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Antiviral Activity Of Four Types Of Bioflavonoid Against Dengue Virus Type-2, Wong Pooi Fong
Antiviral Activity Of Four Types Of Bioflavonoid Against Dengue Virus Type-2, Wong Pooi Fong
Wong Pooi Fong
Background: Dengue is a major mosquito-borne disease currently with no effective antiviral or vaccine available. Effort to find antivirals for it has focused on bioflavonoids, a plant-derived polyphenolic compounds with many potential health benefits. In the present study, antiviral activity of four types of bioflavonoid against dengue virus type -2 (DENV-2) in Vero cell was evaluated. Anti-dengue activity of these compounds was determined at different stages of DENV-2 infection and replication cycle. DENV replication was measured by Foci Forming Unit Reduction Assay (FFURA) and quantitative RT-PCR. Selectivity Index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC50) …
In Vitro Antiviral Activity Of Fisetin, Rutin And Naringenin Against Dengue Virus Type-2, Wong Pooi Fong
In Vitro Antiviral Activity Of Fisetin, Rutin And Naringenin Against Dengue Virus Type-2, Wong Pooi Fong
Wong Pooi Fong
In vitro antiviral activities of three flavonoids; fisetin, naringenin and rutin against DENV-2 (NGC strain) were evaluated. Inhibitory effects of each compound at the different stages of DENV-2 infection were examined using foci forming unit reduction assay (FFURA) and quantitative real-time polymerase chain amplification (qRT-PCR). Fisetin, rutin and naringenin showed cytotoxic effects against Vero cells with 50% cytotoxicity (CC(50)) values of 247, > 1000, and 87 mu g/mL, respectively. Fisetin when added to Vero cells after virus adsorption inhibited DENV replication with a half maximal inhibition concentration (IC(50)) value of 55 mu g/mL and selectivity index (SI) of 4.49. The IC(50) …