Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Synthesis And Evaluation Of Novel Silica Hydride-Based Stationary Phases For Bioanalytical Application, Seiichiro Watanabe
Synthesis And Evaluation Of Novel Silica Hydride-Based Stationary Phases For Bioanalytical Application, Seiichiro Watanabe
Seiichiro Watanabe
Most HPLC columns are packed with silanol-rich (Si-OH) type-B silica material that often participates in undesired electrostatic interactions with the analyte solutes and produces poor peak shape [1,2]. These silanols are also known to facilitate surface hydration necessary for HILIC retention mode for polar analyte molecules. However, the hydrated surface composition can easily fluctuate, and thus results in a poor reproducibility and requires lengthy equilibration step. In this study, four novel stationary phases have been developed by using the TYPE-CTM Silica Hydride material, which has replaced up to 95% of the surface silanols with silicon-hydride (Si-H) groups. One of the …