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- Aegle marmelos (4)
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Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Differential Expression Of Gastric Muc5ac In Colonic Epithelial Cells: Tff-3 Wired Il1Β/Akt Crosstalk Induced Mucosal Immune Response Against Shigella Dysenteriae Infection, Raja Sb, Murali Mr, Devaraj H, Devaraj Sn
Differential Expression Of Gastric Muc5ac In Colonic Epithelial Cells: Tff-3 Wired Il1Β/Akt Crosstalk Induced Mucosal Immune Response Against Shigella Dysenteriae Infection, Raja Sb, Murali Mr, Devaraj H, Devaraj Sn
Murali Malliga Raman
An understanding of the signaling mechanism(s) that regulate the differential expression of gastric mucin MUC5AC in colonic epithelial cells would contribute significantly to investigations of its role in colonic mucosa infected with the bacterial pathogen Shigella dysenteriae. Here we show that S. dysenteriae-Sinduced expression of interleukin-1β upregulates MUC2 expression and the differential expression of MUC5AC. Differential expression of MUC5AC involves crosstalk between interleukin-1β and Akt, whereby the trefoil factor family peptide TFF3 activates Akt by phosphorylation of EGFR. TFF3 also downregulates E-cadherin expression, causing accumulation of β-catenin in the cytosol. Phosphorylation of GSK-3β (inactivated) by activated Akt inhibits ubiquitylation of …
Isolation And Partial Characterization Of A Novel Lectin From Aegle Marmelos Fruit And Its Effect On Adherence And Invasion Of Shigella To Ht29 Cells, Raja Sb, Murali Mr, Kumar Nk, Devaraj Sn
Isolation And Partial Characterization Of A Novel Lectin From Aegle Marmelos Fruit And Its Effect On Adherence And Invasion Of Shigella To Ht29 Cells, Raja Sb, Murali Mr, Kumar Nk, Devaraj Sn
Murali Malliga Raman
Lectins are a class of ubiquitous proteins/glycoproteins that are abundantly found in nature. Lectins have unique carbohydrate binding property and hence have been exploited as drugs against various infectious diseases. We have isolated one such novel lectin from the fruit pulp of Aegle marmelos. The isolated lectin was partially characterised and its effect against Shigella dysenteriae infection was evaluated. The isolated lectin was found to be a dimeric protein with N-acetylgalactosamine, mannose and sialic acid binding specificity. The effect of Aegle marmelos fruit lectin on the adherence of Shigella dysenteriae to human colonic epithelial cells (HT29 cells) was evaluated by …
Imperatorin A Furocoumarin Inhibits Periplasmic Cu-Zn Sod Of Shigella Dysenteriae Their By Modulates Its Resistance Towards Phagocytosis During Host Pathogen Interaction, Raja Sb, Murali Mr, Roopa K, Devaraj Sn
Imperatorin A Furocoumarin Inhibits Periplasmic Cu-Zn Sod Of Shigella Dysenteriae Their By Modulates Its Resistance Towards Phagocytosis During Host Pathogen Interaction, Raja Sb, Murali Mr, Roopa K, Devaraj Sn
Murali Malliga Raman
Shigella dysenteriae continues to be a major health problem, which leads to death, due to diarrhoea and dysentery, predominantly in children below the age of 5. Bacterial invasion of the colonic epithelium leads to severe inflammation together with bacterial dissemination generates abscesses and ulcerations. Periplasmic copper, zinc super oxide dismutase of Shigella protects it from exogenous superoxide produced by host, during its invasion. Hence, in present study an attempt was made to study the effect of aqueous extract of Aegle marmelos on host and pathogen defence. Histology analysis of rat ileal loop showed the loss of virulence in aqueous extract …
Neutralization Of Radical Toxicity By Temperature-Dependent Modulation Of Extracellular Sod Activity In Coral Bleaching Pathogen Vibrio Shiloi And Its Role As A Virulence Factor, Murali Mr, Raja Sb, Devaraj Sn
Neutralization Of Radical Toxicity By Temperature-Dependent Modulation Of Extracellular Sod Activity In Coral Bleaching Pathogen Vibrio Shiloi And Its Role As A Virulence Factor, Murali Mr, Raja Sb, Devaraj Sn
Murali Malliga Raman
