Life Sciences Commons^™

In Situ Regulation Of Cytosolic Phospolipase A₂, Beverly A. Rzigalinski

Theses and Dissertations in Biomedical Sciences

The 85 kDa cytosolic phospholipase A₂ (cPLA₂) is an agonist-responsive effector for intracellular signal transduction through the arachidonate cascade. In vitro studies have demonstrated that this enzyme is regulated by sub-micromolar calcium and is specific for arachidonate as the sn-2 fatty acyl group of phospholipid substrates. However, very little data is available regarding in situ mechanisms which govern the activity of cPLA₂. The primarily objective of these studies was to develop an in situ system for the study of cPLA₂, and investigate mobilization of arachidonate during signal transduction events.

Dimethylsulfoxide differentiation of the …

Cerebral Microcirculatory Effects Of Maturation, Scott R. Elliott

Loma Linda University Electronic Theses, Dissertations & Projects

The present studies demonstrate that in the second (2B) and fourth (4B) branches of newborn and adult sheep middle cerebral arteries, 5-HT2 serotonergic receptors mediate contractile responses to serotonin and that alpha-1 adrenergic receptors mediate contractile responses to norepinephrine. In addition, tissue sensitivity to 5-HT and NE decreases significantly with maturation, but does not vary with branch order. These age-related changes were associated with a decrease in affinity and maximum response in 2B and 4B segments of 5-HT and NE contracted arteries, respectively. Norepinephrine and 5-HT occupancy at the pD₂ rose significantly in 2B segments, but did not change …

Antithrombotic Activity Of Clove Oil, Shaikh A. Saeed, A H. Gilani

Department of Biological & Biomedical Sciences

Clove oil inhibited human platelet aggregation induced by arachidonic acid (AA), platelet-activating factor (PAF) or collagen. Clove oil was a more effective inhibitor for aggregation induced by AA and PAF (IC50:4 and 6 microM respectively) than collagen (IC50: 132 microM). The in vivo experiments in rabbits showed that clove oil (50-100 mg/kg) afforded 100% protection against PAF (11 mg/kg, i.v.) and 70% protection against AA (2.0 mg/kg, i.v.)-induced thrombosis and shock due to pulmonary platelet thrombosis. It also inhibited thromboxane-A2 and 12-HETE production by human platelets incubated with [C14] AA. These results are indicative that clove oil is inhibitory of …

Differential Role Of The Endothelium In Regulating Microvascular Blood Flow, Tao Tang

Electronic Theses and Dissertations

The vascular endothelial cell (EC) plays an important role in regulating vascular tone and local blood flow by sensing chemical and mechanical stimuli on the vascular wall and releasing a host of vasoactive substances upon activations of endogenous or exogenous vasoactive substances. The central hypothesis is that local control of blood now and autoregulatory behavior in the microcirculation is distinctive at different levels of the vasculature and is dependent on the cellular activities of the EC and its interaction with the local environment. The in vivo as well as the ex vivo, flow-controlled preparations of the hamster cheek pouch were …

Characterization Of The Vasoactivity Of Tachykinins In Isolated Rat Kidney: Functional Studies And In Vitro Receptor Autoradiography, Yuejin Chen

Electronic Theses and Dissertations

Although tachykinins have potent vascular actions, their effect on renal resistance blood vessels is currently unknown. The vasoactive properties of tachykinins and related analogs were assessed in isolated perfused rat kidney. At a basal perfusion pressure (PP) of 75 $\pm$ 6 mm Hg (n = 5), bolus injections of substance P (SP) had no significant vasoactive effect. Following a sustained increase in baseline PP (134 $\pm$ 10 mm Hg) produced by phenylephrine (1 $\mu$M), SP evoked a dose-dependent increase in PP. The largest dose of SP increased PP by 60 $\pm$ 5 mm Hg. The vasoconstrictor response to SP was …

Denervation Supersensitivity Of The Rat Vas Deferens: A Role For Protein Kinase C, Sonny T. Abraham

Electronic Theses and Dissertations

A role for protein kinase C (PKC) in the denervation-induced supersensitivity of the rat vas deferens was investigated. Chronic, surgical denervation of the rat vas deferens (up to 8 days) resulted in tissues that produced enhanced contractile responses to norepinephrine (NE) in isolated organ baths. Single challenges of NE (10 $\mu$M) produced 0.6 $\pm$ 0.1 g of maximal tension in the control vas whereas in the paired, denervated tissue 2.2 $\pm$ 0.3 g of tension was recorded (n = 6). Cumulative concentration-effect curves to NE produced in the denervated vas deferens were shifted 18-fold to the left of the control …

Possible Presence Of Calcium Channel Blocker(S) In Rubia Cordifolia: An Indigenous Medicinal Plant, A H. Gilani, K H. Janbaz, M Zaman, A Lateef, A Suria, H R. Ahmed

