Life Sciences Commons^™

Changes In Fruit And Vegetable Household Food Availability Among Low-Income Families Over A One-Month Period Of Time, Ann Alicia Wells

Theses and Dissertations

Objective: There is little evidence of how fruit and vegetable (FV) household food availability changes over a one-month period among low-income households. The objective of this study was to analyze how FV variety and sustainability changes over a 4-week period.
Design: Inventories were conducted in low-income family households (n=49) once a week over a 4-week period. Trained researchers gathered the weights of all FV, including legumes, within the home. Previously determined mean container weights were subtracted to obtain the estimated weight of the FV. All weights were then converted to edible cups of FV, taking into account the weight that …

Planning, Implementation, And Formative Evaluation Of A Food Literacy Program, Heather M. Thomas

Electronic Thesis and Dissertation Repository

The overall purpose of this dissertation was to examine the planning, implementation, and formative evaluation of a community-based food literacy program for youth. Article 1 provided a summary of the community-based cooking program for at-risk youth. Objectives included the provision of applied food literacy and cooking skills education augmented with fieldtrips to local farms. Eight at-risk youth (five girls and three boys, mean age = 14.6) completed the intervention. Post intervention, five of eight participants completed in-depth interviews about their experiences.

Article 2 was a formative evaluation of the cooking program focused on gaining an understanding of participants’ (i.e., at-risk …

A Measure Of Self-Efficacy Among Agency Directors To Offer Nutrition Education To Lowcountry Food Bank Clients, Lucie Maguire

All Theses

In 2005, the Lowcountry Food Bank (LCFB) in Charleston, SC, launched an initiative to provide nutrition education to clients that seek food assistance. The agency directors of the 332 member agencies of the LCFB are in direct contact with clients, therefore they could provide nutrition education rather than the LCFB staff alone. As such, the self-efficacy of agency directors to deliver nutrition education must be measured. The objective of this project was to measure the self-efficacy of agency directors of the LCFB. A 17-item instrument measuring self-efficacy was developed, including ten validated items from the General Self-Efficacy Scale and an …

Molecular Interactions Of Salmonella With The Host Epithelium In Presence Of Commensals, Prerak T. Desai

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Food-borne infections are a major source of mortality and morbidity. Salmonella causes the highest number of Food-borne bacterial infections in the US. This work contributes towards developing strategies to control Salmonella by (a) defining receptors used by Salmonella to adhere to and invade the host epithelium; (b) developing a host receptor based rapid detection method for the pathogen in food matrix; (C) and defining mechanisms of how probiotics can help alleviate Salmonella-induced cell death in the host epithelium.

We developed a cell-cell crosslinking method to discover host-microbe receptors, and discovered three new receptor-ligand interactions. Interaction of Salmonella Ef-Tu with …

Can Antioxidant-Rich Berries Improve Risk Factors Associated With Cardiovascular Disease In Postmenopausal Smokers?, Jordan Alaine Teeple

Graduate Theses and Dissertations

The purpose of this study was to examine the effects of smoking on risk factors associated with cardiovascular disease (CVD) and determine the efficacy of antioxidant-rich berries in ameliorating these risk factors in postmenopausal smokers. Menopause and cigarette smoking have been identified as major risk factors for CVD due to a decrease in antioxidant protection, increase in inflammation and oxidative stress, and adverse changes in serum lipids. Antioxidants, specifically flavonoid compounds found in blackberries and blueberries, have been shown to prevent low-density lipoprotein cholesterol oxidation and thus prevent downstream inflammation and oxidative stress. Healthy, postmenopausal nonsmokers (n=14) and smokers (n=31) …

Subcritical Water Hydrolysis Of Whey Proteins, Ashley Dawn Espinoza

Graduate Theses and Dissertations

Hydrolyzing food by-products is a unique approach to potentially increase by-product value and reduce waste. An abundant by-product of cheese production, whey, contains all essential amino acids and some distinctive peptides with functional and nutraceutical properties. Typically, proteins from whey are tailored for specific uses by chemical or enzymatic hydrolysis; however, subcritical water hydrolysis is a novel alternative used successfully to hydrolyze various substrates. Nevertheless, minimal research exists on: (1) the hydrolysis of whey protein; (2) the incorporation of additives; (3) the hydrolysis of whole whey; and (4) the production of volatiles when using subcritical water hydrolysis.

