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Full-Text Articles in Life Sciences
Accounting For Molecular Mobility In Structure Determination Based On Nuclear Magnetic Resonance Spectroscopic And X-Ray Diffraction Data, Wilfred Van Gunsteren, Roger Brunne, P. Gros, René Van Schaik, Celia Schiffer, Andrew Torda
Accounting For Molecular Mobility In Structure Determination Based On Nuclear Magnetic Resonance Spectroscopic And X-Ray Diffraction Data, Wilfred Van Gunsteren, Roger Brunne, P. Gros, René Van Schaik, Celia Schiffer, Andrew Torda
Celia A. Schiffer
No abstract provided.
Simultaneous Refinement Of The Structure Of Bpti Against Nmr Data Measured In Solution And X-Ray Diffraction Data Measured In Single Crystals, Celia Schiffer, Robert Huber, Kurt Wuthrich, Wilfred Van Gunsteren
Simultaneous Refinement Of The Structure Of Bpti Against Nmr Data Measured In Solution And X-Ray Diffraction Data Measured In Single Crystals, Celia Schiffer, Robert Huber, Kurt Wuthrich, Wilfred Van Gunsteren
Celia A. Schiffer
The structure of the bovine pancreatic trypsin inhibitor (BPTI) has been determined to high resolution by both NMR spectroscopy in solution and X-ray diffraction in crystals. The root-mean-square difference calculated between the two structures for the polypeptide backbone is 0.9 A. Several amino acid side-chains, of which all but one are charged or polar, have different conformations. We find that by refining one structure simultaneously against both the NMR and crystallographic data sets, it can accommodate both. Different starting configurations were used, including the X-ray structure 5pti, an NMR conformer, and the X-ray structure in the full unit cell with …
Structural Stability Of Disulfide Mutants Of Basic Pancreatic Trypsin Inhibitor: A Molecular Dynamics Study, Celia Schiffer, Wilfred Van Gunsteren
Structural Stability Of Disulfide Mutants Of Basic Pancreatic Trypsin Inhibitor: A Molecular Dynamics Study, Celia Schiffer, Wilfred Van Gunsteren
Celia A. Schiffer
The structure and folding of basic pancreatic trypsin inhibitor (BPTI) has been studied extensively by experimental means. We report a computer simulation study of the structural stability of various disulfide mutants of BPTI, involving eight 250-psec molecular dynamics simulations of the proteins in water, with and without a phosphate counterion. The presence of the latter alters the relative stability of the single disulfide species [5-55] and [30-51]. This conclusion can explain results of mutational studies and the conservation of residues in homologues of BPTI, and suggests a possible role of ions in stabilizing one intermediate over another in unfolding or …
Accessibility And Order Of Water Sites In And Around Proteins: A Crystallographic Time-Averaging Study, Celia Schiffer, Wilfred Van Gunsteren
Accessibility And Order Of Water Sites In And Around Proteins: A Crystallographic Time-Averaging Study, Celia Schiffer, Wilfred Van Gunsteren
Celia A. Schiffer
Water plays an essential role in most biological processes. Water molecules solvating biomolecules are generally in fast exchange with the environment. Nevertheless, well-defined electron density is seen for water associated with proteins whose crystal structure is determined to high resolution. The relative accessibility of these water sites is likely to be relevant to their biological role but is difficult to assess. A time-averaging crystallographic refinement simulation on basic pancreatic trypsin inhibitor successfully characterizes the relative accessibility of the crystallographic water sites. In such a refinement simulation water diffuses through the crystal lattice in a manner that is consistent with the …