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Full-Text Articles in Life Sciences

Small Scale Changes In Zooplankton Community In Relation To Stratification And Phytoplankton Thin Layers, Adam T. Greer Feb 2012

Small Scale Changes In Zooplankton Community In Relation To Stratification And Phytoplankton Thin Layers, Adam T. Greer

Adam T. Greer

Gelatinous zooplankton represent a poorly understood component of marine food webs in part because of difficulties estimating abundance using traditional net sampling techniques. The degree of vertical patchiness can influence the trophic impact of jellies, and the biophysical drivers of their distributions remain unknown. Using a towed In Situ Ichthyoplankton Imaging System (ISIIS), we examined the changes in jelly distributions during upwelling and relaxation events in Monterey Bay, CA. Sampling began at the end of a 12 d stretch of upwelling favorable winds. The first day of sampling was marked by a thin layer of high chlorophyll fluorescence and intense …


Science Boot Camp For Librarians: Cpd On A Shoestring, Maxine G. Schmidt, Rebecca Reznik-Zellen May 2011

Science Boot Camp For Librarians: Cpd On A Shoestring, Maxine G. Schmidt, Rebecca Reznik-Zellen

Maxine G Schmidt

Science Boot Camp for Librarians was envisioned as a casual but intensive immersion event into selected scientific subjects that employ networked computing capabilities for research and collaboration. The goal of the event is to provide librarians with networking opportunities, but more importantly, to give them some of the context and ocabulary of a discipline to enable them to better engage faculty and research scientists with regard to escience. A half-day is devoted to each of three topics chosen for that year’s camp. A local faculty member provides an overview of the research area, and a second describes a single project …


Niche Of Harmful Alga Aureococcus Anophagefferens Revealed Through Ecogenomics, Christopher Gobler, Dianna Berry, Sonya Dyhrman, Steven Wilhelm Jan 2011

Niche Of Harmful Alga Aureococcus Anophagefferens Revealed Through Ecogenomics, Christopher Gobler, Dianna Berry, Sonya Dyhrman, Steven Wilhelm

Steven Wilhelm

Harmful algal blooms (HABs) cause significant economic and ecological damage worldwide. Despite considerable efforts, a comprehensive understanding of the factors that promote these blooms has been lacking, because the biochemical pathways that facilitate their dominance relative to other phytoplankton within specific environments have not been identified. Here, biogeochemical measurements showed that the harmful alga Aureococcus anophagefferens outcompeted co-occurring phytoplankton in estuaries with elevated levels of dissolved organic matter and turbidity and low levels of dissolved inorganic nitrogen. We subsequently sequenced the genome of A. anophagefferens and compared its gene complement with those of six competing phytoplankton species identified through metaproteomics. …


Determining Rates Of Virus Production In Aquatic Systems By The Virus Reduction Approach,, M.G. Weinbauer, J.M. Rowe, Steven Wilhelm Jan 2010

Determining Rates Of Virus Production In Aquatic Systems By The Virus Reduction Approach,, M.G. Weinbauer, J.M. Rowe, Steven Wilhelm

Steven Wilhelm

The reduction approach to assess virus production and the prokaryotic mortality by viral lysis stops new infection by reducing total virus abundance (and thus virus–host contacts). This allows for easy enumeration of viruses that originate from lysis of already infected cells due to the decreased abundance of free virus particles. This reoccurrence can be quantified and used to assess production and cell lysis rates. Several modifications of the method are presented and compared. The approaches have great potential for elucidating trends in virus production rates as well as for making generalized estimates of the quantitative effects of viruses on marine …


The Construction And Analysis Of Marker Gene Libraries, S.M. Short, F. Chen, Steven Wilhelm Jan 2010

The Construction And Analysis Of Marker Gene Libraries, S.M. Short, F. Chen, Steven Wilhelm

Steven Wilhelm

Marker genes for viruses are typically amplified from aquatic samples to determine whether specific viruses are present in the sample, or to examine the diversity of a group of related viruses. In this chapter, we will provide an overview of common methods used to amplify, clone, sequence, and analyze virus marker genes, and will focus our discussion on viruses infecting algae, bacteria, and heterotrophic flagellates. Within this chapter, we endeavor to highlight critical aspects and components of these methods. To this end, instead of providing a detailed experimental protocol for each of the steps involved in examining virus marker gene …


E-Science @ Umass: Anticipating And Supporting E-Science Activities At The University Of Massachusetts, Maxine G. Schmidt, Rebecca Reznik-Zellen Jul 2009

E-Science @ Umass: Anticipating And Supporting E-Science Activities At The University Of Massachusetts, Maxine G. Schmidt, Rebecca Reznik-Zellen

Maxine G Schmidt

In March of 2008 an Ad Hoccommittee of Science Librarians from the University of Massachusetts Five Campus System convened to discuss the challenges of e-science and prepare the Libraries for their role in e-science initiatives. Three primary outcomes intended to support e-science activities emerged from the work of the Ad Hoc committee.


