Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Department of Pharmacology and Toxicology (97)
- Pharmacology (30)
- Toxicology (17)
- ACE2 (6)
- Diabetes (6)
-
- Sarin (5)
- TCDD (5)
- Cytokines (4)
- Keratinocytes (4)
- Medicine (4)
- Molecular Biology (4)
- RAS (4)
- Biomedical Research (3)
- Collagen (3)
- Growth Factors (3)
- Hydroxyurea (3)
- IGF-1 (3)
- Mass Spectrometry (3)
- Microvesicle Particles (3)
- Organophosphate (3)
- Platelet Activating Factor (3)
- Silver Nanoparticles (3)
- Skin cancer (3)
- Stroke (3)
- 8-OH-DPAT (2)
- ACE (2)
- ATR inhibitor (2)
- ATR kinase (2)
- Acid Sphingomyelinase (2)
- Acid sphingomyelinase (2)
- Publication Year
- Publication
- Publication Type
Articles 1 - 30 of 109
Full-Text Articles in Life Sciences
Toxicity Of Three Species Of The Solanaceae Family Growing In Algeria Against Culiseta Longiareolata Mosquitos 4th Stage Larvae, Saliha Benhissen, Abdelmaddjid Yagoub Asloum, Nora Belkhiri, Zakaria Hedjouli, Siham Bounadji, Wafa Habbachi, Khellaf Rebbas
Toxicity Of Three Species Of The Solanaceae Family Growing In Algeria Against Culiseta Longiareolata Mosquitos 4th Stage Larvae, Saliha Benhissen, Abdelmaddjid Yagoub Asloum, Nora Belkhiri, Zakaria Hedjouli, Siham Bounadji, Wafa Habbachi, Khellaf Rebbas
Journal of Bioresource Management
Mosquitoes have always been considered a source of harm to humans and animals mainly because they can be vectors of disease. This work represents a study on the effect of aqueous extract of three plants (Hyoscyamus albus, Solanum elaeagnifolium, Solanum nigrum) of the Solanaceae family on the mortality of 4th stage larvae of Culiseta longiareolata larvae. Extract of the high impact is of S. nigrum followed by H. albus and S. elaeagnifolium, respectivley of C. longiareolata larvae.
Role Of Urban Parks In Carbon Sequestration– A Case Study Of Safari Park, Karachi, Pakistan, Amna Bano, Minzah Shehzad, Hasnain Kazmi, Jamshaid Iqbal
Role Of Urban Parks In Carbon Sequestration– A Case Study Of Safari Park, Karachi, Pakistan, Amna Bano, Minzah Shehzad, Hasnain Kazmi, Jamshaid Iqbal
Journal of Bioresource Management
Urban parks besides their recreational use can be the potential source of climate mitigation through carbon sequestration. Present study aims to identify the carbon sequestration potential of Safari Park which is by far the largest public park of Karachi established in 1970 covering an area of 0.72 km2. A total of 153 individual trees belonging to 25 species and 14 families were included in the study. Five dominant species with highest Important Value Index (IVI) were Cocos nucifera (14.62 %), Azadirachta indica (14.21 %), Guaiacum officinale (9.93 %), Washington robusta (9.31 %) and Delonix regia (7.11 %). The …
Ethnobotanical Study Of Antihemorrhagic Plants In The Skikda Region (North East Of Algeria), Nabila Souilah, Hamdi Bendif, Abdelkrim Benaradj, Abdelouaheb Belaid, Scherazad Mekious
Ethnobotanical Study Of Antihemorrhagic Plants In The Skikda Region (North East Of Algeria), Nabila Souilah, Hamdi Bendif, Abdelkrim Benaradj, Abdelouaheb Belaid, Scherazad Mekious
Journal of Bioresource Management
Our survey was carried out at the level of 17 Municipalities of the Province of Skikda. Questionnaire sheets were distributed to 55 herbalists and 350 locale population (informants). The processing of survey data with the local population shows us that women (52 %) have more knowledge of phytotherapy compared to men (48 %) with a fairly high level of education. For the herbalists, we found that most are men (85.45 %) aged between 31-40 years (29.09 %) and 41-50 years (25.45 %), and with level of university study (36.36 %). On the other hand, 52.73 % of herbalists said they …
Human Health Risk Assessment Of Some Heavy Metals In Eme River, Umuahia, Abia State, Nigeria, Emeka Donald Anyanwu, Onyinyechi Gladys Adetunji, Paschal Emeka Etusim, Solomon Nnanna Umeham
Human Health Risk Assessment Of Some Heavy Metals In Eme River, Umuahia, Abia State, Nigeria, Emeka Donald Anyanwu, Onyinyechi Gladys Adetunji, Paschal Emeka Etusim, Solomon Nnanna Umeham
Journal of Bioresource Management