Therefore, whey protein …

Eat The Rainbow! An Evaluation Of A Short-Term Fruit And Vegetable Nutrition Education Intervention For Elementary School Children, Elizabeth Diane Miller

Masters Theses

Objective: To test differences on mean fruit and vegetable (FV) eaten, liking, preference, and self-efficacy scores among 3 modes of nutrition education intervention after a 3-week intervention.

Design: Convenience sample, pre- and post-test, quasi-experimental design.

Setting: Three elementary schools in a rural Eastern Tennessee County.

Participants: Participants were 160 3^rd-5^th graders.

Interventions: Three study schools: experiential (nutrition education, taste tests, and learning activity), conventional (nutrition education and learning activity), and control (learning activity).

Main Outcome Measures: Changes in pre- to post-intervention mean FV eaten, liking, preference, and self-efficacy scores.

Analysis: Mixed model ANOVA to compare the mean …

The Food Waste Management System In A Southeastern Hospital, Amal Habadi

All Theses

Effective waste management has always posed a challenge to those in food service operations. This study investigated the amount of food waste from Bon Secours St. Francis Health System foodservice operations using the Trim Trax¨ program. Trim Trax¨ is a food waste management program in which employees measure the volume of waste produced in pre-production and post-production areas. The objectives of this study were to collect the amount of waste produced during meal preparations and compare it with three weeks of retrospective data kept by employees in the salad/baking pre-production area, the cooking/grilling pre-production area and post-production area. Results showed …

Feeding Practices Of Mothers In The Gobi Desert Of Mongolia, Julie F. Tate

Electronic Theses and Dissertations

The diets of Mongolia infants are deficient in vitamins and minerals and complementary foods are introduced too early. Breastfeeding is the best source of nutrition for infants until 6 months of age. A survey of 50 Mongolian mothers with children under the age of 5 in the town of Sainshand, Dornogobi, was conducted in July 2011. Mongolian infants are among the highest percent of infants in the world who are exclusively breastfed for the first 6 months of their lives, thus providing them with excellent nutrition. However, complementary feedings and supplements are not appropriately used or understood. Therefore, the diets …

An Examination Of Diet, Acculturation And Risk Factors For Heart Disease Among Jamaican Immigrants, Carol Renee Oladele

USF Tampa Graduate Theses and Dissertations

Background: The South Florida region is home to over 85,000 Jamaican immigrants. Yet, little is known about the dietary intakes and predictors of risk of disease within this immigrant group. An assessment of dietary intakes and the development of dietary intake methodologies specific to the Jamaican population was important as it permitted accurate estimation of the nutrient intakes of this immigrant population whose dietary habits are not well documented. In addition, nothing is known about the prevalence of risk factors for heart disease or factors influencing risk factors among this immigrant group. The purpose of this study was to assess …

Maternal, Neonatal And Feeding Type Factors Associated With Severity Of Necrotizing Enterocolitis, Cheryl Ann Miner