Department of Biological & Biomedical Sciences

Crude extract of Rubia cordifolia (RC) was tested in isolated tissue preparations for its possible calcium channel antagonistic activity. RC suppressed the spontaneous contractions of guinea-pig atria, rabbit jejunum and rat uterus in a concentration dependent manner (0.1-3 mg/ml). In rabbit aorta, it inhibited norepinephrine (10 microM) and KCl (80 mM) induced contractions. Replacement of physiological salt solution with calcium free solution abolished the spontaneous movements of rabbit jejunum. However, addition of calcium (25 micrograms/ml) in the tissue bath restored the spontaneous movements. When the tissues were pretreated with plant extract (1 mg/ml) or verapamil (0.5 microgram/ml), addition of calcium …

Hepatoprotective Effects Of Artemisia Scoparia Against Carbon Tetrachloride: An Environmental Contaminant, A H. Gilani, K H. Janbaz

Department of Biological & Biomedical Sciences

The hepatoprotective activity of crude extract of artemisia scoparia (aerial parts) was investigated against experimentally produced hepatic damage using carbon tetrachloride (CCl4) as a model hepatotoxin. CCl4 at the dose of 1.5 ml/kg, produced liver damage in rats as manifested by the rise in serum levels of AST and ALT to 395 +/- 110 and 258 +/- 61 IU/l (mean +/- SEM; n = 10) respectively, compared to control values of 106 +/- 15 and 26 +/- 04. Pretreatment of rats with plant extract (150 mg/kg) significantly lowered (P < 0.01), the respective serum GOT and GPT levels to 93 +/- 05 and 27 +/- 03 IU/l, indicating hepatoprotective action. Pentobarbital sodium (75 mg/kg)-induced sleeping time in mice was found to be 140.8 +/- 1.5 min (n = 10) which was similar (P > 0.05) to that obtained in the group of animals pretreated with …

The Interaction Of O6-Benzylguanine And Streptozotocin In The Inactivation Of O6-Methylguanine Dna Methyltransferase And The Reversal Of 1,3 Bis (2-Chloroethyl)-1-Nitrosourea Resistance, Upendra Kumar Marathi

Master's Theses

No abstract provided.

Subject Index, Author Index(ดัชนีคำสำคัญ, ดัชนีผู้แต่ง), N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Dopaminergic Modulation Of Basal Forebrain Cholinergic Neurons, Mary Elizabeth Muench

Master's Theses

No abstract provided.

Optimization Of Ion-Pair High Performance Liquid Chromatography For Separation Of Endogenous Amines And Their Metabolites In Rat Brain Tissue(การหาภาวะเหมาะที่สุดสำหรับการวิเคราะห..., Rungrudee Srisawat, Anan Srikiatkhachorn, Naiphinich Kotchabhakdi, Duangdeun Meksuriyen

The Thai Journal of Pharmaceutical Sciences

An improved, HPLC with electrochemical detection method for the isocratic separation and simultaneous determination of endogenous amines and their metabolites from post-mortem rat brain tissue is described. Optimal conditions that maximize stability, res...

Role Of The Pharmacist In Support Of The Who Revised Drug Strategy(บทบาทของเภสัชกรในการสนับสนุนแผนงานด้านยาขององค์การอนามัยโลก), นิยดา เกียรติยิ่งอังศุลี

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Jsps-Nrct Core University Exchange System On Pharmaceutical Sciences, Second Joint Seminar, Recent Advances On Pharmaceutics And Pharmaceutical Technology, N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

บรรณาธิการแถลง, N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

บรรณาธิการ : การสั่งงานด้วยแผนภูมิ, ประศาสน์ เจริญพานิช

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

ยาฉีดเภสัชภัณฑ์รังสี Tc-99m Human Serum Albumin, สง ยุตยาจาร, ศึริศักดี้ ดำรงพิศุทธิกุล, พ.ท.หญิงสุเนตรา ดำรงพิศุทธิกุล

The Thai Journal of Pharmaceutical Sciences

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Effects Of Manufacturing Methods On Physical Properties Of Isoniazid And Diazepam Tablets(อิทธิพลของกรรมวิธีการผลิตต่อคุณสมบัติทางกายภาพของ ยาเม็..., Sirisak Dumrongpisuthigul, Song Yootyachan

The Thai Journal of Pharmaceutical Sciences

The effects of four manufacturing methods; Direct compression, Dry granulation, Wet granulation and Microgranulation were evaluated on 7 formula of Isoniazid and Diazepam tablets. The physical properties: Weight variation Hardness, Friability, Disintegra...

Discovery And Utilization Of Dermatological Products From Medicinal Plants In Thailand Part Ii: An Evidence For Utilization Of The Essential Oil From Anisomoles Indica, Siriporn Okonogi, Yanee Pongpaibul, Isamu Murakoshi

The Thai Journal of Pharmaceutical Sciences

The essential oil from Anisomeles indica (Labiatae) was incorporated into different types of dermatological bases. The release of essential oil from the bases showed that only some formulations were capable of releasing the active ingredient to inhibit t...