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- Microbiology (2)
- 16S rRNA gene (1)
- Antibiotic Resistance (1)
- Archaea (1)
- Biofilm formation; pressure-stressed bacteria; Listeria monocytogenes; non-typhoidal Salmonella enterica serovars; Shiga toxin-producing Escherichia coli O157:H7; surface water (1)
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- Biogeochemistry (1)
- Changing Climate (1)
- Composition and distribution (1)
- Conserved signature protein (1)
- Diagnostics (1)
- Dickeya (1)
- Environmental sciences (1)
- Escherichia coli (1)
- Estuary (1)
- Freshwater (1)
- Gene expression (1)
- High-throughput sequencing (1)
- Molecular detection (1)
- Multi-function (1)
- Pectobacteriaceae (1)
- Pectobacterium (1)
- Pectobacterium versatile (1)
- Promoterless GFP (1)
- Pseudomonas coronafaciens (1)
- Soft rot Enterobacteriaceae (1)
- Soil (1)
- Staphylococcus aureus; high-pressure processing; carvacrol; nisin; caprylic acid (1)
- Tn5 transposon (1)
- Transposon mutagenesis (1)
Articles 1 - 7 of 7
Full-Text Articles in Life Sciences
Effects Of Nitrogen Fertilization And Bioenergy Crop Species On Central Tendency And Spatial Heterogeneity Of Soil Glycosidase Activities, Min Yuan, Jianjun Duan, Jianwei Li, Siyang Jian, Lahiru Gamage, Kudjo E. Dzantor, Dafeng Hui, Philip A. Fay
Effects Of Nitrogen Fertilization And Bioenergy Crop Species On Central Tendency And Spatial Heterogeneity Of Soil Glycosidase Activities, Min Yuan, Jianjun Duan, Jianwei Li, Siyang Jian, Lahiru Gamage, Kudjo E. Dzantor, Dafeng Hui, Philip A. Fay
Agricultural and Environmental Sciences Faculty Research
Extracellular glycosidases in soil, produced by microorganisms, act as major agents for decomposing labile soil organic carbon (e.g., cellulose). Soil extracellular glycosidases are significantly affected by nitrogen (N) fertilization but fertilization effects on spatial distributions of soil glycosidases have not been well addressed. Whether the effects of N fertilization vary with bioenergy crop species also remains unclear. Based on a 3-year fertilization experiment in Middle Tennessee, USA, a total of 288 soil samples in topsoil (0–15 cm) were collected from two 15 m2 plots under three fertilization treatments in switchgrass (SG: Panicum virgatum L.) and gamagrass (GG: Tripsacum dactyloides L.) …
A Mini-Tn5-Derived Transposon With Reportable And Selectable Markers Enables Rapid Generation And Screening Of Insertional Mutants In Gram-Negative Bacteria, Eric S. Nazareno, Bimala Acharya, C. Korsi Dumenyo
A Mini-Tn5-Derived Transposon With Reportable And Selectable Markers Enables Rapid Generation And Screening Of Insertional Mutants In Gram-Negative Bacteria, Eric S. Nazareno, Bimala Acharya, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
We re-engineered a classic tool for mutagenesis and gene expression studies in Gram-negative bacteria. Our modified Tn5-based transposon contains multiple features that allow rapid selection for mutants, direct quantification of gene expression and straightforward cloning of the inactivated gene. The promoter-less gfp-km cassette provides selection and reporter assay depending on the activity of the promoter upstream of the transposon insertion site. The cat gene facilitates positive antibiotic selection for mutants, while the narrow R6Kγ replication origin forces transposition in recipient strains lacking the pir gene and enables cloning of the transposon flanked with the disrupted gene from the chromosome. The …
Distinct Distribution Of Archaea From Soil To Freshwater To Estuary: Implications Of Archaeal Composition And Function In Different Environments, Hualong Wang, Raven Bier, Laura Zgleszewski, Marc Peipoch, Emmanuel Omondi, Atanu Mukherjee, Feng Chen, Chuanlun Zhang, Jinjun Kan
Distinct Distribution Of Archaea From Soil To Freshwater To Estuary: Implications Of Archaeal Composition And Function In Different Environments, Hualong Wang, Raven Bier, Laura Zgleszewski, Marc Peipoch, Emmanuel Omondi, Atanu Mukherjee, Feng Chen, Chuanlun Zhang, Jinjun Kan
Agricultural and Environmental Sciences Faculty Research