This study evaluated the health risk of some heavy metals in Eme River, Umuahia. The study conducted in 6 stations was between December 2017 and November 2018. The non-carcinogenic method that involves determination of chronic daily intake (CDI), hazard quotient (HQ) and hazard index (HI) was applied in the assessment of ingestion, dermal and incidental ingestion exposures. Iron, lead and cadmium exceeded their limits and warranted risk assessment. The heavy metals’ CDI values for the three routes were lower than their respective reference doses except for cadmium (ingestion route). Except for cadmium (ingestion), the hazard quotients also recorded values lower …
Morphological And Physiological Responses Of A Halophyte (Atriplex Halimus) To The Effect Of Heavy Metal Case Of Cadmium, Abderrezzeq Chebout, Hana Souahi, Zahia Kadi, Rania Gacem
Morphological And Physiological Responses Of A Halophyte (Atriplex Halimus) To The Effect Of Heavy Metal Case Of Cadmium, Abderrezzeq Chebout, Hana Souahi, Zahia Kadi, Rania Gacem
Journal of Bioresource Management
Today, cadmium (Cd) contamination challenges the environmental quality and food security. This experiment was realized to study the morphological and physiological response of the halophyte species Atriplex halimus L to cadmium toxicity by applying different concentrations of Cd (0, 500, 1000, 1500, 2000 ppm) on the plant A.halimus for two weeks after 60 days of seeding. The morphological parameters and physiological wish evaluated the stem length (SL), root length (RL), leaf area (LA), chlorophylls (a, b, t and carotenoids), and the relative water content (RWC). The results show a decrease in stem elongation, (11.333 ± 3.512 cm in Cd treatments …
Potential Of Constructed Wetlands For The Treatment Of Heavy Metals From Wastewater, Hasnain Raza, Qurat Ul Ain, Haseena Bibi, Tehmina Bibi, Huda Bilal
Potential Of Constructed Wetlands For The Treatment Of Heavy Metals From Wastewater, Hasnain Raza, Qurat Ul Ain, Haseena Bibi, Tehmina Bibi, Huda Bilal
Journal of Bioresource Management
Overpopulation, urbanization, and economic race increase the pressure on freshwater resources resulted in water scarcity challenges around the globe. Moreover, the generation of wastewater also enhancing the scarcity threats for freshwater resources. Water scarcity issues forced the policymaker, thinker, scientist, and researcher to think about non-conventional water resources to cope with the increasing demands. Because of this wastewater recycling and treatment play an evident role to fulfill the growing demand for freshwater in socioeconomic sectors especially, for agriculture due to its 70 % dependency on freshwater resources. Different wastewater treatment methods along with merits and demerits are in practice for …
Heightened Levels Of Microvesicle Particles Resulting From Combination Of Ethanol And Thermal Burn Injury, Chad Alan Brewer
Heightened Levels Of Microvesicle Particles Resulting From Combination Of Ethanol And Thermal Burn Injury, Chad Alan Brewer
Browse all Theses and Dissertations
Ethanol (EtOH), in combination with thermal burn injury, is a clinically significant problem resulting in an increase in morbidity and mortality due to acute multi-organ toxicity from excess systemic cytokine release. This intoxicated thermal burn affects close to 50% of the total numbers of hospital-admitted burn patients and currently has no standard treatment. However, using in vitro cellular and in vivo murine models, our group has provided data implicating the augmented production of the lipid mediator Platelet-activating factor (PAF) in keratinocytes in response to intoxicated thermal burn injury in the subsequent pathology. Of importance, our group has demonstrated that activation …
Replication Stress Induced By The Ribonucleotide Reductase Inhibitors Guanazole, Triapine, And Gemcitabine In Fission Yeast, Mashael Yahya A Alyahya
Replication Stress Induced By The Ribonucleotide Reductase Inhibitors Guanazole, Triapine, And Gemcitabine In Fission Yeast, Mashael Yahya A Alyahya
Browse all Theses and Dissertations
Replication stress can be produced by various exogenous or endogenous factors that perturb the movement of replication forks. To overcome the stress, the cells provoke the DNA replication checkpoint to mobilize several pathways to protect the forks, maintain genome integrity, and promote cell survival. Defects in the replication checkpoint cause forks collapse, leading to chromosomal DNA damage or cell death. Although the replication checkpoint is crucial for genome integrity in all eukaryotes, the underlying mechanisms remains to be fully understood. To investigate the mechanisms of DRC, hydroxyurea (HU), an established inhibitor of ribonucleotide reductase (RNR), has been widely used in …
