Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- Bacteriology (2)
- Blue-green bacteria (2)
- Cyanobacteria (2)
- Microbiology (2)
- Molecular interaction (2)
-
- Papovaviridae (2)
- Polyomavirus (2)
- Sv40 virus (2)
- T antigen (2)
- Transcription (2)
- Virus (2)
- Activation (1)
- Antigène t (1)
- Arthropoda (1)
- Cell transformation (1)
- Diptera (1)
- Dna (1)
- Drosophila melanogaster (1)
- Drosophilidae (1)
- Ecdysteroide (1)
- Embryo (1)
- Expression génique (1)
- Falciparum malaria (1)
- Fibroblast (1)
- Gene expression (1)
- Genetic engineering (1)
- Hepatic dysfunction (1)
- Insecta (1)
- Interaction moléculaire (1)
- Invertebrata (1)
Articles 1 - 14 of 14
Full-Text Articles in Life Sciences
The Ability Of Simian Virus 40 Large T Antigen To Immortalize Primary Mouse Embryo Fibroblasts Cosegregates With Its Ability To Bind To P53., Jiyue Y. Zhu, Marina Abate, Philip W. Rice, Charles N. Cole
The Ability Of Simian Virus 40 Large T Antigen To Immortalize Primary Mouse Embryo Fibroblasts Cosegregates With Its Ability To Bind To P53., Jiyue Y. Zhu, Marina Abate, Philip W. Rice, Charles N. Cole
Dartmouth Scholarship
The large T antigen encoded by simian virus 40 (SV40) plays essential roles in the infection of permissive cells, leading to production of progeny virions, and in the infection of nonpermissive cells, leading to malignant transformation. Primary mouse embryo fibroblasts (MEFs) are nonpermissive for SV40, and infection by wild-type SV40 leads to immortalization and transformation of a small percentage of infected cells. We examined the ability of an extensive set of mutants whose lesions affect SV40 large T antigen to immortalize MEFs. We found that immortalization activity was retained by all mutants whose lesions are located upstream of codon 346. …
Hepatic Dysfunction In Falciparum Malaria, J Khan, J Akhter, H Sheikh, W Jafri
Hepatic Dysfunction In Falciparum Malaria, J Khan, J Akhter, H Sheikh, W Jafri
Department of Pathology and Laboratory Medicine
No abstract provided.
Nutritional Complementation Of Oxidative Glucose Metabolism In Escherichia Coli Via Pyrroloquinoline Quinone-Dependent Glucose Dehydrogenase And The Entner-Doudoroff Pathway, Michael Adamowicz, Tyrrell Conway, Kenneth W. Nickerson
Nutritional Complementation Of Oxidative Glucose Metabolism In Escherichia Coli Via Pyrroloquinoline Quinone-Dependent Glucose Dehydrogenase And The Entner-Doudoroff Pathway, Michael Adamowicz, Tyrrell Conway, Kenneth W. Nickerson
Papers in Microbiology
Two glucose-negative Escherichia coli mutants (ZSC113 and DF214) were unable to grow on glucose as the sole carbon source unless supplemented with pyrroloquinoline quinone (PQQ). PQQ is the cofactor for the periplasmic enzyme glucose dehydrogenase, which converts glucose to gluconate. Aerobically, E. coli ZSC113 grew on glucose plus PQQ with a generation time of 65 min, a generation time about the same as that for wildtype E. coli in a defined glucose-salts medium. Thus, for E. coli ZSC113 the Entner-Doudoroff pathway was fully able to replace the Embden-Meyerhof-Parnas pathway. In the presence of 5% sodium dodecyl sulfate, PQQ no longer …
The Inability Of Human Immunodeficiency Virus To Infect Chimpanzee Monocytes Can Be Overcome By Serial Viral Passage In Vivo, Howard Gendelman, Garth D. Ehrlich, Lisa M. Baca, Shawn Conley, Jorge Ribas, D. Chester Kalter, Monte S. Meltzer, Bernard J. Poiesz, Peter Nara
The Inability Of Human Immunodeficiency Virus To Infect Chimpanzee Monocytes Can Be Overcome By Serial Viral Passage In Vivo, Howard Gendelman, Garth D. Ehrlich, Lisa M. Baca, Shawn Conley, Jorge Ribas, D. Chester Kalter, Monte S. Meltzer, Bernard J. Poiesz, Peter Nara
Nebraska Center for Virology: Faculty Publications
Studies of lentivirus infection in ruminants, nonhuman primates, and humans suggest that virus infection of macrophages plays a central role in the disease process. To investigate whether human immunodeficiency virus type 1 (HIV-1) can infect chimpanzee macrophages, we recovered monocytes from peripheral blood mononuclear cells of HIV-1-negative animals and inoculated these and control human monocytes with a panel of four human-passaged monocytotropic virus strains and one chimpanzee-passaged isolate. HIV-1-infected human monocytes synthesized proviral DNA, viral mRNA, p24 antigen, and progeny virions. In contrast, except for the chimpanzee-passaged HIV-1 isolate, chimpanzee monocytes failed to support HIV-1 replication when cultured under both …
