Life Sciences Commons^™

Characterization Of The Swine Gut Microbiome Dynamics, Culturomics And Probiotics, Xiaofan Wang

Graduate Theses and Dissertations

The gastrointestinal tract is a critical place where the immune system concentrates many of its protective functions. It exerts both physical and biochemical defenses against invading foodborne pathogens and other hazards. In pigs, the gut microbiota community assembled postnatal plays a very important role in the development of multiple physiologic aspects, such as development of the gastrointestinal tract, nutrient exchange, immune defense against foreign pathogens and growth performance. Microbiome-host communication changes with age. Despite recent advances in understanding the swine gut microbiome at different growth stages, a comprehensive longitudinal study of the lifelong dynamics of the swine gut microbiome is …

The Effects Of Super-Dosing Phytase On Nursery And Grower Pigs, Joshua Paul Knapp

Graduate Theses and Dissertations

To determine the effects of super-dosing on pigs fed phosphorous and calcium adequate or deficient diets (Experiment 1); the optimum level of corn express phytase (CEP, Experiment 2); and the effects of xylanase/phytase on growth performance (Experiment 3) in pigs fed reduce nutrient diets, weaned pigs were blocked with initial body weight (BW) and allotted to dietary treatments. Treatments were: Positive control (PC), P/Ca adequate diets (NRC 2012); Negative Control (NC), low P (-0.15%) and Ca (-0.12%), +0, 1000, and 4000 FTU/kg of CEP in both PC and NC (Experiment 1); PC, P/Ca adequate diets; NC, decreased P (0.15%) and …

Broiler Breeder Mating Behavior And The Effects Of Pre-Incubation Embryo Integrity Modifications On Broiler Performance, Emily Kathryn Lhamon

Graduate Theses and Dissertations

Modern broiler breeders have been observed displaying significantly less mating behaviors than their ancestral fowl. Effective mating behavior translates into fertile hatching eggs that ultimately become broilers processed for market. The poultry industry has made significant changes in genetics, environment, housing, and diets of commercial poultry. However, there are some concessions made in genetic selection and streamlined management to produce larger, fast-growing, efficient birds. Aggression in broiler breeder males is believed to be a sign of high fertility. However, highly aggressive males pose a threat to the welfare of their flock mates, and may also impede frequency of mating and …

The Effect Of Alternative Fertilization And Application Time On Voluntary Intake Of Forages, Jessica Clark

Graduate Theses and Dissertations

Dairy slurry and poultry litter have increased in popularity as fertilizers in the agriculture industry. However, residual effects of these manures on voluntary intake of forages from subsequent harvests are not well known. The objectives of this two part study were to determine if moisture level of alfalfa silage and the use of dairy slurry as a fertilizer have an effect on intake and digestibility by sheep, and if forage species and the use of poultry litter as a fertilizer have an effect on intake by horses. Eighteen ewes (47.6 ± 5.34 kg) were used in experiment 1 and were …

A Cradle To Farm Gate Life Cycle Analysis Of Land Use In U.S. Pork Production, William Benjamin Putman

Graduate Theses and Dissertations

The goal of this study was to conduct a detailed Life Cycle Assessment (LCA) of the U.S. live swine production supply chain to quantify land use requirements and to assess the impact associated with various ration compositions. The functional unit was defined as one kilogram (2.2 pounds) of live swine at the farm gate, ready for transport to the abattoir. This assessment focused on the three highest producing USDA regions, which encompassed the Midwest (Regions 5 and 7) and the Southeast (Region 4), representing 86% of U.S. market hog production.

First, a literature review was conducted to summarize the most …

Life Cycle Assessment Of Alternative Swine Management Practices, Prathamesh Avadhut Bandekar

Graduate Theses and Dissertations

Life Cycle Assessments (LCAs) are quantitative analyses of complex systems for evaluation of impacts and risk associated with management decisions. LCAs can be effective tools for determining comparative advantages of management strategies across specific impact concern. In this study, life cycle assessments of pork production management alternatives was performed. The alternative management practices included in this study were production of entire males (boars), use of pens for gestation housing, immunocastration, production without growth promoting antimicrobials, production without growth promoting and preventive antimicrobials, and production without ractopamine. These LCAs evaluated the impact of each management strategy on greenhouse gas emission (GHG), …

Effect Of Feeding Regimen And Age On Lipid Metabolism In Broiler Breeder Hens And Progeny, Nirun Boonsinchai

Graduate Theses and Dissertations

Age of hens and feed allocation can affect egg yolk lipid compositions which in turn directly relate to chick quality. A series of experiments were conducted to determine the effects of age and feeding programs on lipid metabolism in breeder hens and progeny. In the first trial, the rate of yolk absorption and fatty acid profiles in the yolk were compared among 28, 35, and 49wk old hens. The results revealed that the rate of yolk absorption was higher, but the absolute amount of fatty acids absorbed was lower in young hens than in the old hens. The second trial …