Impaired Β-Cell Neogenesis In A Mouse Model Of Metabolic Syndrome, Modhi Abdullah Alshammari
Impaired Β-Cell Neogenesis In A Mouse Model Of Metabolic Syndrome, Modhi Abdullah Alshammari
Browse all Theses and Dissertations
Pancreatic islet β-cell plays an essential role in insulin release and hence glucose homeostasis. The maintenance of glucose homeostasis depends on β-cell ability to cope with enough insulin to fulfill metabolic and physiological demands. Adequate insulin release is the result of highly complex and dynamic interplays between acute changes in β- cell electrical activity, exocytosis and chronic adaptations in cellular function, volume, mass and proliferation. All of this appears modulated, to some extent, by the functional presence of Slc12a2 symporters, also known as Na+K+2Cl– cotransporter-1 (Nkcc1), which accumulates intracellular Cl– above its electrochemical equilibrium. Recent studies from our laboratory showed …
Ahr-Mediated Transcriptional Regulation Of The Human Immunoglobulin Hs1.2 Enhancer, Sydney White
Ahr-Mediated Transcriptional Regulation Of The Human Immunoglobulin Hs1.2 Enhancer, Sydney White
Browse all Theses and Dissertations
The 3'IGHRR is thought to be responsible for the transcription of the immunoglobulin heavy chain (IGH) gene locus, which is essential for the production of antibodies. The human 3’IGHRR, which is duplicated in humans, contains the hs3, hs4, and hs1.2 enhancers. Additionally, the hs1.2 enhancer is polymorphic in humans and consists of a 53 bp invariant sequence that can be repeated one to four times. Previous experiments in a mouse and human B-cell line model have shown that the high affinity AhR ligand and environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) can induce hs1.2 enhancer activity in an AhR-dependent manner. The AhR is …
Plastic Biodegradation Through Insects And Their Symbionts Microbes: A Review, Huda Bilal, Hasnain Raza, Haseena Bibi, Tehmina Bibi
Plastic Biodegradation Through Insects And Their Symbionts Microbes: A Review, Huda Bilal, Hasnain Raza, Haseena Bibi, Tehmina Bibi
Journal of Bioresource Management
Plastic waste has recently been identified as one of the most serious environmental issues, affecting all life forms, natural habitats, and the economy, and is one of the most serious global environmental problems, second only to climate change. Seeking alternative environmentally sustainable options, such as biodegradation instead of conventional disposal, is critical in the face of this challenge. However, there is currently a lack of information about the mechanisms and efficacy of plastic biodegradation. From this perspective, this study aims to illustrate the negative environmental impacts of the plastic waste. It also addresses the role of insects and gut microbiota …
The Effect Of Circadian Clock Modulation On Cisplatin Cytotoxicity, Nadeen Nibal Ahmad Anabtawi
The Effect Of Circadian Clock Modulation On Cisplatin Cytotoxicity, Nadeen Nibal Ahmad Anabtawi
Browse all Theses and Dissertations
Cisplatin is a DNA damage-based chemotherapeutic drug widely used to treat various types of cancers; however, the treatment's toxicity restricts its efficiency. Studies have shown that the circadian rhythm controls the DNA damage response and affects the repair pathways of cisplatin-induced DNA damage. Circadian clock modulation, therefore, has been proposed to be a potential mechanism for enhancing cisplatin tolerability. Here we used clock-enhancing molecules to evaluate the effect of pharmacological clock modulation on cisplatin cytotoxicity. Using cultured human cell lines, cisplatin cytotoxicity was found to be attenuated following treatment with circadian-enhancing molecules KS15 and SR8278. Moreover, the protein and mRNA …
Stress-Induced Senescence In Human Dermal Fibroblasts : Effects Of Creatine And Nicotinamide Post Stress Treatment, Venkata Sravya Arikatla
