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Full-Text Articles in Life Sciences
A Rhizosphere Fungus Enhances Arabidopsis Thermotolerance Through Production Of An Hsp90 Inhibitor, Catherine A. Mclellan, Thomas J. Turbyville, E. M. Kithsiri Wijeratne, Arthur Kerschen, Elizabeth Vierling, Christine Queitsch, Luke Whitesell, A. A. Leslie Gunatilaka
A Rhizosphere Fungus Enhances Arabidopsis Thermotolerance Through Production Of An Hsp90 Inhibitor, Catherine A. Mclellan, Thomas J. Turbyville, E. M. Kithsiri Wijeratne, Arthur Kerschen, Elizabeth Vierling, Christine Queitsch, Luke Whitesell, A. A. Leslie Gunatilaka
Elizabeth Vierling
The molecular chaperone HEAT SHOCK PROTEIN90 (HSP90) is essential for the maturation of key regulatory proteins in eukaryotes and for the response to temperature stress. Earlier, we have reported that fungi living in association with plants of the Sonoran desert produce small molecule inhibitors of mammalian HSP90. Here, we address whether elaboration of the HSP90 inhibitor monocillin I (MON) by the rhizosphere fungus Paraphaeosphaeria quadriseptata affects plant HSP90 and plant environmental responsiveness. We demonstrate that MON binds Arabidopsis (Arabidopsis thaliana) HSP90 and can inhibit the function of HSP90 in lysates of wheat (Triticum aestivum) germ. MON treatment of Arabidopsis seedlings …
A High-Performance, Small-Scale Microarray For Expression Profiling Of Many Samples In Arabidopsis-Pathogen Studies, Masano Sato, Raka M. Mitra, John Coller, Dong Wang, Natalie W. Spivey, Julia Dewdney, Carine Denoux, Jane Glazebrook, Fumiaki Katagiri
A High-Performance, Small-Scale Microarray For Expression Profiling Of Many Samples In Arabidopsis-Pathogen Studies, Masano Sato, Raka M. Mitra, John Coller, Dong Wang, Natalie W. Spivey, Julia Dewdney, Carine Denoux, Jane Glazebrook, Fumiaki Katagiri
Dong Wang
Studies of the behavior of biological systems often require monitoring of the expression of many genes in a large number of samples. While whole-genome arrays provide high-quality gene-expression profiles, their high cost generally limits the number of samples that can be studied. Although inexpensive small-scale arrays representing genes of interest could be used for many applications, it is challenging to obtain accurate measurements with conventional small-scale microarrays. We have developed a small-scale microarray system that yields highly accurate and reproducible expression measurements. This was achieved by implementing a stable gene-based quantile normalization method for array-to-array normalization, and a probe-printing design …
Mutant Α-Galactosidase A Enzymes Identified In Fabry Disease Patients With Residual Enzyme Activity: Biochemical Characterization And Restoration Of Normal Intracellular Processing By 1-Deoxygalactonojirimycin, Satoshi Ishii, Hui-Hwa Chang, Kunito Kawasaki, Kayo Yasuda, Hui-Li Wu, Scott Garman, Jian-Qiang Fan
Mutant Α-Galactosidase A Enzymes Identified In Fabry Disease Patients With Residual Enzyme Activity: Biochemical Characterization And Restoration Of Normal Intracellular Processing By 1-Deoxygalactonojirimycin, Satoshi Ishii, Hui-Hwa Chang, Kunito Kawasaki, Kayo Yasuda, Hui-Li Wu, Scott Garman, Jian-Qiang Fan
Scott Garman
Fabry disease is a lysosomal storage disorder caused by the deficiency of alpha-Gal A (alpha-galactosidase A) activity. In order to understand the molecular mechanism underlying alpha-Gal A deficiency in Fabry disease patients with residual enzyme activity, enzymes with different missense mutations were purified from transfected COS-7 cells and the biochemical properties were characterized. The mutant enzymes detected in variant patients (A20P, E66Q, M72V, I91T, R112H, F113L, N215S, Q279E, M296I, M296V and R301Q), and those found mostly in mild classic patients (A97V, A156V, L166V and R356W) appeared to have normal K(m) and V(max) values. The degradation of all mutants (except E59K) …
A Specific Subset Of Transient Receptor Potential Vanilloid-Type Channel Subunits In Caenorhabditis Elegans Endocrine Cells Function As Mixed Heteromers To Promote Neurotransmitter Release, Antony M. Jose, Amy Bany, Daniel Chase, Michael R. Koelle
A Specific Subset Of Transient Receptor Potential Vanilloid-Type Channel Subunits In Caenorhabditis Elegans Endocrine Cells Function As Mixed Heteromers To Promote Neurotransmitter Release, Antony M. Jose, Amy Bany, Daniel Chase, Michael R. Koelle
Daniel Chase
Transient receptor potential (TRP) channel subunits form homotetramers that function in sensory transduction. Heteromeric channels also form, but their physiological subunit compositions and functions are largely unknown. We found a dominant-negative mutant of the C. elegans TRPV (vanilloid-type) subunit OCR-2 that apparently incorporates into and inactivates OCR-2 homomers as well as heteromers with the TRPV subunits OCR-1 and -4, resulting in a premature egg-laying defect. This defect is reproduced by knocking out all three OCR genes, but not by any single knockout. Thus a mixture of redundant heteromeric channels prevents premature egg laying. These channels, as well as the G-protein …
Profilin Is Essential For Tip Growth In The Moss Physcomitrella Patens, L Vidali, Rc Augustine, Kp Kleinman, M Bezanilla
Profilin Is Essential For Tip Growth In The Moss Physcomitrella Patens, L Vidali, Rc Augustine, Kp Kleinman, M Bezanilla
Magdalena Bezanilla
The actin cytoskeleton is critical for tip growth in plants. Profilin is the main monomer actin binding protein in plant cells. The moss Physcomitrella patens has three profilin genes, which are monophyletic, suggesting a single ancestor for plant profilins. Here, we used RNA interference (RNAi) to determine the loss-of-function phenotype of profilin. Reduction of profilin leads to a complete loss of tip growth and a partial inhibition of cell division, resulting in plants with small rounded cells and fewer cells. We silenced all profilins by targeting their 3′ untranslated region sequences, enabling complementation analyses by expression of profilin coding sequences. …