Vibrio shiloi is the first and well-documented bacterium which causes coral bleaching, particularly, during summer, when seawater temperature is between 26 and 31 degrees C. Coral bleaching is the disruption of the symbiotic association between coral hosts and their photosynthetic microalgae zooxanthellae. This is either due to lowered resistance in corals to infection or increased virulence of the bacterium at the higher sea surface temperature. The concentration of the oxygen and resulting oxygen radicals produced by the zooxanthellae during photosynthesis are highly toxic to bacteria, which also assist corals in resisting the infection. Hence, in this study we examined the …
Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S
Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S
Murali Malliga Raman
OBJECTIVE: Shigella dysenteriae Type 1 dysentery is a major cause of morbidity and mortality in children from less developed and developing countries. The present study explores the hypothesis that lactobacilli protect the host cell during S. dysenteriae Type 1 infection and its mechanism of action. METHODS: Caco-2 cells incubated for 1h with Lactobacillus rhamnosus or Lactobacillus acidophilus at the multiplicity of infection of 100, either alone or in combination followed by addition of Shigella at the same multiplicity of infection for 5h served as treatment groups. Cells incubated with Shigella without lactobacilli addition served as infected cells. At the end …
Effect Of Aqueous Extract Of Aegle Marmelos Fruit On Adherence And Β-Lactam Resistance Of Enteropathogenic Escherichia Coli By Down Regulating Outer Membrane Protein C, Murali Malliga Raman
Effect Of Aqueous Extract Of Aegle Marmelos Fruit On Adherence And Β-Lactam Resistance Of Enteropathogenic Escherichia Coli By Down Regulating Outer Membrane Protein C, Murali Malliga Raman
Murali Malliga Raman
Problem statement: Enteropathogenic Escherichia Coli (EPEC) continue to be a major health problem, leading to death due to diarrhea, predominantly in children below the age of five. Due to evolution of multi drug resistance in EPEC and side effects caused to host by antibiotics necessitated a search for alternative medicines from medicinal plants. One such medicinal plant used since ancient times to cure diarrhea is Aegle marmelos. This study was done to investigate the effect of aqueous extract of Aegle marmelos fruit (AEAM) on outer membrane protein C (OmpC) of EPEC, which plays a key role in adherence and antibiotic …
Differential Expression Of Ompc And Ompf In Multidrug-Resistant Shigella Dysenteriae And Shigella Flexneri By Aqueous Extract Of Aegle Marmelos, Altering Its Susceptibility Toward Beta-Lactam Antibiotics, Raja Sb, Murali Mr, Devaraj Sn
Differential Expression Of Ompc And Ompf In Multidrug-Resistant Shigella Dysenteriae And Shigella Flexneri By Aqueous Extract Of Aegle Marmelos, Altering Its Susceptibility Toward Beta-Lactam Antibiotics, Raja Sb, Murali Mr, Devaraj Sn
Murali Malliga Raman
Steadily increasing resistance among Shigella to beta-lactams, aminoglycosides, and tetracycline has compromised the utility of these commonly used antimicrobial agents. Also, undesirable side effects of certain antibiotics have triggered immense interest in search of alternative therapies using medicinal plants. One such medicinal plant used since ancient times to cure diarrhea is Aegle marmelos. The present study exemplifies the susceptibility of beta-lactam-resistant Shigella dysenteriae and Shigella flexneri toward beta-lactam antibiotics, when grown in the presence of aqueous extract of A. marmelos (AEAM), by altering porin channels. This was demonstrated by antibiotic sensitivity test using disc diffusion method and MIC test. Susceptibility …
Protective Role Of Lactobacilli In Shigella Dysenteriae 1-Induced Diarrhea In Rats., Moorthy G, Murali Malliga Raman, Devaraj Sn
Protective Role Of Lactobacilli In Shigella Dysenteriae 1-Induced Diarrhea In Rats., Moorthy G, Murali Malliga Raman, Devaraj Sn
Murali Malliga Raman
OBJECTIVE: Studies on lactic acid bacteria exemplify their use against various enteropathogens in vitro. Nevertheless, in vivo effects of Lactobacillus during Shigella infection have not been evaluated. The present study evaluated the effect of Lactobacillus rhamnosus and Lactobacillus acidophilus on neutrophil infiltration and lipid peroxidation during Shigella dysenteriae 1-induced diarrhea in rats. METHODS: The rats were divided into eight groups (n = 6 in each group). Induced rats received single oral dose of S. dysenteriae (12 x 10(8) colony-forming units [cfu]/mL). Treated rats received L. rhamnosus (1 x 10(7) cfu/mL) or L. acidophilus (1 x 10(7) cfu/mL) orally for 4 …