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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Pathway-Specific Signaling And Its Impact On Fertility: A Focus On The Kisspeptin Receptor, Kiss1r, Maryse R. Ahow
Pathway-Specific Signaling And Its Impact On Fertility: A Focus On The Kisspeptin Receptor, Kiss1r, Maryse R. Ahow
Electronic Thesis and Dissertation Repository
Hypothalamic GnRH-releasing hormone (Gnrh) is the master regulator of the neuroendocrine reproductive axis and its secretion is regulated by many Gnrh neuronal-based signaling systems. Among these are Gαq/11-coupled receptors and their ligands.In most cases examined to date, activation of these receptors lead to Gnrh neuronal membrane depolarization and Gnrh secretion. The most potent trigger of Gnrh secretion is kisspeptin (Kp), a ligand for the Kiss1r Gαq/11-coupled receptor. Studies have shown that Kiss1r signaling is essential for attaining and maintaining fertility. We recently demonstrated that in addition to signaling via Gαq/11, Kiss1r also couples to …
The Role Of The Rna-Binding Protein Rho Guanine Nucleotide Exchange Factor In The Cellular Stress Response, Kevin Wh Cheung
The Role Of The Rna-Binding Protein Rho Guanine Nucleotide Exchange Factor In The Cellular Stress Response, Kevin Wh Cheung
Electronic Thesis and Dissertation Repository
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease for which the pathological mechanism is heterogeneous and a cure has been elusive. Recent developments have linked specific proteins found in pathological neuronal cytoplasmic inclusions (NCIs) of ALS motor neurons to familial variants of the disease. These proteins, including TAR DNA-binding protein of 43 kDa (TDP-43), fused in sarcoma/translocated in liposarcoma (FUS), and Rho guanine nucleotide exchange factor (RGNEF) share the common characteristic of being RNA-binding proteins that colocalize within NCIs. RGNEF is unique however in also possessing RhoA activation capacity, suggesting a role in the cell stress response. My thesis …
Characterization Of Human 82-Kda Choline Acetyltransferase Expression In A Newly Developed Transgenic Mouse Model, Silke M. Vanvaerenbergh
Characterization Of Human 82-Kda Choline Acetyltransferase Expression In A Newly Developed Transgenic Mouse Model, Silke M. Vanvaerenbergh
Electronic Thesis and Dissertation Repository
Expression of human 82-kDa choline acetyltransferase (ChAT) protein in nuclei of cultured neurons from Alzheimer’s disease model APP/PS1 transgenic mice results in a reduction in Aβ release, suggesting a protective role. This thesis characterizes the expression of human 82-kDa ChAT in 82-hChAT;NkCre transgenic mice by PCR-based examination of genomic DNA and localization of the protein in mice brains. First, I demonstrate in a cultured cell model that Cre recombinase-mediated excision of a floxed LacZ gene is necessary for human 82-kDa ChAT protein expression from pcCALL2:82-ChAT, the plasmid used to create founder transgenic mice. Second, I confirmed that human 82-kDa ChAT …
Synaptic Architecture Of The Acoustic Startle Response Pathway, Mahabba Smoka
Synaptic Architecture Of The Acoustic Startle Response Pathway, Mahabba Smoka
Electronic Thesis and Dissertation Repository
The acoustic startle response (ASR) is mediated by a simple pathway which includes the giant neurons of the caudal pontine reticular nucleus (PnC). Habituation is theorized to occur via hyperpolarizing big potassium (BK) channels localized at glutamatergic terminals of auditory afferents in the PnC. Prepulse inhibition is suggested to be mediated by cholinergic innervation of PnC giant neurons, with possible glutamate and/or GABA co-release. Animals were injected with Fluorogold at C3/C4 to label a subpopulation of PnC giant neurons, and following a startle experiment, brainstems were processed for pCREB expression. Using their respective markers, BK channels, glutamatergic, GABAergic, and cholinergic …
Sox9 Conditional Knockdown Reduces Chondroitin Sulphate Proteoglycan Expression, Increases Neuroplasticity, And Improves Motor Function In A Mouse Model Of Spinal Cord Injury, William M. Mckillop
Sox9 Conditional Knockdown Reduces Chondroitin Sulphate Proteoglycan Expression, Increases Neuroplasticity, And Improves Motor Function In A Mouse Model Of Spinal Cord Injury, William M. Mckillop
Electronic Thesis and Dissertation Repository
This thesis investigates the effect of Sox9 knockdown on anti-regenerative scar gene expression, neuroplasticity, and hind limb functional recovery following mouse spinal cord injury. We hypothesized that Sox9 knockdown would reduce expression of anti-regenerative chondroitin sulfate proteoglycans both at the lesion site and at sites distant to the injury, thus providing an avenue for increased neuroplasticity and locomotor recovery after spinal cord injury. The first chapter provides a general introduction to the biological problem of spinal cord injury. The development of the glial scar and expression of the anti-regenerative chondroitin sulfate proteoglycan extracellular matrix is introduced, and Sox9 is identified …