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Full-Text Articles in Life Sciences

Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake Aug 2020

Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake

Student Theses

Effective DNA extraction methods are important for forensic applications. The main goal of this experiment was to determine if a newly developed trypsin based protein/DNA co-extraction method applied to contact traces would yield comparable results to a commercial Proteinase K method (QIAamp DNA Investigator Kit) and a Chelex extraction method with Tween 20 used in forensic laboratories. This was tested on 20 sets of sebaceous fingerprints on glass slides.

The results of this study demonstrate the trypsin co-extraction method yielded the highest amount of DNA. In the first comparison, the mean total DNA yields for the trypsin-co extraction method and …


A Proteomic Analysis Of Corydoras Sterbai Secretions And Tissues, Erik Powell Wictor Jan 2020

A Proteomic Analysis Of Corydoras Sterbai Secretions And Tissues, Erik Powell Wictor

University of the Pacific Theses and Dissertations

Defensive mechanisms vary widely in the animal kingdom ranging from physical defenses like spines to chemical defenses such as toxins. Toxins in these secretions and tissues can fluctuate from enzymes to lipids to uncharacterized chemicals. Next generation -omics technology and mass spectrometry are extremely important in analyzing these samples because of their ability to distinguish minute amounts of toxic substance within a complicated sample. The goal of this experiment was to look at secretions and tissues from Corydoras sterbai. All samples in this study were proteolyzed using a mixture of Trypsin and Lys-C, fractionated, and run through nanoLC-MS/MS analysis using …