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Full-Text Articles in Life Sciences
The Art Of Amphibian Conservation: Linking In-Situ And Ex-Situ Populations Of Endangered Species Through Genome Banking, Isabella Joann Burger
The Art Of Amphibian Conservation: Linking In-Situ And Ex-Situ Populations Of Endangered Species Through Genome Banking, Isabella Joann Burger
Theses and Dissertations
Limited breeding success in captive breeding programs has necessitated the development of assisted reproductive technologies (ART) to preserve and increase genetic variation and population numbers of both captive and wild amphibian groups. ART has been shown to be successful in numerous anuran species, and current studies focus on the application of ART in ex-situ populations. The focus of this project is to show that linking in-situ and ex-situ amphibian populations through sperm cryopreservation, genome banking, and in-vitro fertilization is possible, with the goal of increasing gene diversity throughout groups in order to produce self-sustaining, wild populations in the future. Specific …
Lipid Quantification And Cryopreservation Of In Vitro Produced Jersey Cattle Embryos, Katherine A. Rhodes-Long
Lipid Quantification And Cryopreservation Of In Vitro Produced Jersey Cattle Embryos, Katherine A. Rhodes-Long
Master's Theses
Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have darker cytoplasm than their in vivo-derived counterparts because of higher lipid accumulation. High lipid accumulation is associated with impaired embryo quality. Forskolin is an adenylate cyclase activator that regulates cAMP levels in cells and has been shown to induce lipolysis in IVP embryos. L-carnitine is required for transport of fatty acids from the intermembrane space of …
Open Pulled Straw (Ops) Vitrification Of Mus Musculus Morula And Blastocyst Survival In Two Common Cryopreservation Medias, Gina Marie Cirimele
Open Pulled Straw (Ops) Vitrification Of Mus Musculus Morula And Blastocyst Survival In Two Common Cryopreservation Medias, Gina Marie Cirimele
Animal Science
The objectives of this study were to: (1) determine an optimal cryoprotectant of mice embryos; and (2) determine whether morula or blastocyst stage is ideal for vitrification in both medias. One experiment was performed using two different medias for vitrification with open pulled straws (OPS) with morulae and blastocysts. In the first protocol, we called V, embryos were exposed to 10% ethylene glycol (EG) for 5 minutes, then 40% ethylene glycol and 0.6 M galactose for about 30 seconds, loaded into an OPS, and plunged into liquid nitrogen. In the second protocol, we called VG, embryos were exposed to 7.5% …