Effects Of Crystalline Amino Acid Supplementation Of Reduced Crude Protein (Rcp) Diet On Net Energy Basis On Growing-Finishing Swine, Daniel Cook

Graduate Theses and Dissertations

Barrows and gilts (n=210) were used to test the effects of crystalline amino acid (AA) supplementation of reduced crude protein (RCP) diets formulated on a net energy basis on quality characteristics of the LM from growing-finishing pigs. Pigs were blocked by weight, and pens (3 barrows and 3 gilts/pen) within each block were assigned randomly to either corn-soybean meal diets (C) or 1 of 3 RCP diets and added crystalline AA levels for the dietary treatments during each feeding phase. During the last 3-wk feeding phase, 10 ppm of ractopamine were included in all diets. At slaughter and after a …

Effect Of Supplemental Trace Mineral (Zinc, Copper, And Manganese) Source On Growth Performance, Morbidity, And Trace Mineral Status In Beef Cattle, Anthony Ryan

Graduate Theses and Dissertations

A series of studies were conducted to determine the effect of supplemental trace mineral source on growth performance, morbidity, and trace mineral status in growing beef cattle. The first experiment evaluated supplemental trace minerals from sulfate, organic, or hydroxy sources on growth performance and morbidity. Crossbreed beef calves were assigned to 1 of 3 treatments consisting of supplemental zinc (360 mg/d), copper (125 mg/d), and manganese (200 mg/d) from inorganic, organic, or hydroxy sources fed daily over a 42 to 45-d backgrounding phase. After removal of chronic and dead calves from the data set, trace mineral source had no effect …

Influence Of Steroids And Gonadotropins On Reproduction In Beef Cattle, Thomas L. Devine

Graduate Theses and Dissertations

The objective of this thesis was to evaluate the influence of steroids and gonadotropins on reproduction in beef cattle. In experiment 1, beef heifers were used to determine the influence of growth-promoting implants on growth, reproductive development, estrous behavior, and pregnancy rate. Heifers were assigned to 1 of 4 implant treatment groups: control (CON); trenbolone acetate (TBA); trenbolone acetate plus estradiol (TBA+E2) or zeranol (ZER). Heifers were implanted once, A.I. and exposed to bull during this experiment. Body weight, BCS, HH, RTS, estrous behavior and pregnancy data were collected throughout this experiment. Average daily gain of heifers was greater for …

Assessing The Effectiveness Of Student Oriented Learning Outlines (Solos) In An Equine Classroom, Kathleen S. Jogan

Graduate Theses and Dissertations

This study determined if the use of the student oriented learning outline (SOLO) in a University of Arkansas equine production classroom had a positive influence in three areas: mastery of material taught, retention of material taught and voluntary positive student behaviors related to the use of course material. Thirty-one students who were enrolled in 2012 were in the non-SOLO group (control), and 25 students who were enrolled in 2013 were the SOLO group (treatment). Three separate units were taught to the treatment and control groups with only one difference: the treatment group was given a SOLO one week in advance …

Balking Incidence And Behavioral Responses During Handling In Fed Cattle With Genetic And Carcass Implications, Michelle Lynn Thomas

Graduate Theses and Dissertations

Temperament in cattle is often defined as the reactivity to human handling or novel environments. Temperament differences have been shown among breed-type categories, within breed types, among crossbreds, and between sexes. Temperament tests are typically completed at weaning time on beef cattle, and rarely on fed Holstein steers for beef production. Balking behavior, or cease in forward motion, in the cattle working facility can pose welfare issues as the electric prod use to coerce movement is implemented. Three observational field projects were designed to evaluate balking behavior incidence in unknown breed-types at the processing plant, Holstein steers in the feedlot …

The Effect Of Calcium And Nonphytate Phosphorus On Chicken Genetic Line, Egg Production, Shell Quality, Bone Homeostasis, And Progeny Bone Quality, Phiphob Sodsee

Graduate Theses and Dissertations

The study consisted of two parts to determine effects of dietary calcium (Ca) and nonphytate phosphorus (NPP) level on four pure genetic lines of broiler breeders. The first part was concentrated on body composition, egg production and shell quality changes according to diet and genetic line. The second part was the study of bone homeostasis variables combining hematology, bone histology and body composition changes according to diet and genetic line. In the study, pullets (246 from each of pure genetic line A, B, C and D) were individually caged and light stimulated at 21 weeks of age. The study duration …