Stress-Induced Senescence In Human Dermal Fibroblasts : Effects Of Creatine And Nicotinamide Post Stress Treatment, Venkata Sravya Arikatla
Browse all Theses and Dissertations
In skin connective tissue, dermal fibroblasts appear to be the most prevalent cell type. They are in charge of making the extracellular matrix that makes up the skin's connective tissue, and also involved in wound healing. Moreover, they produce Insulin-like growth factor-1 (IGF-1) which helps in activation of Insulin growth factor-1 receptor (IGF-1R). This receptor helps to control cell proliferation and responses to DNA-damaging substances such as UVB radiation, reactive oxygen species (ROS), and therapeutic drugs. According to our findings, lack of IGF-1 expression in the dermis of elderly patients due to fibroblast senescence (senescence is characterized by which cells …
The Protective Effects Of Mir-210 Modified Endothelial Progenitor Cells Released Exosomes In Hypoxia/Reoxygenation Injured Neurons, Sri Meghana Yerrapragada
The Protective Effects Of Mir-210 Modified Endothelial Progenitor Cells Released Exosomes In Hypoxia/Reoxygenation Injured Neurons, Sri Meghana Yerrapragada
Browse all Theses and Dissertations
Endothelial progenitor cells (EPCs) have been shown to provide beneficial effects on oxidative stress. Exosomes (EXs) released from these stem cells could be one of the major contributors, as they are known to convey the benefit of one cell to another cell via microRNAs (miRNA). At first, we determined that EPCs release more EXs when they are serum-starved for 48 hours., and by determining the microRNA-210 (miR-210) levels in the EXs, we found that miRNA is being transferred from cells to EXs. Meanwhile, miR-210 is gaining popularity in reducing elevated oxidative stress levels. In this study, we investigated the role …
Functions Of Atr Kinase In Terminally Differentiated Human Epidermal Keratinocyles And In Human Ex-Vivo Skin After Exposure To Ultraviolet B Radiation, Vivek Shashank Nag Gogusetti
Functions Of Atr Kinase In Terminally Differentiated Human Epidermal Keratinocyles And In Human Ex-Vivo Skin After Exposure To Ultraviolet B Radiation, Vivek Shashank Nag Gogusetti
Browse all Theses and Dissertations
The functions of Ataxia telangiectasia and Rad-3 related protein (ATR) is very much important in a cell, as it is a DNA damage response protein, which plays an important role in cell division, DNA repair and apoptosis. This protein helps in proliferation in the actively DNA dividing normal cells and in cancer cells. The functions of ATR in a proliferating cell are well studied and known to involve regulation of replication fork and cell cycle progression after DNA damage. Whereas, in a non-replicating cell, the functions of ATR are not so well known. In the human body, most of the …
Xeroderma Pigmentosum A Deficiency Results In Increased Generation Of Microvesicle Particles In Response To Ultraviolet B Radiation, Christian Lea Rajeshkumar
Xeroderma Pigmentosum A Deficiency Results In Increased Generation Of Microvesicle Particles In Response To Ultraviolet B Radiation, Christian Lea Rajeshkumar
Browse all Theses and Dissertations
Xeroderma Pigmentosum is a genetic disorder in which ability to repair DNA damage such as from UV radiation is decreased. Nucleotide excision repair is known for repairing DNA damage caused by UV radiation and XPA plays a major role in recognizing and eliminating abnormal section of DNA. Therefore, XPA deficiency decreases repair efficiency of DNA. Of note, XPA deficiency is linked with photosensitivity. Microvesicle particles are membrane-bound particles which are released into the extracellular environment in response to multiple stimuli including the lipid Platelet activating factor (PAF). Previous studies have shown that XPA deficiency can induce increase production of reactive …
Prevalence Of Tuberculosis In District Haveli, Azad Jammu And Kashmir, Abu Ul Hassan Faiz, Ghazal Tariq, Ayesha Faiz, Lariab Zahra Faiz
Prevalence Of Tuberculosis In District Haveli, Azad Jammu And Kashmir, Abu Ul Hassan Faiz, Ghazal Tariq, Ayesha Faiz, Lariab Zahra Faiz
Journal of Bioresource Management
