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Multispecies Genomic Sex Identification Using Ddx3 Gene Polymorphisms, Jessica Felts
Multispecies Genomic Sex Identification Using Ddx3 Gene Polymorphisms, Jessica Felts
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
PCR sex determination assays must be reliable and cost effective due to the frequent and integral use of these assays in biological research and the animal production industry. Thus, the design of proof of a primer pair with a built-in control is warranted to not only bypass the extra cost of a multiplex reaction, but also to prevent anomalous results that have been documented with other primer pairs.
The objective of this study was to design primer pairs with built in PCR amplification control to identify sex in Equus caballus (domestic horse), Homo sapiens (humans), Macaca mulatta (rhesus macaque), and …