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Full-Text Articles in Life Sciences

River Bank Inducement Influence On A Shallow Groundwater Microbial Community And Its Effects On Aquifer Reactivity, Natalie June Gayner Dec 2018

River Bank Inducement Influence On A Shallow Groundwater Microbial Community And Its Effects On Aquifer Reactivity, Natalie June Gayner

Theses and Dissertations

Placing groundwater wells next to riverbanks to draw in surface water, known as riverbank inducement (RBI), is common and proposed as a promising and sustainable practice for municipal and public water production across the globe. However, these systems require further investigation to determine risks associated with river infiltration especially with rivers containing wastewater treatment plant (WWTP) effluent. Since microbes drive biogeochemical transformations in groundwater and largely affect water quality, it is important to understand how the microbial communities in drinking water wells are affected by river infiltration. This study investigated if, and to what extent, the microbial community in a …


Role Of The Two Adaptor Proteins In The Chp Chemosensory System Of Pseudomonas Aeruginosa, Swati Sharma May 2018

Role Of The Two Adaptor Proteins In The Chp Chemosensory System Of Pseudomonas Aeruginosa, Swati Sharma

Theses and Dissertations

The Chp chemosensory system in Pseudomonas aeruginosa controls two outputs: twitching motility (surface-mediated movement via Type IV pili) and intracellular adenosine 3’, 5’-cyclic monophosphate (cAMP) levels (by modulating the activity of major adenylate cyclase CyaB). This study was done to investigate the roles of the two adaptor proteins, PilI and ChpC in connecting one methyl-accepting protein (MCP) to one histidine kinase. We assayed -galactosidase activity as an indicator of the relative levels of intracellular cAMP and measured twitching motility. We also studied the interaction of the adaptor proteins within the Chp chemosensory system. Our bacterial adenylate cyclase two-hybrid analysis showed …