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Full-Text Articles in Life Sciences
New Applications For The Kinetic Exclusion Assay (Kinexa), Mark Harrison Smith
New Applications For The Kinetic Exclusion Assay (Kinexa), Mark Harrison Smith
Boise State University Theses and Dissertations
This dissertation examines the fundamental principles and applicability of the kinetic exclusion assay (KinExA), developed and marketed by Sapidyne Instruments of Boise, Idaho, since 1995. Chapter One reviews and consolidates the manufacturer’s guidance and many early papers that delineate the practical and theoretical aspects of the technology. In brief, KinExA is a two stage analytical system. In stage one, a number of solutions are prepared, whereby one of the partners is kept constant (the constant binding partner, or CBP), while the other (the titrant) is varied, usually in serial dilution. As the titrant is increased, the free CBP decreases, and …
Kinetic Exclusion Assay Of Biomolecules By Aptamer Capture, Mark H. Smith, Daniel Fologea
Kinetic Exclusion Assay Of Biomolecules By Aptamer Capture, Mark H. Smith, Daniel Fologea
Physics Faculty Publications and Presentations
DNA aptamers are short nucleotide oligomers selected to bind a target ligand with affinity and specificity rivaling that of antibodies. These remarkable features recommend aptamers as candidates for analytical and therapeutic applications that traditionally use antibodies as biorecognition elements. Numerous traditional and emerging analytical techniques have been proposed and successfully implemented to utilize aptamers for sensing purposes. In this work, we exploited the analytical capabilities offered by the kinetic exclusion assay technology to measure the affinity of fluorescent aptamers for their thrombin target and quantify the concentration of analyte in solution. Standard binding curves constructed by using equilibrated mixtures of …