In addition to inhabiting extreme territories, Archaea are widely distributed in common environments spanning from terrestrial to aquatic environments. This study investigated and compared archaeal community structures from three different habitats (representing distinct environments): agriculture soils (from farming system trials FST, PA, United States), freshwater biofilms (from White Clay Creek, PA, United States), and estuary water (Chesapeake Bay, United States). High-throughput sequencing of 16S rRNA genes indicated that Thaumarchaeota, Euryarchaeota, Nanoarchaeota, Crenarchaeota, and Diapherotrites were the commonly found dominant phyla across these three environments. Similar to Bacteria, distinct community structure and distribution patterns for Archaea were observed in soils vs. …
Sensitivity Of Planktonic Cells Of Staphylococcus Aureus To Elevated Hydrostatic Pressure As Affected By Mild Heat, Carvacrol, Nisin, And Caprylic Acid, Jyothi George, Sadiye Aras, Md Niamul Kabir, Sabrina Wadood, Shahid Chowdhury, Aliyar Cyrus Fouladkhah
Sensitivity Of Planktonic Cells Of Staphylococcus Aureus To Elevated Hydrostatic Pressure As Affected By Mild Heat, Carvacrol, Nisin, And Caprylic Acid, Jyothi George, Sadiye Aras, Md Niamul Kabir, Sabrina Wadood, Shahid Chowdhury, Aliyar Cyrus Fouladkhah
Agricultural and Environmental Sciences Faculty Research
Current study investigated effects of elevated hydrostatic pressure exposure in the presence of mild heat and natural antimicrobials against Staphylococcus aureus. Hydrostatic pressure of 350 to 550 MPa with nisin (5000 IU/mL), carvacrol, or caprylic acid (0.5% v/v) were applied for the reduction in four-strain mixture of S. aureus in HEPES buffer at 4 and 40 °C for up to 7 min. Results were statistically analyzed by ANOVA and D-values were additionally calculated using best-fitted linear model. Prior to exposure to treatments at 4 °C, counts of the pathogen were 7.95 ± 0.4 log CFU/mL and were reduced (p < 0.05) to 6.44 ± 0.3 log CFU/mL after 7 min of treatment at 450 MPa. D-value associated with this treatment was 5.34 min (R2 …
The Threat Of Antibiotic Resistance In Changing Climate, Aliyar Cyrus Fouladkhah, Brian Thompson, Janey Smith Camp
The Threat Of Antibiotic Resistance In Changing Climate, Aliyar Cyrus Fouladkhah, Brian Thompson, Janey Smith Camp
Agricultural and Environmental Sciences Faculty Research
As the earliest form of life, microorganisms have elaborate mechanisms for adapting to changes in environmental conditions.
Fate And Biofilm Formation Of Wild-Type And Pressure-Stressed Pathogens Of Public Health Concern In Surface Water And On Abiotic Surfaces, Md Niamul Kabir, Sadiye Aras, Sabrina Wadood, Shahid Chowdhury, Aliyar Cyrus Fouladkhah
Fate And Biofilm Formation Of Wild-Type And Pressure-Stressed Pathogens Of Public Health Concern In Surface Water And On Abiotic Surfaces, Md Niamul Kabir, Sadiye Aras, Sabrina Wadood, Shahid Chowdhury, Aliyar Cyrus Fouladkhah
Agricultural and Environmental Sciences Faculty Research
Since the historic outbreak near Broad Street in London, which serves as cornerstone of modern epidemiology, infectious diseases spread in surface and sub-surface water has been a persisting public health challenge. The current study investigated persistence of wild-type and pressure-stressed Listeria monocytogenes, Escherichia coli O157:H7, and non-typhoidal Salmonella enterica serovars in surface water stored aerobically for up to 28 days at 5, 25, and 37 °C. Additionally, biofilm formation of wild-type and pressure-stressed non-typhoidal Salmonella serovars were monitored on surface of stainless steel and rubber coupons for 28 days at 25 and 37 °C. While L. monocytogenes exhibited a lower …
Development Of Pcr-Based Detection System For Soft Rot Pectobacteriaceae Pathogens Using Molecular Signatures, Md Niamul Kabir, Ali Taheri, C. Korsi Dumenyo
Development Of Pcr-Based Detection System For Soft Rot Pectobacteriaceae Pathogens Using Molecular Signatures, Md Niamul Kabir, Ali Taheri, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
Pectobacterium and Dickeya species, usually referred to as soft rot Enterobacteriaceae, are phytopathogenic genera of bacteria that cause soft rot and blackleg diseases and are responsible for significant yield losses in many crops across the globe. Diagnosis of soft rot disease is difficult through visual disease symptoms. Pathogen detection and identification methods based on cultural and morphological identification are time-consuming and not always reliable. A polymerase chain reaction (PCR)-based detection method with the species-specific primers is fast and reliable for detecting soft rot pathogens. We have developed a specific and sensitive detection system for some species of soft rot Pectobacteriaceae …