Mapping The Transcriptional Transactivation Function Of Simian Virus 40 Large T Antigen., Jiyue Y. Zhu, Philip W. Rice, Michele Chamberlain, Charles N. Cole
Mapping The Transcriptional Transactivation Function Of Simian Virus 40 Large T Antigen., Jiyue Y. Zhu, Philip W. Rice, Michele Chamberlain, Charles N. Cole
Dartmouth Scholarship
T antigen is able to transactivate gene expression from the simian virus 40 (SV40) late promoter and from several other viral and cellular promoters. Neither the mechanisms of transactivation by T antigen nor the regions of T antigen required for this activity have been determined. To address the latter point, we have measured the ability of a set of SV40 large T antigen mutants to stimulate gene expression in CV-1 monkey kidney cells from the SV40 late promoter and Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter. Transactivation, although reduced, was retained by an N-terminal 138-amino-acid fragment of T …
Transactivation Of The Human Immunodeficiency Virus Promoter By Human Herpesvirus 6 (Hhv-6) Strains Gs And Z-29 In Primary Human T Lymphocytes And Identification Of Transactivating Hhv-6(Gs) Gene Fragments, Rebecca Horvat, Charles Wood, Steven Josephs, N. Balanchandran
Transactivation Of The Human Immunodeficiency Virus Promoter By Human Herpesvirus 6 (Hhv-6) Strains Gs And Z-29 In Primary Human T Lymphocytes And Identification Of Transactivating Hhv-6(Gs) Gene Fragments, Rebecca Horvat, Charles Wood, Steven Josephs, N. Balanchandran
Nebraska Center for Virology: Faculty Publications
Human herpesvirus 6 (HHV-6) can activate the human immunodeficiency virus (HIV) promoter and accelerate cytopathic effects in HIV-infected human T cells. This study examines the regions of the HIV promoter required for HHV-6 transactivation in a heterogeneous population of primary human T lymphocytes with or without antigenic stimulation. Two different strains of HHV-6, GS and Z29, transactivated the HIV promoter. The GS strain transactivated the promoter in both stimulated and resting T cells, while the Z29 strain increased HIV promoter activity only in stimulated T cells. Three DNA clones containing HHV-6(GS) genomic fragments transactivated the HIV promoter in cotransfected T …
Transactivation Of The Human Immunodeficiency Virus Promoter By Human Herpesvirus 6 (Hhv-6) Strains Gs And 2-29 In Primary Human T Lymphocytes And Identification Of Transactivating Hhv-6(Gs) Gene Fragments, Rebecca Horvat, Charles Wood, Steven Josephs, N. Balachandran
Transactivation Of The Human Immunodeficiency Virus Promoter By Human Herpesvirus 6 (Hhv-6) Strains Gs And 2-29 In Primary Human T Lymphocytes And Identification Of Transactivating Hhv-6(Gs) Gene Fragments, Rebecca Horvat, Charles Wood, Steven Josephs, N. Balachandran
Nebraska Center for Virology: Faculty Publications
Human herpesvirus 6 (HHV-6) can activate the human immunodeficiency virus (HIV) promoter and accelerate cytopathic effects in HIV-infected human T cells. This study examines the regions of the HIV promoter required for HHVd transactivation in a heterogeneous population of primary human T lymphocytes with or without antigenic stimulation. Two different strains of HHV-6, GS and 229, transactivated the HIV promoter. The GS strain transactivated the promoter in both stimulated and resting T cells, while the 229 strain increased HIV promoter activity only in stimulated T cells. Three DNA clones containing HHV-6(GS) genomic fragments transactivated the HIV promoter in cotransfected T …
Ecdysterone Regulatory Elements Function As Both Transcriptional Activators And Repressors., Leonard Dobens, Karen Rudolph, Edward M. Berger
Ecdysterone Regulatory Elements Function As Both Transcriptional Activators And Repressors., Leonard Dobens, Karen Rudolph, Edward M. Berger
Dartmouth Scholarship
A synthetic, 23-bp ecdysterone regulatory element (EcRE), derived from the upstream region of the Drosophila melanogaster hsp27 gene, was inserted adjacent to the herpes simplex virus thymidine kinase promoter fused to a bacterial gene for chloramphenicol acetyltransferase (CAT). Hybrid constructs were transfected into Drosophila S3 cells and assayed for ecdysterone-inducible CAT expression. In the absence of ecdysterone a tandem pair of EcREs repressed the high constitutive level of CAT activity found after transfection with the parent reporter plasmid alone. After hormone addition very high levels of CAT activity were observed. Insertion of the EcRE pair 3' of the CAT gene …
