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Expression And Purification Of Human Lysosomal Β-Galactosidase From Pichia Pastoris, Sarah E. Tarullo
Expression And Purification Of Human Lysosomal Β-Galactosidase From Pichia Pastoris, Sarah E. Tarullo
Masters Theses
Lysosomal storage diseases are genetically inherited diseases caused by the dysfunction of lysosomal enzymes. In a normal cell, lysosomal enzymes cleave specific macromolecules as they are transported to the lysosome. However, in diseased cells, these lysosomal enzymes are either absent or malfunctioning, causing macromolecular substrates to accumulate, becoming toxic to the cell. Over fifty lysosomal storage diseases have been identified, collectively occurring in one out of 7,700 live births. We investigated the lysosomal enzyme β-galactosidase (β-gal). In order to study the biochemistry and enzymology of this protein a robust expression system was needed. The GLB1 gene has been inserted into …
Designing Protein Expression And Purification Systems For Recombinant Z Alpha1-Antitrypsin Using The Methylotrophic Yeast, Pichia Pastoris, Monique Jenaie Lemieux
Designing Protein Expression And Purification Systems For Recombinant Z Alpha1-Antitrypsin Using The Methylotrophic Yeast, Pichia Pastoris, Monique Jenaie Lemieux
Masters Theses
It is well established that improper protein folding often leads to the formation of aggregates whose consequences are cellular impairment and cell death. One example of this is the aggregation of the mutant Z Alpha1-Antitrypsin protein, which results in blocking of its secretion due to inclusion body formation. This can contribute not only to the development of chronic obstructive pulmonary disease but also to hepatitis, cirrhosis and hepatocellular carcinoma. Current treatments are principally limited to intravenous Alpha1-Antitrypsin therapy and organ transplantation. In the scientific community though, it is widely thought that more effective forms of treatments lie within the polymerization …