Evaluating Economic And Environmental Impacts Of Changing Herd Sire Genetics On Arkansas Cow-Calf Operations, Daniel Keeton

Graduate Theses and Dissertations

This research looks at impacts of changing sires, and thus bull genetics, on cow-calf operations of varying land and herd sizes in the state of Arkansas. A 2012 Across-Breed EPD table, adjusted to the Angus breed, was used to estimate breed sire effects on birth weight and weaning weight of the herd's offspring (Kuehn and Thallman 2012a). Together with modified animal performance, the use of 2012 and most recent five- and ten- year Arkansas state average prices, allowed the evaluation of changing herd sires by accounting for breed type and hide color differences in prices. Using a spreadsheet tool that …

Effects Of Ergot Alkaloids And Bovine Bodily Fluids On Cytochrome P450 Activity, Nicholas Scott Ezell

Graduate Theses and Dissertations

This thesis evaluates the Promega^TM P450-Glo assay (Promega^TM V9800) as a tool for quantifying ergot alkaloid concentration. Current techniques used for detection of ergot alkaloids are slow and expensive, do not detect all ergot alkaloids, or are not effective on bovine bodily fluids. The first study was conducted to determine effects of commercial ergot alkaloids (n = 6; 0 - 400 μM) on the Promega^TM P450-Glo assay. Cytochrome P450 (CYP450) activity in assay had a differential response to each ergot alkaloid and concentration. As concentrations of ergotamine, dihydroergotamine, ergocornine, and ergocryptine increased CYP450 activity was inhibited (P …

Relationship Of Lactate Dehydrogenase & Prolactin Genotypes To Immune Function, Growth, And Composition Of Replacement Heifers Grazing Tall Fescue, Christina Marie Turner

Graduate Theses and Dissertations

Objectives of this research were to determine effects of LDH enzymatic activity and circulating PRL on immune response, growth, composition, and reproduction, and to determine the effects of single nucleotide polymorphisms of LDH and PRL on immune function, growth and carcass traits, cyclicty, and fecundity of replacement heifers grazing endophyte infected tall fescue. Eighty-nine Angus based heifers had physical and ultrasound measurements along with blood samples collected at weaning, yearling, and prebreeding. Estrus detection was conducted for 10 d post each collection period, except prebreeding. Pregnancy status was verified after AI and again after bull removal. Heifers were maintained on …

Timing Of Castration, With Or Without Analgesia, On Growth Performance, Hematology, And Behavior Of Beef Calves, April C. Brown

Graduate Theses and Dissertations

Angus-sired (n = 30) and Hereford-sired (n = 32) bull calves were assigned randomly to 1 of 4 treatments. Treatments were 1) surgical castration near birth, 2) oral administration of the analgesic meloxicam (1 mg/kg of body weight) followed by surgical castration near birth, 3) surgical castration at weaning, or 4) surgical castration at weaning with oral administration of the analgesic meloxicam (1 mg/kg of body weight). Data was recorded for behavior, hematology, and growth at birth and weaning. Calf standing and lying activity was monitored at both stages by recording x and y axis positions of a data logger …

Influence Of Nutritional Modifications On Sow, Litter, And Nursery Performance, Benjamin Edward Bass

Graduate Theses and Dissertations

Two separate series of experiments were conducted to determine: 1) the influence of dietary arginine supplementation in late gestation on reproductive performance, and 2) the impact of a whole yeast product in gestation, lactation, and weaned pig diets on reproductive, growth, and immune parameters.

In the first study 99 sows were provided a control diet, or the control diet supplemented with 1% L-arginine from gestation d 93 to 110. Compared to control-fed sows, no differences were observed in reproductive parameters, however a tendency for increased gestational weight gain was observed for sows provided supplemental arginine.

In a second study, 98 …

Evaluation Of Internal Markers For Predicting Digestibility And Fecal Output By Cattle Fed Bermudagrass Hays Of Varying Quality, Juvenal Kanani

Graduate Theses and Dissertations

The potential of in situ rumen undegradable dry matter (RUDM), indigestible neutral-detergent fiber (INDF), indigestible acid-detergent fiber (IADF), acid-detergent insoluble ash (ADIA), alkaline-peroxide lignin (APL), and acid-detergent lignin (ADL) to predict digestibility (DMD) and fecal output (FO) by cattle fed bermudagrass [Cynodon dactylon (L.) Pers.] hay-diets categorized by their low (L), medium low (ML), medium high (MH), or high (H) CP concentrations (79, 111, 131, and 164 g/kg DM, respectively) was evaluated. The second objective was to evaluate the effects of time (0600, 1200, 1800, and 2400 h) of fecal sampling on the prediction of FO and DMD. A replicated …