Theses and Dissertations

Objective: To identify statistical associations with necrotizing enterocolitis (NEC) severity as dichotomized into cases with Bell stage II and III disease.
Study Design: We conducted a retrospective study using eight consecutive years of data from a multihospital healthcare system analyzed NEC severity (Bell stage II vs. III).
Results: We identified 220 neonates with stage ≥ II who had 225 separate episodes of NEC (157 stage II and 68 stage III). Those with stage III were born at earlier gestational age (P<0.0001) and lower birth weight (P<0.0001). Diagnosis of NEC occurred on about the same day of life in stage II and stage III cases. Those who developed stage III had significantly higher C-reactive protein (P<0.0001), I/T ratio (P= 0.0005), mean platelet volume (MPV) (P= 0.0001) and lower pH (P<0.0001) and platelet counts (P<0.0001). Transfusions were more common to those who progressed to stage III (P<0.0001). Regression analysis indicated higher odds of stage III in relationship to the volume of RBC transfusions (OR 2.41, {CI 1.85 to 3.11}, P<0.0001) and pasteurized human milk (PHM) (OR 1.32, {CI 1.07 to 1.62}, P = 0.0089). In contrast, feeding early mother's own milk (colostrum) for five days reduced the odds for stage III (OR 0.802, {CI 0.67 to 0.96}, P=0.0170). Those with small bowel resection were less likely to have received mother's own milk before NEC (OR 0.94, {CI 0.89 to 0.99}, P = 0.019) and factors predicting death from NEC were a low pH (OR 2.21, {CI 1.27 to 3.85}, P = 0.0005) and less colostrum (OR 0.96, {CI 0.94 to 0.99}, P = 0.003).
Conclusions: RBC transfusions and PHM increased the odds for stage III NEC, whereas early mother's own milk five days …

The Relationship Between Vitamin D And Calcium/Dairy Intake And Obesity In Children, Jason Barry

Nutrition Theses

Objective: The aim of this study is to examine the relationship between vitamin D and calcium intake and obesity in a population of young adolescents who participated in a Vitamin D and Sunlight Exposure study in Pittsburgh, PA.

Participants: 252 healthy 6 to 14.9 year old young adolescents (54% male, 69% African American) were recruited between June 2006 and December 2009.

Main outcome measures: Weight status, BMI, vitamin D intake, calcium intake, vitamin D and calcium rich food intake.

Results: A significant difference by race was observed with 30.1% of African Americans and 8.5% of Caucasians being obese (P<0.01). No difference was found by gender. Median (25%, 75%) vitamin D intake in the total population was 254.9 IU (146.8, 407.3) which is below the level recommended by the Institute of Medicine. Median calcium intake in the total population was 1193.6 mg (752.8, 1161.1) which met recommended guidelines. Median vitamin D intake differed by weight status (normal, overweight, obese) in the total population (259.5 IU, 325.2 IU and 181.9 IU, respectively; P=0.015). A similar pattern was observed for calcium (1193.4 mg, 1416.3 mg and 911.6 mg, respectively; P=0.016). No correlation was found between vitamin D or calcium intake and BMI in the total population or by race and gender.

Conclusion: …

An Eating Frequency Prescription For A Behavioral Weight Loss Intervention, Jessica Lynne Bachman

Doctoral Dissertations

Improved weight loss interventions are needed to help reduce obesity. One dietary factor that has been effective in increasing weight loss is increased dietary structure. One method for increasing dietary structure is prescribing the frequency in which eating bouts (meals and snacks) occur. Eating frequency (EF) has been inversely related to body mass index (BMI) but the impact of EF on weight loss is unclear. This randomized controlled trial examined the effect of EF on hunger, the relative-reinforcing value of food, energy intake (EI), and weight loss during a 6 month behavioral weight loss intervention. Participants (age: 51.0 ± 9.9 …

Distress Tolerance, Experiential Avoidance, And Negative Affect: Implications For Understanding Eating Behavior And Bmi, Christen Nicole Mullane

Doctoral Dissertations

Distress tolerance and experiential avoidance are important aspects of the coping process. In the current study, both were examined in relation to Body Mass Index and self-reported disturbances in mood and eating behavior. Distress tolerance was measured behaviorally and via self-report to elucidate the manner in which a) the ability to tolerate emotional distress, and b) the ability to persist behaviorally in the presence of stress-inducing stimuli were related to self-reported levels of depression, anxiety, maladaptive eating habits, and bodily concerns. A sample of 73 undergraduate students participated, and height, weight, and waist circumference were measured. Increased experiential avoidance was …