Strain-Specific Neutralizing Determinant In The Transmembrane Protein Of Simian Immunodeficiency Virus, Toshiaki Kodama, Dawn P. Wooley, Daniel P. Silva, Fulvia Dimarzo Veronese, Ronald C. Desrosiers
Strain-Specific Neutralizing Determinant In The Transmembrane Protein Of Simian Immunodeficiency Virus, Toshiaki Kodama, Dawn P. Wooley, Daniel P. Silva, Fulvia Dimarzo Veronese, Ronald C. Desrosiers
Neuroscience, Cell Biology & Physiology Faculty Publications
Monoclonal antibody SF8/5E11, which recognizes the transmembrane protein (TMP) of simian immunodeficiency virus of macaque monkeys (SIVmac), displayed strict strain specificity. It reacted with cloned and uncloned SIVmac251 but not with cloned SIVmac142 and SIVmac239 on immunoblots. This monoclonal antibody neutralized infection by cloned, cell-free SIVmac251 and inhibited formation of syncytia by cloned SIVmac251-infected cells; these activities were specific to cloned SIVmac251 and did not occur with the other viruses. Site-specific mutagenesis was used to show that TMP amino acids 106 to 110 (Asp-Trp-Asn-Asn-Asp) determined the strain specificity of the monoclonal antibody. This strain-specific neutralizing determinant is located within a …
Selection Of Genetic Variants Of Simian Immunodeficiency Virus In Persistently Infected Rhesus Monkeys, Dawn P. Wooley, Ronald C. Desrosiers
Selection Of Genetic Variants Of Simian Immunodeficiency Virus In Persistently Infected Rhesus Monkeys, Dawn P. Wooley, Ronald C. Desrosiers
Neuroscience, Cell Biology & Physiology Faculty Publications
Genetic and antigenic variation may be one means by which lentiviruses that cause AIDS avoid elimination by host immune responses. Genetic variation in the envelope gene (env) was studied by comparing the nucleotide sequences of 27 clones obtained from two rhesus monkeys infected with molecularly cloned simian immunodeficiency virus. All 27 clones differed from each other and differed from the input clone in the gp120 (SU) portion of the envelope gene. Nucleotide substitutions were shown to accumulate with time at an average rate of 8.5 per 1,000 per year in SU. Surprisingly, the majority of nucleotide substitutions (81%) resulted in …
Rapid Detection Of Bovine Viral Diarrhea Virus By Polymerase Chain Reaction, O. J. Lopez, Fernando A. Osorio, Ruben O. Donis
Rapid Detection Of Bovine Viral Diarrhea Virus By Polymerase Chain Reaction, O. J. Lopez, Fernando A. Osorio, Ruben O. Donis
Nebraska Center for Virology: Faculty Publications
The polymerase chain reaction was used to detect genomic sequences of the positive-stranded RNA of bovine viral diarrhea virus (BVDV), a member of the family Togaviridae. Using a set of 20-bp primers located within the conserved 3' region of the BVDV genome, we were able to consistently amplify a 205-bp target sequence from BVDV cDNA. BVDV RNAs from cell culture-propagated BVDV reference strains, diverse unrelated cytopathic and noncytopathic field isolates, and clinical serum samples were transcribed to cDNA by using avian myeloblastosis virus reverse transcriptase and further specifically amplified by using the polymerase chain reaction assay. The amplification assay …
Detergent (Sodium Dodecyl Sulfate) Shock Proteins In Escherichia Coli, Michael Adamowicz, Philip M. Kelley, Kenneth W. Nickerson
Detergent (Sodium Dodecyl Sulfate) Shock Proteins In Escherichia Coli, Michael Adamowicz, Philip M. Kelley, Kenneth W. Nickerson
Papers in Microbiology
The protein composition of Escherichia coli W3110 grown in the presence and absence of 5% sodium dodecyl sulfate (SDS) was examined by two-dimensional gel electrophoresis. In SDS-grown cells, at least 4 proteins were turned on, 13 were turned off, 15 were elevated, and 15 were depressed. The 19 unique and elevated SDS-induced spots constituted 7.91% of the total 35S-labeled protein. There was no apparent overlap between these 19 detergent (SDS) stress proteins and those of other known bacterial stress responses. The detergent stress stimulon is a distinct and independent stimulon. Its physiological relevance probably derives from the presence of …
Cyanonews (Vol. 7, No. 1, April 1991), Jeff Elhai
Cyanonews (Vol. 7, No. 1, April 1991), Jeff Elhai
CyanoNews
CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.
Cyanonews (Vol. 7, No. 2, September 1991), Jeff Elhai
Cyanonews (Vol. 7, No. 2, September 1991), Jeff Elhai
CyanoNews
CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.