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Full-Text Articles in Life Sciences
Characterization Of The Marine Sponge Amphimedon Compressa Microbiome Across A Spatial Gradient, Renee Michelle Potens
Characterization Of The Marine Sponge Amphimedon Compressa Microbiome Across A Spatial Gradient, Renee Michelle Potens
HCNSO Student Theses and Dissertations
Diverse and ecologically important microbial communities (microbiomes) are symbiotic within marine sponges. In this study, the microbiome of Amphimedon compressa from three sample locations (Broward and Dade Counties, Southeast Florida, USA and the Southern Caribbean, Bocas del Toro, Panama) is characterized using 16S rRNA Illumina sequencing. The predominant taxa are Proteobacteria and Cyanobacteria, as expected for Low Microbial Abundance sponges, accounting for over 53% of the total microbiome community. The numbers of Operational Taxonomic Units (OTUs) decrease from Broward County (2,900) to Dade County (2,300) and then Bocas del Toro (1,200). The correlates to a decreasing north-south gradient of …
Discrimination Of Species In The Montastrea Annularis Complex Using Multiple Genetic Loci, Jose V. Lopez, Nancy Knowlton
Discrimination Of Species In The Montastrea Annularis Complex Using Multiple Genetic Loci, Jose V. Lopez, Nancy Knowlton
Marine & Environmental Sciences Faculty Articles
Montastraea annularis, M. franksi and M. faveolata are a complex of recently distinguished coral species whose status remains controversial due to the lack of fixed differences. Here we report on two types of genetic analyses for these taxa: 1) DNA sequences of two nuclear genes [internal transcribed spacers of rDNA (ITS-1, ITS-2) and a ɮ-tubulin intron] and 2) a preliminary screening of the entire nuclear genome using amplified fragment length polymorphisms (AFLP). There was very little variation within or among the three species in DNA sequences. Onlly 3 of 300 nucleotide positions in ITS-1, 3 of the 350 positions …