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Adapting The Auxin-Inducible Degron (Aid) System To The Zebrafish Model Organism, Dennis Li
Adapting The Auxin-Inducible Degron (Aid) System To The Zebrafish Model Organism, Dennis Li
Library/Life Sciences Undergraduate Poster Competition 2024
The Auxin Inducible Degron (AID) system is a conditional and reversible protein degradation system naturally occurring in plants. In the presence of the hormone Auxin, the AID tagged proteins are recruited into the plant’s E3 ubiquitin ligase machinery, ubiquitinated, and marked for degradation1. To adapt the AID system to the zebrafish model organism, our lab generated lines co-expressing Arabidopsis Thaliana plant TIR1 protein and GFP tagged with AID (GFP-AID) in superficially-located mechanosensory hair cells (HC) of the lateral line system and in spinal cord neurons found deeper in the body. Upon auxin application, GFP-AID expressed in the superficial HCs was …