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Full-Text Articles in Life Sciences
Infection And Transovarial Transmission Of Rickettsiae In Dermacentor Variabilis Acquired By Artificial Feeding, K. R. Macaluso, Daniel E. Sonenshine, Shane M. Ceraul, A. F. Azad
Infection And Transovarial Transmission Of Rickettsiae In Dermacentor Variabilis Acquired By Artificial Feeding, K. R. Macaluso, Daniel E. Sonenshine, Shane M. Ceraul, A. F. Azad
Biological Sciences Faculty Publications
In this study we examined the efficiency of an in vitro feeding technique using glass microcapillaries as a method of establishing rickettsiae-infected lines of ticks. To quantify the volume ingested by ticks during microcapillary feeding, the incorporation of radiolabeled amino acids in tick gut and hemolymph was calculated. Fifteen of 18 ticks consumed between 0.06 μl and 6.77μl. However, ingestion of fluid was not correlated to weight gain during capillary feeding. Uninfected and partially fed laboratory-reared female Dermacentor variabilis ticks were exposed to either Rickettsia montana- or Rickettsia rhipicephali-infected Vero cells via microcapillary tubes, returned to rabbit hosts, …
Tick Immunity To Microbial Infections: Control Of Representative Bacteria In The Hard Tick Dermacentor Variabilis (Acari: Ixodidae), Robert Johns, Shane Ceraul, Daniel E. Sonenshine, Wayne L. Hynes
Tick Immunity To Microbial Infections: Control Of Representative Bacteria In The Hard Tick Dermacentor Variabilis (Acari: Ixodidae), Robert Johns, Shane Ceraul, Daniel E. Sonenshine, Wayne L. Hynes
Biological Sciences Faculty Publications
No abstract provided.
Contrasts In Tick Innate Immune Responses To Borrelia Burgdorferi Challenge: Immuno-Tolerance In Ixodes Scapularis Versus Immunocompetence In Dermacentor Variabilis (Acari: Ixodidae)., Robert Johns, Jun Ohnishi, Anne Broadwater, Daniel E. Sonenshine, Aravinda M. Desilva, Wayne L. Hynes
Contrasts In Tick Innate Immune Responses To Borrelia Burgdorferi Challenge: Immuno-Tolerance In Ixodes Scapularis Versus Immunocompetence In Dermacentor Variabilis (Acari: Ixodidae)., Robert Johns, Jun Ohnishi, Anne Broadwater, Daniel E. Sonenshine, Aravinda M. Desilva, Wayne L. Hynes
Biological Sciences Faculty Publications
The blacklegged tick, Ixodes scapularis Say, transmits the Lyme disease spirochete (Borrelia burgdorferi), whereas the American dog tick, Dermacentor variabilis (Say), is unable to transmit the bacterium. We compared the innate immune response of these ticks against spirochetes directly inoculated into the hemocoel cavity of ticks. In I. scapularis, some Borrelia were found associated with hemocytes, while numerous other spiral-shaped, intact bacteria remained free in the hemolymph. In contrast, in D. variabilis only remnants of the bacteria were evident in the hemolymph, indicating lysis; intact spirochetes were rare. Spirochetes were observed bound to or within the organs …
Identification Of A Defensin From The Hemolymph Of The American Dog Tick, Dermacentor Variabilis, Robert Johns, Daniel E. Sonenshine, Wayne L. Hynes
Identification Of A Defensin From The Hemolymph Of The American Dog Tick, Dermacentor Variabilis, Robert Johns, Daniel E. Sonenshine, Wayne L. Hynes
Biological Sciences Faculty Publications
Hemolymph from partially fed virgin Dermacentor variabilis females was collected following Borrelia burgdorferi challenge and assayed for antimicrobial activity against Bacillus subtilis and B. burgdorferi. A small inducible cationic peptide was identified by SDS-PAGE in the hemolymph of these ticks as early as 1h post challenge. Following purification by a three-step procedure involving sequential SepPak elution, reversed phase high performance liquid chromatography (RP-HPLC) and gel electrophoresis, the yield of the active peptide was approximately 0.1% of the total protein in the hemolymph plasma. The molecular weight, 4.2kDa, was determined by MALDI-TOF mass spectrometry. N-terminal sequencing by the Edman degradation …