Tuberculosis is an infectious disease with a common occurrence in population of Azad Jammu and Kashmir. The current study was designed to find out the prevalence of TB among the general population of a less developed District Haveli. Samples were collected from different hospitals and TB centers located in district Haveli and tested through sputum smear microscopy for conformation of positive for TB. The three-year (2015 to 2018) study was conducted to record prevalence of tuberculosis in selected area of Azad Jammu and Kashmir. The trend line of prevalence was made by using (SPSS software). Results produced linear trends yearly …
Land Application Of Sewage Sludge: Physiological And Biochemical Response Of The Rio Grande Tomato, Nabil Charchar, Ali Elafri, Redwane Rais, Halassi Ismahen
Land Application Of Sewage Sludge: Physiological And Biochemical Response Of The Rio Grande Tomato, Nabil Charchar, Ali Elafri, Redwane Rais, Halassi Ismahen
Journal of Bioresource Management
Treated wastewater produces sewage sludge as a by-product that may have beneficial implications on agricultural practices. The effects of sludge amendments on growth, morphological and biochemical characteristics of the agro-industrial Tomato cultivar Rio Grande were observed. A pot culture experiment was carried out during 4 months (16 February 2019 to 18 June 2019), at an experimental green house in the locality of Salah Soufi, Guelma, north-eastern Algeria. There were significant differences between soil and sewage sludge samples. Total nitrogen (20.58 mg/kg) was more available in sewage sludge than in soil (N = 2.09 ± 0.3 mg/kg). In contrast, sludge …
Tip60 Regulation Of Δnp63Α Is Associated With Cisplatin Resistance, Akshay Hira, Andrew Stacy, Jin Zhang, Michael P. Craig, Madhavi Kadakia
Tip60 Regulation Of Δnp63Α Is Associated With Cisplatin Resistance, Akshay Hira, Andrew Stacy, Jin Zhang, Michael P. Craig, Madhavi Kadakia
Symposium of Student Research, Scholarship, and Creative Activities Materials
About 5.4 million basal and squamous cell skin cancers are diagnosed every year in the US. ΔNp63a, a member of the p53 transcription factor family, is overexpressed in non-melanoma skin cancer and regulates cell survival, migration and invasion. TIP60 is histone acetyltransferase (HAT) which mediates cellular processes such as transcription and the DNA damage response (DDR). Previous studies in our lab have shown that overexpression of TIP60 induces ΔNp63a protein stabilization in a catalytic-dependent manner. Since ΔNp63a is known to transcriptionally regulate several DDR genes and promote cisplatin resistance, its stabilization by TIP60 may contribute to the failure of platinum-based …
Multi-Label Model For Toxicity Prediction, Xiu Huan Yap, Michael L. Raymer
Multi-Label Model For Toxicity Prediction, Xiu Huan Yap, Michael L. Raymer
Symposium of Student Research, Scholarship, and Creative Activities Materials
Most computational predictive models are specifically trained for a single toxicity endpoint. Since more than 1300 toxicity assays have been reported in the TOXCAST dashboard, achieving high coverage over this growing number of toxicity endpoints remains challenging. Furthermore, single-endpoint models lack the ability to learn dependencies between endpoints, such as those targeting similar biological pathways, which may be used to boost model performance. In this study, we characterize the performance of 3 multi-label classification (MLC) models, namely Classifier Chains (CC), Label Powersets (LP) and Stacking (SBR), on Tox21 challenge data. These MLC models employ the Problem Transformation approach, which is …
Insulin-Like Growth Factor-1 (Igf-1) Impacts P53-Regulated Gene Products In Uvb-Irradiated Human Keratinocytes And Skin Epidermis, Abdulrhaman Mohammed Mohammed Alkawwar
Insulin-Like Growth Factor-1 (Igf-1) Impacts P53-Regulated Gene Products In Uvb-Irradiated Human Keratinocytes And Skin Epidermis, Abdulrhaman Mohammed Mohammed Alkawwar
Browse all Theses and Dissertations
Skin cancer is the most prevalent human malignancy and is primarily caused by ultraviolet (UV) wavelengths of sunlight. However, the fact that most skin cancers occur in people over the age of 60 indicates that advantaged age is a second skin cancer risk factor. Why geriatric skin is prone to developing skin cancers is not clear, but several studies have shown that dermal fibroblasts in geriatric skin express lower levels of the hormone insulin-like growth factor-1 (IGF-1) than young adult skin and that deficient IGF-1 signaling negatively impacts how epidermal keratinocytes respond to UVB radiation. A major regulator of the …
