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Investigating The Suitability Of The Crayfish Metabolome As A Bioindicator Of Stream Conditions, Natalie M. Izral
Investigating The Suitability Of The Crayfish Metabolome As A Bioindicator Of Stream Conditions, Natalie M. Izral
Electronic Thesis and Dissertation Repository
The suitability of the crayfish metabolome as a bioindicator of stream conditions was investigated because there is a need for fast responding, sensitive, diagnostic, and ecologically relevant bioindicators. A laboratory study assessed Procambarus clarkii metabolome sensitivity to six different treatments of food or DO stress. A field study in subcatchments of the Red River Valley, Manitoba, Canada assessed the effects of land-use type (reference, agriculture, wastewater effluent) on the Orconectes virilis metabolome. Crayfish were sacrificed, and hepatopancreas, gill, and tail tissues were dissected. Analyses found the metabolome was sensitive enough to potentially diagnose food and DO stress in P. clarkii …
Response Of Soil Nitrification To The Veterinary Pharmaceuticals Monensin, Ivermectin And Zinc Bacitracin, Magda A. Konopka
Response Of Soil Nitrification To The Veterinary Pharmaceuticals Monensin, Ivermectin And Zinc Bacitracin, Magda A. Konopka
Electronic Thesis and Dissertation Repository
Pharmaceutical residues can reach agricultural land through amendment with animal or human waste. Since 2010, a series of replicated plots received annual applications of ivermectin, monensin and zinc bacitracin, either singly or in a mixture, at 0.1 mg/kg and 10 mg/kg concentrations. I collected soil samples before and after the fourth annual application of pharmaceuticals and assayed them for functional changes and amoA gene abundance, a gene needed for ammonia oxidation. In 2013, I exposed the soils to 100 mg/kg in a laboratory experiment which resulted in acceleration of nitrification. Under 10 mg/kg treatments in the field the abundance of …