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Full-Text Articles in Life Sciences
Methods For The Isolation Of Sensory And Primary Cilia—An Overview, Kimberly A. P. Mitchell, Gabor Szabo, Angela De S. Otero
Methods For The Isolation Of Sensory And Primary Cilia—An Overview, Kimberly A. P. Mitchell, Gabor Szabo, Angela De S. Otero
Faculty Publications and Presentations
Detailed proteomic analyses of mammalian olfactory and rod photoreceptor sensory cilia are now available, providing an inventory of resident ciliary proteins and laying the foundation for future studies of developmental and spatio-temporal changes in the composition of sensory cilia. Cilia purification methods that were elaborated and perfected over several decades were essential for these advances. In contrast, the proteome of primary cilia is yet to be established, because purification procedures for this organelle have been developed only recently. In this chapter we review current techniques for the purification of olfactory and photoreceptor cilia, and evaluate methods designed for the selective …
Ndp Kinase Moves Into Developing Primary Cilia, Kimberly A. P. Mitchell, Betty C. Gallagher, Gabor Szabo, Angela De S. Otero
Ndp Kinase Moves Into Developing Primary Cilia, Kimberly A. P. Mitchell, Betty C. Gallagher, Gabor Szabo, Angela De S. Otero
Faculty Publications and Presentations
Inmunofluorescence staining of murine NIH3T3 fibroblasts grown at high density shows that conventional nucleoside diphosphate (NDP) kinases A and B localize to a sensory organelle, the primary cilium. Similar results are obtained with Xenopus A6 kidney epithelial cells, suggesting that NDP kinases are a universal component of the primary cilium. The translocation of NDP kinase into primary cilia depends on size, taking place only when cilia reach a critical length of 5-6 μm. In mature cilia, NDP kinases are distributed along the ciliary shaft in a punctate pattern that is distinct from the continuous staining observed with acetylated α-tubulin, a …