Genetic And Nutritional Studies To Elucidate The Role Of Adipose Tissue In The Pathogenesis Of Metabolic Syndrome, Nishan Sudheera Kalupahana

Doctoral Dissertations

Obesity is a major health problem in the United States and worldwide. It increases the risk for type-2 diabetes and cardiovascular diseases. A chronic low-grade inflammation occurring in white adipose tissue (WAT) is causally linked to the development of insulin resistance (IR), metabolic syndrome and obesity-associated chronic diseases. The aim of this dissertation research was to elucidate the WAT function in metabolic syndrome using genetic (overexpression of an adipose pro-inflammatory hormone, angiotensinogen) and nutritional manipulations/approaches (caloric restriction and omega-3 fatty acids), with specific emphasis on the role of inflammation.

Previous research indicates that WAT renin-angiotensin system (RAS) is overactivated in …

Measuring The Impact Of Youth Efnep: Questionnaire Development And Validation, Yenory Hernandez

All Dissertations

Background: Given the problem of childhood obesity and food insecurity among low-income children, the Youth Expanded Food and Nutrition Education Program (EFNEP) needs an appropriate, valid and reliable evaluation tool to determine the program effectiveness.
Purpose: To describe the development and preliminary validation of EFNEP Youth Quest questionnaire, an impact assessment tool designed for Youth EFNEP program.
Use of theory: The Community Nutrition Education logic model adapted with constructs of Social Cognitive Theory and Theory of Planned Behavior.
Targeted audience: Low income-ethnically diverse children, in third, fourth and fifth grade.
Design: The development of the questionnaire included six phases: preliminary …

A Sensory-Based Multi-Component School-Based Nutrition Intervention Among Fifth-Grade Students, Stacy Lyn Bevan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The nutritional status of children is declining as evidenced by the steady rise in childhood obesity rates over the last three decades. Consuming five servings of fruit and vegetables (FV) daily has shown to help with weight maintenance, but children are consuming far less than the recommended servings. This study was designed to test the efficacy of a sensory-focused multi-component school-based program at increasing vegetable intake among fifth-graders. Classroom, family, and community components allowed children to explore thirteen target vegetables with their senses including taste. Vegetable consumption was measured by digitalized observations of lunchtime vegetable selection and consumption. Vegetable acceptance …

Evaluation Of Levulinic Acid For Topical Decontamination Of Meat Surfaces, Jeffrey V. Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Experiments were performed to investigate the effects of wash treatments, consisting of hot water, 2% lactic, 2% acetic, or 2% levulinic acid, for decontamination of pathogenic bacteria previously inoculated onto meat surfaces, to inhibit growth of pathogenic bacteria inoculated onto previously washed meat surfaces, and on the organoleptic quality of sliced turkey roll and beef trim. Acid washes were no more effective at reducing Escherichia coli O157:H7 on beef plate, Listeria monocytogenes on sliced turkey roll, and Salmonella on pork belly than was water wash. Only lactic acid treatment was more effective than water at reducing Salmonella on chicken skin, …

Characterization Of Volatile And Metabolite Compounds Produced By Lactococcus Lactis In Low-Fat And Full-Fat Cheddar Cheese Extract, Michael J. Young

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted to compare and contrast potential aroma compounds in the headspace and small molecule metabolites produced as a result of starter culture metabolism in a full-fat and low-fat cheddar cheese model system. Past studies have indicated differences in the headspace flavor compound profiles between full-fat and low-fat Cheddar cheeses with no indication as to what compounds were produced as a result of starter culture metabolism.

Starter cultures were incubated in a Cheddar cheese extract environment that was made up of the water-soluble portion of Cheddar cheese with environmental conditions mimicking full-fat and low-fat Cheddar cheese by altering …

Effect Of Adjunct Cultures, Sodium Gluconate, And Ripening Temperature On Low-Fat Cheddar Cheese Flavor, Rebekah M. Lance

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Low-fat Cheddar cheese flavor is different from full-fat Cheddar cheese and is not acceptable to many consumers. This 2-part experiment was designed to examine effects adjunct cultures have on low-fat Cheddar cheese flavor as determined through descriptive analysis and consumer feedback.

