Life Sciences Commons^™

Effectiveness Of A Novel Spontaneous Carvacrol Nanoemulsion Against Salmonella Enterica Enteritidis And Escherichia Coli O157:H7 On Contaminated Mung Bean And Alfalfa Seeds, Kyle Landry, Yuhua Chang, David Julian Mcclements, Lynne Mclandsborough

Kyle S Landry

Pubertal And Adult Leydig Cell Function In Mullerian Inhibiting Substance-Deficient Mice, Xiufeng Wu, Ramamani Arumugam, Stephen Baker, Mary Lee

Mary M. Lee

Mullerian inhibiting substance (MIS) causes Mullerian duct regression during sexual differentiation and regulates postnatal Leydig cell development. MIS knockout (MIS-KO) mice with targeted deletions of MIS develop Leydig cell hyperplasia, but their circulating androgen concentrations are reportedly unaltered. We compared reproductive hormone profiles, androgen biosynthesis, and the expression of key steroidogenic and metabolic enzymes in MIS-KO and wild-type (WT) mice at puberty (36 d) and sexual maturity (60 d). In pubertal animals, basal testosterone and LH concentrations in plasma were lower in MIS-KO than WT mice, whereas human chorionic gonadotropin-stimulated testosterone concentrations were similar. In adults, basal LH, and both …

Characterization Of A Recently Purified Thermophilic Dnase From A Novel Thermophilic Fungus, Kyle Landry, Robert Levin

Kyle S Landry

The Orientation Of Strophomenid Brachiopods On Soft Substrates, Roy Plotnick, Benjamin Dattilo, Daniel Piquard, Jennifer Bauer, Joshua Corrie

Benjamin F. Dattilo

Strophomenid brachiopods have long been interpreted as ‘‘snowshoe’’ strategists, with their flattened concavoconvex valves providing resistance to foundering in very soft sediments. There has been a sharp difference of opinion in whether the shells were oriented with their convex or their concave surface in contact with the sediment. This study, along with independent evidence from sedimentology, ichnology, and morphology, indicates that the strophomenids lived with their shells concave down (convex up). Experiments indicate the force required to push shells into soft cohesive muds is much greater for the convex up than for the convex down orientation. Forces also increase with …

Crystal Structure And Functional Assignment Of Yfau, A Metal Ion Dependent Class Ii Aldolase From Escherichia Coli K12, Dean Rea, Rebecca Hovington, John Rakus, John Gerlt, Vilmos Fu¨Lo¨P, Timothy Bugg, David Roper

John F. Rakus

One of the major challenges in the postgenomic era is the functional assignment of proteins using sequence- and structure-based predictive methods coupled with experimental validation. We have used these approaches to investigate the structure and function of theEscherichia coli K-12 protein YfaU, annotated as a putative 4-hydroxy-2-ketoheptane-1,7-dioate aldolase (HpcH) in the sequence databases. HpcH is the final enzyme in the degradation pathway of the aromatic compound homoprotocatechuate. We have determined the crystal structure of apo-YfaU and the Mg2+−pyruvate product complex. Despite greater sequence and structural similarity to HpcH, genomic context suggests YfaU is instead a 2-keto-3-deoxy sugar aldolase like the …

Stratigraphic Distribution And Suggested Evolution Of Dendroid Graptolites From The Silurian Of Eastern Australia, Barrie Rickards, Anthony Wright

Anthony Wright

Five evolutionary lineages are proposed for Silurian species of the benthic dendroid graptolite genus Dictyonema, based largely on the exceptional eastern Australian records of the genus, comprising at least 25 species. These are: A, the delicatulum lineage with bifurcating ventral autothecal apertural spines; B, the paululum lineage with single ventral apertural spines or processes; C, the elegans lineage with isolated thecal apertures ± processes; D, the sherrardae lineage with dorsal apertural processes; and E, the venustum lineage with simple autothecal apertures. Brief comments are also made on other dendroid genera occurring in Australian strata, namely: Acanthograptus, Koremagraptus, Callograptus, Dendrograptus, Stelechocladia, …

Development Of A Novel Affinity Membrane Purification System For Deoxyribonuclease, Kyle Landry, Robert Levin

Kyle S Landry

The Evolution Of Billfish, Andrew Blitman

Andrew Blitman

No abstract provided.

Insights Into The Cation Permeation Pathway Of Channelrhodopsin-2, Robert Dempski, Ryan Richards

Robert E. Dempski

Channelrhodopsin-2 (ChR2) is a light-activated, non-selective cation channel endogenous to the green algae Chlamydomonas reinhardtii. The unique properties of ChR2 have made it a useful tool in the field of optogenetics. However, the mechanism of ion conductance is not well resolved. Elucidation of the crystal structure of the channelrhodopsin chimera C1C2 has provided structural insight on the putative ChR2 ion conductance pathway. However, it is not clear how the chimeric structure correlates to ChR2 function.

Purification Of An Inducible Dnase From A Thermophilic Fungus, Kyle Landry, Andrea Vu, Robert Levin

Kyle S Landry

Purification And Characterization Of Iso-Ribonucleases From A Novel Thermophilic Fungus, Kyle Landry, Robert Levin

Kyle S Landry