Relationship Of Polymorphisms In The Fsh Beta Subunit Gene With Reproduction In Bos Taurus And Bos Indicus Cattle, Amanda Jo Davis

Graduate Theses and Dissertations

Two experiments were conducted to characterize polymorphisms (SNP) in the bovine FSHβ gene promoter region to examine breed differences in SNP, and to determine effects of SNP on reproduction in beef cattle. Experiment 1 - DNA samples were collected from 5 Angus (ANG), 13 Balancer (BAL), & 16 Brahman influenced (BI) bulls. Polymorphisms were identified by sequencing of 3 sequential PCR products from the promoter region revealed 17 SNP & 4 insertion/deletions (INDEL). Semen samples were collected and sperm quality variables determined via computer assisted sperm analysis (CASA) or stained smears. The MIXED procedure for ANG & BAL indicated interactions …

Stable Isotope Analysis (Delta Nitrogen-15 And Delta Carbon-13) And Bioenergetic Modeling Of Spatial-Temporal Foraging Patterns And Consumption Dynamics In Brown And Rainbow Trout Populations Within Catch-And-Release Areas Of Arkansas Tailwaters, Jon M. Flinders

Graduate Theses and Dissertations

I examined spatial and temporal consumption dynamics using an energy intake model and a bioenergetics model of rainbow trout, Oncorhynchus mykiss, and brown trout, Salmo trutta, within three catch-and-release (C-R) areas in Bull Shoals and Norfork tailwaters to determine whether trout populations were limited by food supply. I also examined the seasonal and ontogenetic shifts in the foraging patterns of brown and rainbow trout within these areas using gut content analysis (GCA) and stable isotope analysis (SIA) of C and N. I examined 605 brown trout and 768 rainbow trout for GCA and SIA at Bull Shoals, Norfork, …

Effect Of Supplemental Trace Mineral Source (Organic Versus Inorganic) On Bull Semen Quality, Matt Rowe

Graduate Theses and Dissertations

Studies indicate that organic forms of trace minerals can improve cow reproductive performance, particularly during periods of stress. However, limited information is available on the effects of organic trace mineral supplementation on bull fertility. The objective of this study was to evaluate the effect of organic versus inorganic trace mineral supplementation on bull semen quality before and after freezing, as measured by computer-assisted sperm analysis (CASA). Angus and Balancer bulls were assigned to inorganic (n = 9) and organic (n = 10) trace mineral treatments, based on initial semen quality, breed, body weight, and age. The bulls were maintained in …

Effects Of Ergot Alkaloids And Antioxidants On Bovine Sperm Motility, Ryan James Page

Graduate Theses and Dissertations

The studies that comprise this thesis were performed in an attempt to identify and eliminate stressful conditions that may lead to infertility in the male bovine. The first project was performed to determine if ergot alkaloids directly affect bovine sperm motility. Percentage of motile spermatozoa was affected (P = 0.015) by a three-way interactions between time, concentration, and alkaloid. Ergotamine and dihydroergotamine decreased (P = 0.01) sperm motility in a concentration and time dependant manner and ergonovine had a minimal effect on overall sperm motility. The number of static spermatozoa also was affected (P < 0.01) by a three way interaction and increased as ET and DHET concentrations increased. Percentages of progressively motile and rapidly motile spermatozoa decreased (P < 0.01) in a two way interaction between alkaloid and concentration. Overall, sperm motility was decreased by ET and DHET. Furthermore, the qualities of motility as represented by progressive, rapid, path velocity (VAP), progressive velocity (VSL), track speed (VCL), beat frequency (BCF), lateral amplitude (ALH), straightness (STR), elongated, and area were decreased by those alkaloids. These data verify that ergot alkaloids commonly found in toxic tall fescue are detrimental to bovine spermatozoa. In the second study two antioxidants (alpha-tocopherol and ascorbic acid) were added to bovine sperm culture media and cryopreservation extender. The antioxidant capabilities of these two vitamins could help reduce free radical production and help preserve sperm viability. There was an interactive effect between ascorbic acid concentration and storage method on the bovine sperm motility characteristics: motile, progressive, rapid, track speed (VCL), and straightness (P < 0.05). A bull by ascorbic acid interaction (P < 0.05) was observed for motile, progressive, rapid, path velocity (VAP), progressive velocity (VSL), and VCL characteristics. Alpha tocopherol had no affects on sperm motility characteristics. Lipid peroxidation was affected (P < 0.01) by bull and ascorbic acid. Malondialdehyde concentrations for ascorbic acid treatments (0, 5, 10, 20 mM) were 3.25, 4.2, 2.96, and 2.15 ìM respectively. Results from the second study indicate that the addition of ascorbic acid may reduce sperm motility, but help prevent lipid peroxidation.