In Part 1, low-fat (5%) Cheddar cheese was produced in duplicate, using 6 combinations of DVS850, LH32, CR540, CRL431, Emfour, and CR319 bacterial cultures. Due to a previously observed positive effect by sodium gluconate on low-fat cheese flavor, each replicate was split into treatments of 0.0% and 0.8% sodium gluconate. Each of these treatments was then split into ripening …

A Formative Evaluation For A Food Choices Intervention In A Mississippi Delta Community: A Focus Group Of Rural African American Adolescents, Jovan Chantrell Eugene Williams

Master's Theses

A needs assessment should be conducted to plan effective interventions. This study used a qualitative research approach to examine the eating patterns, food purchasing and preparation skills and practices, barriers and facilitators of dietary change, social and contextual influences on food choices, and preferences for communication and presentation channels and styles of low-income rural African American adolescents. Seven focus groups were conducted with a total of 33 participants, 21 girls and 12 boys, aged 13-18. Adolescents were grouped by age and gender. A moderator, who was trained in focus group methodology, conducted the session using a focus group discussion guide …

Nutritional Ergogenic Aids: The Influences Of Carbohydrate-Protein Supplementation During Endurance Exercise, Adriana Marina Coletta

Masters Theses

Mixed results, in terms of performance benefits, have been found when comparing carbohydrate (CHO) and carbohydrate-protein (CHO-P) supplementation during endurance exercise. Thus this study assessed performance from three different supplements (CHO-P, CHO, double carbohydrate [CHO-CHO]) as compared to a placebo (PLA) during a time trial (TT) run. Twelve male recreational runners (age = 32.4 ± 9.5 yrs; body mass index [BMI] = 22.7 ± 1.5 kg/m2; VO2max = 59.68 ± 7.53 mL/kg body weight; 100% white) individually completed four, 12-mile TT runs, 7-10 days apart, at about 75% of the their race pace. Dietary and physical activity consistency within the …

Angiotensin Iv And The Molecular Mechanisms Involved In The Development Of Insulin Resistance In 3t3-L1 Adipocytes, Julie Anne Jungwirth

Masters Theses

This study explored angiotensin IV’s (Ang IV) affects on the signaling pathways involved in the development of insulin resistance in 3T3-L1 adipocytes. Ang IV, working through the AT₄ receptor, interferes with insulin signaling through the blockade of the phosphatidylinositol 3-kinases (PI3K)/Akt pathway and through activating mitogen activated protein kinases (MAPK): extracellular signal regulated kinase (ERK) and c-Jun-N-terminal kinase (JNK) which are known to impair insulin receptor substrate-1 (IRS-1) signaling. The expression of AT₄ receptors was confirmed by reverse transcription polymerase chain reaction. Ang IV’s effects were found by treating adipocytes with combinations of Ang IV, AT …

The Relationship Between Carbohydrate Restrictive Diets And Body Fat Percentage In The Female Athlete, Lauren L. Lorenzo

Nutrition Theses

Purpose: To assess the dietary intake and body composition of recreational and competitive female athletes, for the purpose of analyzing the relationships between macronutrient intake and body composition.. The main aim was to determine the relationship between caloric intake, carbohydrate (CHO) intake and protein intake with body fat percentage in active females. Methods: Using an IRB approved protocol, 44 volunteer female recreational and competitive athletes 18 years of age or older were recruited. Interviews were conducted to gather information on within day energy balance by assessing the time and amount of foods/beverages consumed, and the duration and intensity (using a …

The Nutrition Environment Measurements Survey: An Assessment Of The Vending Machine Food And Drink Environment At Georgia State University, Ashley Depriest

Nutrition Theses

Purpose: Vending machines are a component of the food environment that influences dietary choices. Previous vending machine studies have focused on schools and work sites. The purpose of this study was to utilize the Nutrition Environment Measurements Survey-Vending (NEMS-V) online tool to evaluate and rank the nutritional value of the vending environment of a large urban university.

