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Optimal Culture Conditions For The Propagation Of The Oyster Pathogen Perkinsus Marinus (Apicomplexa) In Protein Deficient Medium, Jf Lapeyre, M Faisal
Optimal Culture Conditions For The Propagation Of The Oyster Pathogen Perkinsus Marinus (Apicomplexa) In Protein Deficient Medium, Jf Lapeyre, M Faisal
VIMS Articles
The protozoan, Perkinsus marinus, acclimated and proliferated in the culture medium JL-ODRP-1 without bovine serum albumin. The principal culture conditions for the optimal propagation of P. marinus in this protein deficient culture medium, were determined. The greatest growth rates of the parasite occurred at 28 degrees C, 661 mOsm/kg and pH 7.5. Decreasing seeding densities from 16 x 10(5) to 1 x 10(5) also increased growth rate. After several passages in the absence of 5% CO2 tension, the growth rate of P. marinus was similar to its original value in the presence of 5% CO2 tension. This protein deficient culture …