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Signature Peptide Identification For Body Fluids In Sexual Assault Cases By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Kelci Somers
Student Theses
Body fluids contain proteins that perform functions specific to different types of body fluids. Therefore, the detection of signature peptides for these proteins can potentially identify a body fluid in a forensic investigation. This project aimed to validate a method to detect signature peptides in body fluids commonly found in sexual assault cases by LC-MS/MS. Signature peptides for semen and saliva fluids were combined with two signature peptides for vaginal fluids. Samples created using two donors each for saliva, semen, and vaginal fluids were extracted using a trypsin digest, with separation of the protein and DNA fractions. The LC-MS/MS was …