Methods: A sample size of 40 vending machines at Georgia State University (GSU) was chosen. A list of products in each machine was recorded and given either a red, yellow or green ranking based on their nutrient content. Finally, the NEMS-V online tool …

Teacher Practices, Attitudes, And Perceptions About The Decatur Farm To School Program, Ana Kucelin

Nutrition Theses

Purpose: Farm to School (F2S) Programs are increasing in number across the United States, yet research on teachers’ perceptions and practices about F2S is limited. Teachers are a key component of any F2S program, since their acceptance and support influences program implementation and sustainability. The purpose of this study was to examine teacher practices, attitudes, and perceptions about the Decatur F2S Program.

Methods: City Schools of Decatur teachers completed a questionnaire designed to obtain information concerning their practices, attitudes, and perceptions regarding the Decatur F2S Program. Participants indicated their level of agreement with 18 statements using a Likert scale rating, …

The Regulation Of Liv-1 Mrna In Mda-Mb-231 Human Breast Cancer Cells And Its Association With E-Cadherin (Cdh1), Leelyn Chong

Master's Theses

LIV-1, the estrogen-regulated gene encoding a member of the ZIP family of zinc transporters (SLC39A6), has been implicated in both growth and metastasis of estrogen-receptor (ER) positive (+) and negative (-) breast cancer cells. In ER⁺ breast cancer cells, LIV-1 expression is induced by both estrogen and insulin. In embryonic cells, LIV-1 has also been negatively associated with the expression of the cell adhesion protein, E-cadherin (CDH1), thought to play a role in metastasis. In the present study, we investigated the regulation of LIV-1 and its relationship to CDH1 in MDA-MB-231 ER^- breast cancer cells. Cells were …

The Effect Of Processing Parameters And Methylcellulose On Texture And Consumer Acceptability Of A Non-Melting Dairy Protein Gel, Joshua Goldman

Master's Theses

The overall goal was to understand the capabilities of a dairy based meat alternative. This was done in three phases: 1) the production of a dairy protein gel as a base for a dairy based meat alternative, 2) texturization of the dairy protein gel base to produce a nugget like texture, and 3) a consumer test to determine the acceptability of the dairy based nugget compared to meat and meat alternatives on the market.

For phase I, a dairy protein gel base was tested to understand the textural attributes. Milk to whey percentages and level of acidification were examined to …

A High Fiber Diet Versus A Low-Carbohydrate Diet For Weight Loss In Obese Individuals With Or Without Type 2 Diabetes, Neal G. Malik

Loma Linda University Electronic Theses, Dissertations & Projects

In the United States, obesity rates have climbed steadily over the years. Many different diets for weight loss have been proposed and studied, yet no one diet seems to clearly be more beneficial. Among diets that have been promoted are those high in fiber and low in carbohydrates, yet thus far, results of these studies have been mixed.

Using a 2x2 complete factorial design, 173 obese men and women with or without type 2 diabetes were randomized to one of two conditions: (a) a high fiber diet (> 40 grams of fiber per day for women, >50 grams of fiber …

Optimizing Micronutrient Intake Of Lactating Women In Kwazulu-Natal, South Africa, Through Increased Wild Edible Plant Consumption, Kerry Pearson

Master's Theses

OPTIMIZING MICRONUTRIENT INTAKE OF LACTATING WOMEN IN KWAZULU-NATAL, SOUTH AFRICA, THROUGH INCREASED WILD EDIBLE PLANT CONSUMPTION

Kerry Pearson

Micronutrient consumption in KwaZulu-Natal, South Africa, is insufficient to meet the needs of lactating women. Inadequate intake negatively impacts the health of both the mother and infant. Increasing consumption of wild edible plants has been recommended to combat these deficiencies, but information has not yet been provided on which types of wild plants should be eaten or in what quantity. The objective of this study was to determine the optimal mixture of wild edible plants that needs to be consumed to meet …