E-Science @ The University Of Massachusetts, Maxine G. Schmidt, Rebecca Reznik-Zellen, Raquel Rivera, Cecilia P. Mullen Mar 2009

E-Science @ The University Of Massachusetts, Maxine G. Schmidt, Rebecca Reznik-Zellen, Raquel Rivera, Cecilia P. Mullen

Maxine G Schmidt

e-Science @ the University of Massachusetts Abstract: What is e-Science and how can libraries and librarians support it? The University of Massachusetts takes a proactive approach to support network-enabled research on its campuses and provides examples where e-Science is already at work. Statement: “e-Science” is a term commonly used to describe research in a networked environment, a growing trend not only in the sciences, but the arts and humanities as well. e-Science creates both opportunities and challenges for academic libraries. The opportunities lie in leveraging the basic skill set that libraries and librarians already possess: the knowledge of and practical …


Actinorhodopsin Genes Discovered Diverse In Freshwater Habitats And Among Cultivated Actinobacteria, A.K. Sharma, K. Sommerfeld, G.S. Bullerjahn, A.R. Matteson, Steven Wilhelm, J. Jezbera, U. Brandt, W.F. Doolittle, M.W. Hahn Jan 2009

Actinorhodopsin Genes Discovered Diverse In Freshwater Habitats And Among Cultivated Actinobacteria, A.K. Sharma, K. Sommerfeld, G.S. Bullerjahn, A.R. Matteson, Steven Wilhelm, J. Jezbera, U. Brandt, W.F. Doolittle, M.W. Hahn

Steven Wilhelm

Microbial rhodopsins are membrane proteins that utilize a retinal chromophore to harvest sunlight for energetic and photosensory functions. Recently, a group of novel rhodopsin sequences named ‘actinorhodopsins’ (ActRs) was hypothesized to exist among uncultured planktonic Actinobacteria. ActRs were discovered by mining metagenomic data obtained during the Venter Institute’s Global Ocean Sampling expedition, from a hypersaline lagoon, two estuaries and a freshwater lake. On the basis of these findings, and many studies that show Actinobacteria are common inhabitants of lakes, we predicted that ActR genes would likely be present in other freshwater habitats and among the genomes of cultivated Actinobacteria …


Identifying The Source Of Unknown Microcystin Genes And Predicting Microcystin Variants By Comparing Genes Within Uncultured Cyanobacterial Cells, C.J. Allender, G.R. Lecleir, J.M. Rinta-Kanto, R.L. Small, M.F. Satchwell, G.L. Boyer, Steven Wilhelm Jan 2009

Identifying The Source Of Unknown Microcystin Genes And Predicting Microcystin Variants By Comparing Genes Within Uncultured Cyanobacterial Cells, C.J. Allender, G.R. Lecleir, J.M. Rinta-Kanto, R.L. Small, M.F. Satchwell, G.L. Boyer, Steven Wilhelm

Steven Wilhelm

While multiple phylogenetic markers have been used in the culture independent study of microcystin producing cyanobacteria, in only a few instances have multiple markers been studied within individual cells, and in all cases these studies have been conducted with cultured isolates. Here, we isolate and evaluate large DNA fragments (> 6 kb) encompassing two genes involved in microcystin biosynthesis (mcyA2 and mcyB1) and use them to identify the source of gene fragments found in water samples. Further investigation of these gene loci from individual cyanobacterial cells allowed for improved analysis of the genetic diversity within microcystin producers as …


Distribution Of Calcifying And Silicifying Phytoplankton In Relation To Environmental And Biogeochemical Parameters During The Late Stages Of The 2005 North East Atlantic Spring Bloom, K. Leblanc, C.E. Hare, Y. Feng, G.M. Berg, G.R. Ditullio, A. Neeley, I. Benner, C. Sprengel, A. Beck, S.A. Sanudo-Wilhemy, U. Passow, K. Klinck, J.M. Rowe, Steven Wilhelm, C.W. Brown, D.A. Hutchins Jan 2009

Distribution Of Calcifying And Silicifying Phytoplankton In Relation To Environmental And Biogeochemical Parameters During The Late Stages Of The 2005 North East Atlantic Spring Bloom, K. Leblanc, C.E. Hare, Y. Feng, G.M. Berg, G.R. Ditullio, A. Neeley, I. Benner, C. Sprengel, A. Beck, S.A. Sanudo-Wilhemy, U. Passow, K. Klinck, J.M. Rowe, Steven Wilhelm, C.W. Brown, D.A. Hutchins

Steven Wilhelm

The late stage of the North East Atlantic (NEA) spring bloom was investigated during June 2005 along a transect section from 45 to 66° N between 15 and 20° W in order to characterize the contribution of siliceous and calcareous phytoplankton groups and describe their distribution in relation to environmental factors. We measured several biogeochemical parameters such as nutrients, surface trace metals, algal pigments, biogenic silica (BSi), particulate inorganic carbon (PIC) or calcium carbonate, particulate organic carbon, nitrogen and phosphorus (POC, PON and POP, respectively), as well as transparent exopolymer particles (TEP). Results were compared with other studies undertaken in …