Identification Of New Metabolic Mutations In The Fission Yeast Schizosaccharomyces Pombe That Sensitize The Cell To Hydroxyurea, Alaa Mahdi
Browse all Theses and Dissertations
Cancer is a life-threatening illness and innovative research is therefore required to fuel the development of new anti-cancer therapies. As an anti-proliferative drug, hydroxyurea (HU) has been used in the treatment of various neoplastic and non-neoplastic diseases such as sickle cell anemia, psoriasis, and viral infections. HU is a well-known inhibitor of ribonucleotide reductase (RNR), an enzyme that generates dNTPs for DNA replication and repair. In our genetic screen in fission yeast looking for mutants with defects in checkpoint response, we also found a set of mutants that are highly sensitive to HU but with a functional checkpoint response. It …
Multiple Genotoxic Agents Activate Atr Kinase Signaling In Quiescent Human Cells, Mariyyah Ahmed O. Madkhali
Multiple Genotoxic Agents Activate Atr Kinase Signaling In Quiescent Human Cells, Mariyyah Ahmed O. Madkhali
Browse all Theses and Dissertations
The ATR protein kinase is activated in response to DNA damage and other forms of genotoxic stress caused by both environmental carcinogens and anti-cancer drugs. However, much of our understanding of ATR kinase function is limited to proliferating cells in which DNA replication stress is the primary signal for ATR activation and where the major regulatory targets of ATR signaling are proteins involved in DNA synthesis and cell cycle progression. Here we have used HaCaT keratinocytes maintained in a non-replicating, quiescent state in vitro to examine how cell killing by different genotoxic agents is impacted by cell growth status and …
Topical Photodynamic Therapy Generates Microvesicle Particles, Oladayo Ayobami Oyebanji
Topical Photodynamic Therapy Generates Microvesicle Particles, Oladayo Ayobami Oyebanji
Browse all Theses and Dissertations
Photodynamic therapy (PDT) involves the use of light at an appropriate wavelength acting on a photosensitizing chemical to cause cell death via generation of reactive oxygen species. PDT has been useful in the management of skin conditions (like acne, psoriasis) and cancers like superficial skin, esophageal and non-small cell lung cancers. In addition to these therapeutic effects, previous murine studies from our group have demonstrated that topical PDT induces immunosuppression in vivo. Thus, topical PDT of skin can generate systemic effects through unknown mechanisms. Our group showed that PDT induces an immunosuppressive effect which occurs partly via Platelet-Activating Factor Receptor …
Role Of Exercise In Macrophage Polarization Of Perivascular Adipose Tissue And Adipose Tissue Inflammation In Hypertensive Mice Model, Venkata Sai Usha Sri Polaki
Role Of Exercise In Macrophage Polarization Of Perivascular Adipose Tissue And Adipose Tissue Inflammation In Hypertensive Mice Model, Venkata Sai Usha Sri Polaki
Browse all Theses and Dissertations
Hypertension, also known as high blood pressure, is a long-term medical condition in which the blood pressure in the arteries is persistently elevated. It can lead to severe health complications and increase the risk of heart disease, stroke, and sometimes death. Perivascular adipose tissue (PVAT) is known as the adipose tissue (AT) surrounding all the blood vessels and plays a critical role in the pathogenesis of the vascular disease. In vascular pathologies, PVAT increases in volume and becomes dysfunctional, with altered cellular composition and molecular characteristics. Macrophages have been found to accumulate in PVAT during hypertension, which might be involved …
Effects Of Creatine And Nicotinamide On Experimentally Induced Senescence In Dermal Fibroblasts, Avinash Satyanarayan Mahajan
Effects Of Creatine And Nicotinamide On Experimentally Induced Senescence In Dermal Fibroblasts, Avinash Satyanarayan Mahajan
Browse all Theses and Dissertations
