Life Sciences Commons^™

In Situ Plant Uptake Of Excess Nutrients And Consequential Alteration Of Rhizosphere Dynamics, Srusti Maddala

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

The use of phytoremediation in ecological remediation projects has numerous benefits including soil stabilization and nutrient uptake. Recently, microdialysis, a diffusion-based sampling technique commonly used in biomedical research, has been recognized as a candidate for monitoring chemical changes in the rhizosphere. The real-time, in situ data it provides about nutrient diffusion may improve the management and success of restoration projects. Therefore, the objective of this study was to employ the technique of microdialysis in the novel application of quantifying the diffusive flux of inorganic nitrogen compounds in the rhizosphere of native plants of Arkansas. The microdialysis technique was first optimized …

Microdialysis: A Method For Quantifying In Situ Nitrogen Fluxes In Soil Microsites, Srusti Maddala, Mary C. Savin, Julie A. Stenken, Lisa S. Wood

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Microdialysis, a diffusion-based sampling technique commonly used in biomedical research, has recently been recognized as a candidate for monitoring chemical changes in the rhizosphere. The information it provides about nutrient diffusion may improve nitrogen use efficiency, leading to enhanced management and success of restoration projects. The objective of this study was to determine the efficacy of microdialysis sampling to quantify the relative recoveries (RR%) of nitrate-N and ammonium-N, the two inorganic nitrogen compounds typically found in soil. The effects of microdialysis flow rate, sample medium concentration, and the presence of both analytes in solution on the relative recoveries obtained from …

Strategies To Improve Forage Utilization By Sheep Offered Forage Mixtures, Valens Niyigena

Graduate Theses and Dissertations

In the first experiment, alfalfa and novel endophyte-infected tall fescue (NE+) forages were harvested after a killing frost, then mixed to formulate 4 different treatments; alfalfa alone, 67% alfalfa +33% fescue, 33% alfalfa + 67% fescue, or 100% fescue. After 3 months of storage as silage, Dorper ewe lambs (n = 20; mean BW = 34.7 ± 6.65 kg) were fed silage for ad libitum consumption, using 5 animals per treatment. Increasing the proportion of NE+ tall fescue improved (P < 0.05) silage total acids and lactic acid concentrations and decreased silage ammonia concentration. Digestible dry matter and organic matter intake and nitrogen utilization parameters decreased with increasing inclusion of NE+ in diet. In the second experiment, 16 Dorper ewe lambs (41.8 ± 4.61 kg BW) were assigned to 4 different treatments; alfalfa silage alone (0 g/kg; CONT) or alfalfa silage mixed with chopped sericea lespedeza (SL) hay to provide 90 (LOW), 180 (MED), or 270 g/kg SL (HIGH) on a dry matter basis in a randomized complete block design experiment with 2 period to provide 4 observations per treatment for each experimental period. Increasing the proportion of sericea lespedeza in the diet decreased dry matter and organic matter digestibility but did not affect feed intake. Fecal nitrogen (g/kg N intake) increased linearly (P < 0.01) while urinary N (g/ day and g/ kg of N intake) tended to decreased linearly and quadratically (P ≤ 0.1) with increasing the proportion of SL in diet. In the third experiments, 16 gestating Dorper ewe lambs (49.1 ± 4.61 kg BW) were allocated to 4 treatments; alfalfa silage alone (0% g/kg; CONT) or alfalfa silage mixed with lablab purpureus(LP) hay to provide 90 (LOW), 180 (MED), or 270 g/kg LP (HIGH) on a dry matter (DM) basis, in a randomized complete block design experiment with a total 8 replication per treatment. Supplementation of LP in diet increased quadratically (P ≤ 0.04) forage dry matter, organic matter intake, digestible dry matter and digestible organic matter intake (P < 0.05). Nitrogen apparently absorbed and urinary N both decreased linearly (P < 0.01) with adding more LP hay in diet. Harvesting and mixing alfalfa and fescue after a killing frost improved silage fermentation characteristics and supplementation of tannins from SL and polyphenol from LP altered N excretion.

Key words: lablab, sericea lespedeza, alfalfa silage, nitrogen, sheep.

Short-Term Denitrification In The Metalimnion Of A Eutrophic Reservoir, Aki Kogo, Erin M. Grantz, Thad Scott

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Denitrification in metalimnetic water was examined under different conditions to determine how addition of nitrate (NO3 -) and particulate carbon (PC), aeration, and concentrations of nitrate affect denitrification. In the first experiment, water samples from a metalimnion were treated with different combinations of NO3 - and PC. Changes in excess N2 -N concentration for 10 days were measured using membrane inlet mass spectrometry (MIMS). The second experiment used the same treatments as the first experiment after aeration of water. Besides excess N2 -N concentrations, O2 concentrations were measured for 16 days. The third experiment examined how different initial concentrations of …