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Characterization Of Afn1, A Gene Associated With Cereal Grain Germination, Tenzin Tsewang, Russell Johnson
Characterization Of Afn1, A Gene Associated With Cereal Grain Germination, Tenzin Tsewang, Russell Johnson
Undergraduate Research Symposium (UGRS)
The AFN1 gene is transiently expressed in germinating oat grains. As AFN1 is not expressed in dormant oat grains during imbibition, we hypothesize that AFN1 may be involved in stimulating the germination process. Sequence analysis of an AFN1 cDNA clone indicates that the AFN1 polypeptide is similar to a previously identified abscisic acid (ABA) glucosyl transferase. This suggests that AFN1 may be acting to glucosylate ABA, thereby inactivating it. As the hormone ABA is known to inhibit germination, ABA glucosylation/inactivation could lead to germination in grains expressing AFN1. To test this hypothesis, we have constructed an expression plasmid that encodes …