupancy and breeding performance. Northern harriers (Circus cyaneus) prefer relatively open grasslands and …

Effect Of Oil And Natural Gas Development On Survival And Health Of White-Tailed Deer In The Western Dakotas, Katherine L. Moratz

Electronic Theses and Dissertations

Oil and gas development in North Dakota has resulted in the need for information regarding how increased activity has affected white-tailed deer (Odocoileus virginianus) populations. We evaluated white-tailed deer ecology in response to energy development and hypothesized that oil and gas development would negatively affect adult and neonate white-tailed deer due to increased vehicle traffic and human-related effects. We captured and radio-collared adult female and neonate white-tailed deer across three study areas: Dunn County, North Dakota, an area influenced by energy development, and Grant County, North Dakota, and Perkins County, South Dakota, areas not impacted by energy development at this …

Use Of Extraction Technologies In Food Safety Studies, Changling Qiu

Electronic Theses and Dissertations

Food safety has become a top concern in our society. The public in general is increasingly concerned about the safety of the food products they consume every day as more and more food contamination incidents and widespread recalls arise. It is necessary to trace any presence and/or the concentration levels of contaminants, pesticides, herbicides, or other harmful substances in food samples. Sample preparation is a crucial step in a food analytical method, as it takes up most of the total analysis time, contributing highly to the total cost of analysis and greatly influencing the results of the analysis. Traditional extraction …

Assessing Shoreline Change And Vegetation Cover Adjacent To Back-Barrier Shoreline Stabilization Structures In Georgia Estuaries, Katherine R. Wakefield

Electronic Theses and Dissertations

Anthropogenic stabilization of erosional shorelines by hard-armoring structures is used for protection of property, especially if buildings, historical monuments, cultural resources, or other infrastructure are present. The post-installation effects of shoreline stabilization structures on adjacent shorelines in the back-barrier marshes of coastal Georgia are a concern, and interest in living shorelines as erosion control devices has increased because of their use of natural materials and vegetation. AMBUR shoreline analysis software was used to calculate pre-and post-installation shoreline change rates of shorelines adjacent to riprap and bulkhead structures. There was no significant difference between the post-installation shoreline change rates of the …