The Effects Of Increased Pco2 And Temperature On The North Atlantic Spring Bloom: I. The Phytoplankton Community And Biogeochemical Response, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, Y Zhang, G.R. Ditullio, P. Lee, Steven Wilhelm, J.M. Rowe, J. Sun, N. Nemcek, C. Gueguen, U. Passow, I. Benner, C. Brown, D.A. Hutchins Jan 2009

The Effects Of Increased Pco2 And Temperature On The North Atlantic Spring Bloom: I. The Phytoplankton Community And Biogeochemical Response, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, Y Zhang, G.R. Ditullio, P. Lee, Steven Wilhelm, J.M. Rowe, J. Sun, N. Nemcek, C. Gueguen, U. Passow, I. Benner, C. Brown, D.A. Hutchins

Steven Wilhelm

ABSTRACT: The North Atlantic spring bloom is one of the largest annual biological events in the ocean, and is characterized by dominance transitions from siliceous (diatoms) to calcareous (coccolithophores) algal groups. To study the effects of future global change on these phytoplankton and the biogeochemical cycles they mediate, a shipboard continuous culture experiment (Ecostat) was conducted in June 2005 during this transition period. Four treatments were examined: (1) 12°C and 390 ppm CO2 (ambient control), (2) 12°C and 690 ppm CO2 (high pCO2), (3) 16°C and 390 ppm CO2 (high temperature), and (4) 16°C and …


Effects Of Increased Pco2 And Temperature On The North Atlantic Spring Bloom: Iii. Dimethylsulfoniopropionate, P.A. Lee, J.R. Rudisill, A.R. Neeley, D.A. Hutchins, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, Steven Wilhelm, J.M. Rowe, G.R. Ditullio Jan 2009

Effects Of Increased Pco2 And Temperature On The North Atlantic Spring Bloom: Iii. Dimethylsulfoniopropionate, P.A. Lee, J.R. Rudisill, A.R. Neeley, D.A. Hutchins, Y. Feng, C.E. Hare, K. Leblanc, J.M. Rose, Steven Wilhelm, J.M. Rowe, G.R. Ditullio

Steven Wilhelm

The CLAW hypothesis argues that a negative feedback mechanism involving phytoplankton-derived dimethylsulfoniopropionate (DMSP) could mitigate increasing sea surface temperatures that result from global warming. DMSP is converted to the climatically active dimethylsulfide (DMS), which is transferred to the atmosphere and photochemically oxidized to sulfate aerosols, leading to increases in planetary albedo and cooling of the Earth’s atmosphere. A shipboard incubation experiment was conducted to investigate the effects of increased temperature and pCO2 on the algal community structure of the North Atlantic spring bloom and their subsequent impact on particulate and dissolved DMSP concentrations (DMSPp and DMSPd). Under …


Climate Change And Freshwater Resources, Noah D. Hall, Bret B. Stuntz, Robert H. Abrams Jan 2008

Climate Change And Freshwater Resources, Noah D. Hall, Bret B. Stuntz, Robert H. Abrams

Noah D Hall

The Earth’s climate is warming. This is the unequivocal conclusion of climate scientists. Despite the complexities of climatology, certain consistent trends emerge with implications for water availability: as the world gets warmer, it will experience increased regional variability in precipitation, with more frequent heavy precipitation events and more susceptibility to drought. These simple facts will have a profound impact on freshwater resources throughout the United States, as the warmer climate will reduce available water supplies and increase water demand. Unfortunately, current water law and policy are not up to the new challenges of climate change and resulting pressures on freshwater …


Climate Change And Carbon Sequestration: Assessing A Liability Regime For Long-Term Storage Of Carbon Dioxide, Alexandra B. Klass, Elizabeth J. Wilson Jan 2008

Climate Change And Carbon Sequestration: Assessing A Liability Regime For Long-Term Storage Of Carbon Dioxide, Alexandra B. Klass, Elizabeth J. Wilson

Alexandra B. Klass

As the nation struggles with how to address climate change, one of the most significant questions is how to reduce increasing levels of carbon dioxide in the atmosphere. One promising technology is carbon capture and sequestration (“CCS”), which consists of capturing carbon dioxide emissions from power plants and industrial sources and sequestering them in deep geologic formations for long periods of time. Areas for potential CO2 sequestration include oil and gas fields, saline aquifers, and coal seams. As Congress and the private sector begin to spend billions of dollars to research and deploy this technology, there has been insufficient attention …


The Hazardous Waste Land, Jerry L. Anderson Nov 1993

The Hazardous Waste Land, Jerry L. Anderson

Jerry L. Anderson

This article was one of the first comprehensive critiques of the Superfund remediation and liability system. The article addresses systemic problems with the CERCLA mechanism that result in inequity and slow the pace of cleanups.