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Whole Genome Sequence Analysis Of A Transmissible Multidrug-Resistance Plasmid Captured Without Cultivation From Poultry Litter, Emma C. Eisemann
Whole Genome Sequence Analysis Of A Transmissible Multidrug-Resistance Plasmid Captured Without Cultivation From Poultry Litter, Emma C. Eisemann
Senior Honors Projects, 2020-current
Use of antibiotics in the agricultural industry introduces selective pressure and, consequently, could increase the presence of antibiotic resistant organisms in surrounding environments. One such environment is litter (manure and bedding) produced during large-scale poultry production in the Shenandoah Valley. Litter, with its microorganisms, is commonly applied to fields within the Shenandoah River watershed. Antibiotic resistance (AR) and virulence genes are potentially transmissible between organisms through horizontal gene transfer of genetic mobile elements, for which poultry litter could be a reservoir. The typical, culture-based approach to detecting and analyzing AR plasmids and other mobile genetic elements is limited due to …