Dermal fibroblasts provide structural support by producing collagen and other structural/support proteins beneath the epidermis. Fibroblasts also produce Insulin-like growth factor-1 (IGF-1), which binds to the IGF-1 receptors (IGF-1Rs) on keratinocytes to activate signaling pathways that regulate cell proliferation and cellular responses to genotoxic stressors like ultraviolet B radiation found in sunlight. Our group has determined that the lack of IGF-1 expression due to fibroblast senescence in the dermis of geriatric individuals is correlated with an increased incidence of skin cancer in geriatric patients. The present studies were designed to test the hypothesis that pro-energetics like creatine monohydrate and nicotinamide …
The Feeding Pattern Of C57bl/6j Mice, Yakshkumar Dilipbhai Rathod
The Feeding Pattern Of C57bl/6j Mice, Yakshkumar Dilipbhai Rathod
Browse all Theses and Dissertations
The feeding pattern and control of energy intake in social mice are poorly understood. Here, we determined and quantify the normal nocturnal feeding pattern of group-housed male and female mice of the C57BL/6J genetic background fed a chow diet. Mice at 10 and 20weeks of age showed the expected age-dependent increase in lean and fat mass being them more pronounced in males. Under ad libitum conditions, male and female mice ate comparable amounts of food per unit of body weight irrespective of gender. The analysis of the feeding microstructure revealed that energy intake in females is the result of significantly …
Differing Functions Of Atr Kinase In Human Epidermal Keratinocytes Exposed To Ultraviolet B Radiation, Kavya Shaj
Differing Functions Of Atr Kinase In Human Epidermal Keratinocytes Exposed To Ultraviolet B Radiation, Kavya Shaj
Browse all Theses and Dissertations
Ataxia-telangiectasia and Rad-3 related (ATR) is a DNA damage response protein kinase that is upregulated in various cancer cell types and has key roles in cell cycle progression, DNA repair and apoptosis. Small molecule ATR kinase inhibitors are of importance as antineoplastic agents because they sensitize cancer cells to DNA damaging chemotherapy drugs. However, only a few studies have looked at the effects of ATR kinase inhibitors on non-replicating cells, which constitute the majority of cells in the human body and have suggested an opposite, pro-apoptotic role for ATR kinase signaling in non-replicating cells in comparison to a pro-survival function …
Kinetics Of Microvesicle Particle Release In Keratinocytes, Pariksha Thapa
Kinetics Of Microvesicle Particle Release In Keratinocytes, Pariksha Thapa
Browse all Theses and Dissertations
Microvesicle particles (MVPs) are subcellular particles that could be involved in inter-cellular communication because they carry various bioactive substances including cytokines. Previous studies from our lab has shown that that the lipid mediator Platelet-activating Factor (1-alkyl-2-acetyl-glycerophosphocholine; PAF) and ultraviolet B radiation (UVB) enhances the release of MVP in various cell types like primary keratinocytes, epithelial cell lines, and murine skin. We hypothesized that there may be synergistic increases in MVP release after combination of treatment of keratinocytes with UVB and PAF agonist (CPAF)/phorbol ester (PMA). The combination treatment significantly increases MVP and cytokine release at 4 to 8 hours time …
Effect Of Endothelial Progenitor Cell-Derived Exosomes On High Glucose And Hypoxia/ Reoxygenation-Induced Injury Of Astrocytes, Manasi Suchit Halurkar
Effect Of Endothelial Progenitor Cell-Derived Exosomes On High Glucose And Hypoxia/ Reoxygenation-Induced Injury Of Astrocytes, Manasi Suchit Halurkar
Browse all Theses and Dissertations
In this study, we tested the protective effects of EPC-EXs (endothelial progenitor cell derived exosomes) and miR-126 EPC-EXs (microRNA-126 EPC-EXs) on the astrocytes injured by HG (high glucose) plus H/R (hypoxia/reoxygenation) model. At first, we determined the concentration and time dependent uptake of EPC-EXs by astrocytes. It was also found that the EPC-EXs were uptaken via macropinocytosis, caveolin-dependent and clathrin-mediated pathways in astrocytes. Furthermore, the astrocyte cell line was injured through the HG + H/R model. EXs, isolated by ultracentrifugation from the EPC culture supernatant were co-incubated with the injured cells. It was found that EPC-EXs and miR-126 EPC-EXs decrease …